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Separation purifying method for DCQA (dicaffeoylquinic acid) component in jerusalem artichoke

A caffeoylquinic acid and purification method technology, which is applied in the separation/purification of carboxylic acid esters, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problems of strong toxicity and side effects, and no extraction and purification methods. Achieve the effects of easy enrichment, rich content and low production cost

Inactive Publication Date: 2010-06-23
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content of dicaffeoylquinic acid compounds in most plants is low and there are no mature extraction and purification methods. Currently, they are mainly synthesized by chemical methods. Chemically synthesized compounds have strong toxic and side effects when used in medicine. Therefore, looking for two Caffeoylquinic acid compounds are rich in plant raw materials and the establishment of a method for extracting and purifying such compounds from natural plants has great practical significance

Method used

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  • Separation purifying method for DCQA (dicaffeoylquinic acid) component in jerusalem artichoke
  • Separation purifying method for DCQA (dicaffeoylquinic acid) component in jerusalem artichoke
  • Separation purifying method for DCQA (dicaffeoylquinic acid) component in jerusalem artichoke

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Dry and crush Jerusalem artichoke leaves, weigh 40g, soak in 600ml of ethanol with a volume concentration of 70% for 12 hours, ultrasonicate for 45 minutes, filter, and save the filtrate for later use; add 520ml of ethanol with a volume concentration of 70% to soak for about 6 hours, and ultrasonically for 45 minutes , filtered; the filtrates were combined, and the filter residue was discarded. The filtrate was concentrated under reduced pressure to remove ethanol to obtain a crude extract, which was extracted with petroleum ether of the same volume as the crude extract for a total of 4 extractions. After removing the fat-soluble components, it was extracted with ethyl acetate of the same volume as the crude extract for a total of Extracted 4 times, the ethyl acetate extract was concentrated under reduced pressure to obtain an extract, which was dissolved in ethanol with a volume concentration of 20%, and then packed into a polyamide (30-60 mesh) column for chromatograph...

Embodiment 2

[0029]Dry and crush Jerusalem artichoke leaves, weigh 40g, soak in 600ml of ethanol with a volume concentration of 70% for 12 hours, ultrasonicate for 45 minutes, filter, and save the filtrate for later use; add 520ml of ethanol with a volume concentration of 70% to soak for about 6 hours, and ultrasonically for 45 minutes , filtered; the combined filtrates were discarded. The filtrate was concentrated under reduced pressure to remove ethanol to obtain a crude extract, which was extracted with petroleum ether of the same volume as the crude extract for a total of 4 extractions. After removing the fat-soluble components, it was extracted with ethyl acetate of the same volume as the crude extract for a total of Extracted 4 times, concentrated the ethyl acetate extract under reduced pressure to obtain the extract, dissolved the extract in ethanol with a volume concentration of 20%, and packed it into a column with D101 macroporous adsorption resin for separation. , 60% ethanol el...

Embodiment 3

[0031] Dry and crush Jerusalem artichoke, weigh 40g, soak in 600ml of ethanol with a volume concentration of 70% for 12 hours, ultrasonicate for 45 minutes, filter, and save the filtrate for later use; add 520ml of ethanol with a volume concentration of 70% to soak for about 6 hours, and ultrasonically for 45 minutes , filtered; the combined filtrates were discarded. The filtrate was concentrated under reduced pressure to remove ethanol to obtain a crude extract, which was extracted with petroleum ether of the same volume as the crude extract for a total of 4 extractions. After removing the fat-soluble components, it was extracted with ethyl acetate of the same volume as the crude extract for a total of Extracted 4 times, the ethyl acetate extract was concentrated under reduced pressure to obtain an extract, and after the extract was dissolved in ethanol, silica gel with the same quality as the extract was added and concentrated under reduced pressure to dryness to obtain a sam...

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Abstract

The invention discloses a separation purifying method for a DCQA (dicaffeoylquinic acid) component in jerusalem artichoke, belonging to the field of effective composition developing and utilizing. The main processing steps for extracting and separating a chemical compound in jerusalem artichoke are as follows: dried medicinal materials of jerusalem artichoke are crushed, ultrasonic or backflow extraction is carried out by ethanol (50 to 80%) in the quantity of 10 to 20 times, petroleum ether is degreased, extraction is carried out by ethyl acetate, and extraction liquid is decompressed and concentrated into an extractum. The extractum is preliminarily purified by resin or silica gel column chromatography and is further purified by SephdexLH20 so as to obtain the DCQA component with the content of higher than 85%. With simple technique and easy operation, the separation purifying method adopts diversified column chromatography to separate and purify the chemical compound of DCQA in jerusalem artichoke; the content of DCQA in a finished product is high, the source of raw materials is rich, the resin or SephdexLH20 can be recycled, and the industrialization is easy.

Description

technical field [0001] The invention relates to dicaffeoylquinic acid, in particular to a method for separating and purifying dicaffeoylquinic acid components in Jerusalem artichoke. Background technique [0002] Dicaffeoylquinic acid (Dicaffeoylquinic acid) is a phenolic acid natural compound formed by condensation of quinic acid and two molecules of caffeic acid through esterification reaction. According to different substitution positions, it can be mainly divided into 1,3-di-O -Caffeoylquinic acid, 1,4-di-O-caffeoylquinic acid, 1,5-di-O-caffeoylquinic acid, 3,4-di-O-caffeoylquinic acid, 3 , 5-di-O-caffeoylquinic acid, 4,5-di-O-caffeoylquinic acid 6 kinds. As a pharmacological active ingredient, dicaffeoylquinic acid has a wide range of effects, such as anti-oxidation, anti-inflammation, anti-liver damage, anti-microbial, anti-mutagenesis, and improving immunity. It has a certain curative effect. Studies in recent years have shown that dicaffeoylquinic acid has special...

Claims

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Application Information

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IPC IPC(8): C07C69/732C07C67/48A61K36/28
Inventor 肖红斌袁晓艳高明哲杜昱光程受衍
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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