Thin-layer chromatography discriminating method of schizomussaenda dehiscens

A technique of thin-layer chromatography and golden flower, which is applied in the field of thin-layer chromatography identification of cracked fruit golden flower, can solve the problems such as difficulty in distinguishing golden flower plants, and achieves the effects of convenient operation, fast development speed and simple equipment.

Active Publication Date: 2018-02-09
广西壮族自治区食品药品检验所
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Problems solved by technology

[0003] Plants of the genus Jadeleaf and Cinnamonia are widely distributed in Guangxi and are widely used. The plant forms of different varieties are relatively similar, and their salient features are composed of a white deformed sepal and a few golden

Method used

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  • Thin-layer chromatography discriminating method of schizomussaenda dehiscens

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Experimental program
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Effect test

Embodiment

[0015] Embodiment, content is as follows:

[0016] Reference substance: chlorogenic acid (provided by the China National Inspection Institute, batch number 110753-201415, with a content of 96.2%), 3,5- O -Dicaffeoylquinic acid (provided by the China National Inspection Institute, batch number 111782-201405, the content is calculated as 92.0%), 4,5- O - Dicaffeoylquinic acid (provided by the China Inspection Institute, batch number 111894-201102, the content is 94.1%).

[0017] The identification steps are as follows:

[0018] (1) Preparation of the test solution: Take 1 g of Cracked Golden Flower powder, add 20 mL of methanol, ultrasonically treat for 30 minutes, filter, evaporate the filtrate to dryness, add 2 mL of methanol to dissolve the residue, and use it as the test solution.

[0019] (2) Preparation of reference solution: take chlorogenic acid, 3,5- O -dicaffeoylquinic acid, 4,5- O - Dicaffeoylquinic acid reference substance, add methanol to make a mixed reference ...

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Abstract

The invention discloses a thin-layer chromatography discriminating method of schizomussaenda dehiscens. According to the invention, schizomussaenda dehiscens is taken as a main component, a mixed solution of chlorogenic acid, 3,5-O-dicaffeoylquinic acid, and 4,5-O-dicaffeoylquinic acid is taken as a reference substance solution, and a thin-layer chromatography (Chinese pharmacopoeia 2015 edition general rule 0502) is referred for testing. The method has the advantages of convenient operation, simple equipment, easy color development, and fast expansion rate, and provides basis for discriminating the schizomussaenda dehiscens.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine identification, in particular to a thin-layer chromatographic identification method for the golden flower of cracked fruit. Background technique [0002] Cracking Golden Flower (Latin scientific name: Schizomussaenda dehiscens (Craib) H.L. Li) large shrub, up to 7-8 meters; shoots are strigillose, then nearly glabrous, light brown or grayish brown. Leaves are thin and papery, oblanceolate, oblong-oblanceolate or ovate-lanceolate. Spike-shaped scorpioid cymes terminal, many flowers; flowers nearly sessile; bracts persistent or caducous, narrow; mosaic leaves ovate-lanceolate, broadly ovate or very often ovate, apex shortly pointed Or obtuse, base cuneate or acuminate; corolla lobes broadly obovate. Capsule obovate or elliptic obovate; seeds small, angular, covered with small dimples and grooves. The flowering period is from May to October, and the fruiting period is from J...

Claims

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Application Information

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IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 张涛谢培德林雀跃黄清泉吴桂凡白桂昌朱雪妍
Owner 广西壮族自治区食品药品检验所
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