Method for improving flavonoid phenylpropanoid compounds of saussurea involucrate cell culture

Abstract

The invention belongs to the field of plant biotechnology engineering, and particularly relates to a method for improving flavonoid phenylpropanoid compounds of a saussurea involucrate cell culture. In a culture medium used in the method, the phosphorus element concentration is 0.5-3 mmol / L, the NO3-ion concentration is 20-35 mmol / L, the NH4+ ion concentration is 0-30 mmol / L, and the Ca 2+ ion concentration is 0.5-3 mmol / L; the Mg 2+ ion concentration is 0.2-1.5 mmol / L, and the concentration of boron ions is 0.02-0.1 mmol / L; inducers or precursors or inhibitors of bypass metabolism may be added to the culture medium. Finally, the total flavone content of the saussurea involucrate cell culture and the content of rutin, chlorogenic acid, Syringin and 1, 5-dicaffeoylquinic acid are obviouslyimproved.

Description

technical field [0001] The invention belongs to the field of plant biotechnology engineering, relates to a tissue culture of a medicinal plant and a culture method thereof, in particular to a method for increasing flavonoid phenylpropanoids in snow lotus cell culture. Background technique [0002] Snow lotus is a folk medicinal plant in high mountain areas. It is used for dispelling cold and dehumidification, promoting blood circulation and stimulating menstruation, anti-inflammatory and analgesic, etc. It is mostly used in folk for rheumatoid arthritis, cold pain in women's lower abdomen, amenorrhea, retained placenta, impermeable numbness, and cold lung Treatment of cough, impotence, and altitude sickness. In recent years, Snow Lotus, as a national medicine, has attracted much attention for its anti-inflammatory and analgesic effects, anti-early pregnancy, anti-aging and inhibition of cancer cell proliferation. But my country's snow lotus is mainly distributed in the plat...

AI Technical Summary

Problems solved by technology

[0005] 1. The obtained culture has a single component, mostly with a single total flavonoid as the index, and the content is low, which makes it difficult to realize the true value of Saussurea sauraceae; 2. For the problem of low content of certain components, no effective means of obtaining high-yield cell lines has been found etc.; 3. The inducer of the culture is only to obtain higher total flavonoids, while ignoring other components of Saussurea sauraceae itself; 4. In the metabolic regulation, only the addition of the inducer is paid attention to, without changing the flow direction of the branch metabolic pathway, blocking The synthesis of metabolites is not required to achieve a method for increasing product yield; 5. The culture process is mostly a common basic medium, but there is no special basic medium for Saussurea edulis according to the characteristics of Saussurea chinensis and the quality of the culture; 6. Most of the cultures Staying in the laboratory, it is difficult to cultivate on a large scale, and it is unstable during the enlargement process. When the enlargement is cultivated to a certain scale, the content of active ingredients will decrease; A lot of work has been done on cultivation, fermentation and cultivation, etc. However, the cultivation effect of Saussurea sauraceae cells and the medicinal ingredients of Saussurea sauraceae culture are single, and the improvement of the content of main medicinal ingredients needs to be explored

The lack of optimization of the basic culture environment and the lack of regulation of metabolic pathways lead to the lack of a stable, high-yield and rapid large-scale cultivation of Snow Lotus cells.

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