Thin-layer chromatography identification method forwild chrysanthemum medicinal material and formula granule and application

A technology of formula particle and thin layer identification, which is applied in the directions of material separation, analysis of materials, preparation of test samples, etc., can solve the problems of single identification component, unable to reflect the quality and functional components of wild chrysanthemum medicinal materials as a whole, and achieve the operation Simple, good application prospect, good commercial value effect

Active Publication Date: 2020-12-11
劲牌持正堂药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the traditional identification of wild chrysanthemums, toxic reagents such as chloroform and methyl ethyl ketone are used, and mongoside and chlorogenic ac

Method used

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  • Thin-layer chromatography identification method forwild chrysanthemum medicinal material and formula granule and application
  • Thin-layer chromatography identification method forwild chrysanthemum medicinal material and formula granule and application
  • Thin-layer chromatography identification method forwild chrysanthemum medicinal material and formula granule and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A thin-layer identification method for wild chrysanthemum medicinal materials and formula granules, comprising the following steps:

[0034] a. Take chlorogenic acid, mongoside, 4,5-di-O-caffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, luteolin, luteolin reference substance, Add 80% concentration of methanol to make a solution containing 1mg per 1mL as the reference solution.

[0035] b. Add 1 g of wild chrysanthemum as reference medicinal material, add 15 ml of 80% methanol, ultrasonicate for 15 minutes, filter, and the filtrate is used as the test solution.

[0036] c. Take 1 g of the sample to be tested, add 15 ml of 80% methanol, ultrasonicate for 15 minutes, filter, and the filtrate is used as the test solution.

[0037] d. According to the thin-layer chromatography (general rule 0502) test, draw 2 μl of the test solution, 10 μl of the reference medicinal material solution, and 5 μl of the reference substance solution, respectively spot on the same high-efficienc...

Embodiment 2

[0039] The basic steps are the same as in Example 1, except that the ratio of ethyl acetate, n-butanol, formic acid and water in the developer is 10:1:1:1. Test results such as figure 2 As shown, the chromatographic separation of the test product and the reference medicinal material is poor at the corresponding positions of the chlorogenic acid and mongoside chromatograms.

Embodiment 3

[0041] Take the test sample, reference substance, and reference medicinal material solution of the wild chrysanthemum formula granules, respectively, and point them on the same silica gel G, and use ethyl acetate-n-butanol-formic acid-water as the developer in the ratio of 10:1:0.5:0.5, and develop , take it out, dry it in the air, spray it with 10% sulfuric acid ethanol solution, heat it at 70°C, and inspect it under an ultraviolet lamp (365nm). Such as image 3As shown, the substances corresponding to each label are: 1 and 2 are different batches of wild chrysanthemum formula granules; 3. Wild chrysanthemum control medicinal material; 4. Chlorogenic acid; O-caffeoylquinic acid; 7, 3,5-O-dicaffeoylquinic acid; 8, luteolin; 9, wild chrysanthemum; 10, luteolin. Compared with Example 1, the chromatograms of the test product and the reference medicinal material are not clear at the corresponding positions of the chromatograms of 4,5-di-O-caffeoylquinic acid and luteolin.

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Abstract

The invention relates to a thin-layer chromatography identification method for a wild chrysanthemum medicinal material and formula granules. The thin-layer chromatograms of the wild chrysanthemum medicinal material and formula granules are compared with the chromatograms of a wild chrysanthemum reference medicinal material as well as chlorogenic acid, linarin, 4,5-dicaffeoylquinic acid, 3, 5-O-dicaffeoylquinic acid, galuteolin and luteolin reference substance; so that effective components in the wild chrysanthemum medicinal material and formula granules can be rapidly and effectively identified. three isomers namely, chlorogenic acid, 4,5-dicaffeoylquinic acid and 3, 5-O-dicaffeoylquinic acid and two natural flavonoid compounds, namely,the galuteolin and luteolin in the wild chrysanthemumflower medicinal material and formula granules are identified by thin-layer chromatography for the first time; and the wild chrysanthemum flower medicinal material and the formula granules can be enriched, separated and purified at corresponding Rf positions of a silica gel plate by using the method. The chlorogenic acid, the isomeride component of chlorogenic acid, and the flavonoid compounds canbe prepared, so that high commercial value can be realized. The method is environmentally friendly and convenient to operate, good in repeatability and specificity and good in application prospect.

Description

technical field [0001] The invention relates to the field of quality control of traditional Chinese medicines, in particular to a thin-layer chromatography identification method and application of wild chrysanthemum medicinal materials and formula granules. Background technique [0002] Wild chrysanthemum is the dry flower head of Chrysanthemum indicum L. in Compositae. Its taste is bitter, pungent, cool, heat-clearing and detoxifying, prevention and treatment of influenza, treatment of high blood pressure, hepatitis effect is obvious. [0003] The efficacy of wild chrysanthemum is closely related to its material composition. According to literature reports, wild chrysanthemum contains chlorogenic acid, mongoside, 4,5-di-O-caffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, etc. Ninic acid, luteolin, luteolin and other components, among which 4,5-dicaffeoylquinic acid and 3,5-dicaffeoylquinic acid are isomers of chlorogenic acid. Luteolin and luteolin are a kind of natural f...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/94G01N1/28
CPCG01N30/90G01N30/94G01N1/28
Inventor 靳步昆胡辉李清安龚华梦翟红伟龙林成焕波吴新丁苗陈素娟徐凯赵玉婷黄茜茜程佩佩廖子蔚
Owner 劲牌持正堂药业有限公司
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