The invention relates to a preparation method of a eurotium cristatum 
mycelium extract, and mainly relates to a process for liquid 
fermentation of eurotium cristatum, collection of 
mycelium and extraction of active ingredients. A 
fermentation culture medium of the eurotium cristatum uses tea 
powder as a nutritional ingredient and a dispersion medium to promote the 
pelletizing property of the eurotium cristatum 
mycelium, so that the nutritional ingredients in the culture medium are used and converted to the maximum. Through 
fermentation culture in a 1L fermentation tank, the 
dry weight of the mycelium can be up to 15g / L. The fermentation liquor is centrifuged to collect the mycelium, then the mycelium is subjected to weight suspending by using water and then is twisted into fragments, a wall-breaking 
enzyme is added and uniformly mixed with the mycelium, and then the mixture is subjected to high-pressure homogenization and 
alcohol precipitation to extract the active ingredients in the mycelium. The preparation method improves the existing relative processes, so that the extraction rate of the active ingredients in the mycelium is improved, and large-scale production can be realized. The active ingredients in the mycelium mainly comprise 
polysaccharide, and have better effects on reducing fat and improving the 
stomach and intestines.