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Preparation method of eurotium cristatum mycelium extract

A technology of coronoid saccharomyces and mycelium, applied in the biological field, can solve the problems of high cost, unsuitable for the development of food-grade health products, unsuitable for large-scale production, etc., and achieve the effect of increasing the biomass of bacteria

Inactive Publication Date: 2013-09-18
湖南惟楚福瑞达生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the ultrasonic method has a high extraction rate, it is only suitable for small batch experiments and is not suitable for large-scale production
Chloroform has the advantage of high protein removal efficiency, but it is toxic and costly, and the products produced are not suitable for the development of food-grade health products

Method used

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  • Preparation method of eurotium cristatum mycelium extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of Mycelium of Mycocystis coronarii

[0029] Coronoidis spread on PDA agar medium plate, cultured at 30°C for 6 days, add an appropriate amount of sterile water to the plate, scrape off the spores with a sterile scraper, suspend in sterile water, and then dissolve the suspension Pour it into a conical flask containing glass beads, oscillate on a shaker at 200r / min for 1h to make a spore suspension, count on a cell counting board, and control the spore concentration to 1×10 8 cfu / mL.

[0030] The formula of the fermentation medium is: sucrose 20g / L, tea powder 8g / L, pH 5.5, sterilized at 115°C for 30min.

[0031] The tea powder of the present invention is high-quality black hair tea which is pulverized by a pulverizer and passed through a 60-mesh sieve.

[0032] The fermentation process is: the concentration is 1×10 8 The cfu / mL coronoid spore liquid is inserted into a 1L fermenter containing 800mL fermentation broth at an inoculum of 2%, and the pH is adjus...

Embodiment 2

[0035] Preparation of Mycelium of Mycocystis coronarii

[0036] Coronoidis spread on PDA agar medium plate, cultured at 30°C for 8 days, add an appropriate amount of sterile water to the plate, scrape off the spores with a sterile scraper, suspend in sterile water, and then dissolve the suspension Pour into a conical flask containing glass beads, shake on a shaker at 200r / min for 1h to make a spore suspension, count on a cell counting board, and control the spore concentration to 2×10 8 cfu / mL.

[0037] The fermentation medium formula is: glucose 60g / L, tea powder 8g / L, yeast extract 5g / L, NaH 2 PO 4 1.2g / L, K 2 HPO 4 3g / L, MgSO 4 ·7H 2 O0.1g / L, ZnSO 4 ·7H 2 O0.01g / L, pH5.5, sterilized at 115°C for 30min.

[0038] The tea powder of the present invention is high-quality black hair tea which is pulverized by a pulverizer and passed through a 60-mesh sieve.

[0039] The fermentation process is as follows: insert the spore liquid of S. coronoidis into a 1L fermenter cont...

Embodiment 3

[0042] Preparation of Mycelium of Mycocystis coronarii

[0043] Coronoidis spread on PDA agar medium plate, cultured at 30°C for 8 days, add an appropriate amount of sterile water to the plate, scrape off the spores with a sterile scraper, suspend in sterile water, and then dissolve the suspension Pour it into a conical flask containing glass beads, oscillate on a shaker at 200r / min for 1h to make a spore suspension, count on a cell counting board, and control the spore concentration to 5×10 8 cfu / mL.

[0044] The formula of the fermentation medium is: sucrose 40g / L, tea powder 8g / L, yeast extract 5g / L, NaH 2 PO 4 1.2g / L, K 2 HPO 4 3g / L, MgSO 4 ·7H 2 O0.1g / L, ZnSO 4 ·7H 2 O0.01g / L, pH5.0, sterilized at 115°C for 30min.

[0045] The tea powder of the present invention is high-quality black hair tea which is pulverized by a pulverizer and passed through a 120-mesh sieve.

[0046] The fermentation process is as follows: put the spore liquid of S. coronis into a 1L ferme...

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Abstract

The invention relates to a preparation method of a eurotium cristatum mycelium extract, and mainly relates to a process for liquid fermentation of eurotium cristatum, collection of mycelium and extraction of active ingredients. A fermentation culture medium of the eurotium cristatum uses tea powder as a nutritional ingredient and a dispersion medium to promote the pelletizing property of the eurotium cristatum mycelium, so that the nutritional ingredients in the culture medium are used and converted to the maximum. Through fermentation culture in a 1L fermentation tank, the dry weight of the mycelium can be up to 15g / L. The fermentation liquor is centrifuged to collect the mycelium, then the mycelium is subjected to weight suspending by using water and then is twisted into fragments, a wall-breaking enzyme is added and uniformly mixed with the mycelium, and then the mixture is subjected to high-pressure homogenization and alcohol precipitation to extract the active ingredients in the mycelium. The preparation method improves the existing relative processes, so that the extraction rate of the active ingredients in the mycelium is improved, and large-scale production can be realized. The active ingredients in the mycelium mainly comprise polysaccharide, and have better effects on reducing fat and improving the stomach and intestines.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a preparation method of a mycelium extract of Soma coronoidis. The active ingredient of the extract is mainly polysaccharide, and has the effects of lowering blood fat and improving gastrointestinal tract. Background technique [0002] Fuzhuan brick tea is a common dark tea drink in the ethnic minority areas in the northwest frontier of my country. There is a popular saying in the local area that "tea is good and golden flowers bloom, and the tea has many flowers and good quality". Saccharomyces coronoidis is a natural probiotic bacterium that grows through the process of "flowering" under specific temperature and humidity conditions during the processing of Fuzhuan brick tea. The people in the border area mainly eat high-fat food, but the prevalence of obesity and hyperlipidemia is not high, which is closely related to the role of the metabolites of S. coronoides in tea. [0003] Fuz...

Claims

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Application Information

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IPC IPC(8): C12P19/04C08B37/00A23L1/29A23L33/00
Inventor 彭呈悦凌霄李征
Owner 湖南惟楚福瑞达生物科技有限公司
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