Method for bioconversion of chlorogenic acids in plants by adopting aspergillus aculeatus

A technology of Aspergillus aculeatus, biotransformation, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve problems such as other uses of strains not mentioned, and achieve short transformation time, strong specificity, and high value. Effect

Active Publication Date: 2013-12-04
巴州正达绿源生物科技有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

and a method for preparing 5,7,8,4'-tetrahydroxyisoflavones with the strain, but no other uses of the strain are mentioned

Method used

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  • Method for bioconversion of chlorogenic acids in plants by adopting aspergillus aculeatus
  • Method for bioconversion of chlorogenic acids in plants by adopting aspergillus aculeatus
  • Method for bioconversion of chlorogenic acids in plants by adopting aspergillus aculeatus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Use conventional Aspergillus aculeatus solid koji culture medium, add water to extract after fermentation to obtain Aspergillus aculeatus enzyme solution, add Artemisia annua extract containing 1.0% (w / v) chlorogenic acid to 100ml enzyme solution, and analyze the extract by HPLC see results figure 1 As shown, transform in a water bath at 40°C for 1 h, take the supernatant for HPLC analysis, the results are shown in figure 2 As shown, 1-caffeoylquinic acid, 3-caffeoylquinic acid, 4-caffeoylquinic acid, 4-feruloylquinic acid, 1,5-dicaffeoylquinic acid were obtained by conventional extraction methods, 1-caffeoyl-5-feruloylquinic acid, 1-feruloyl-5-caffeoylquinic acid, 3,5-dicaffeoylquinic acid and other products (see Figure 4 shown).

Embodiment 2

[0034] Aspergillus aculeatus solid koji culture medium was fermented and extracted with water to obtain Aspergillus aculeatus enzyme solution, 100ml of enzyme solution was added with 15% (w / v) chlorogenic acid wild mugwort extract, transformed in a water bath at 70°C for 60 hours, and then used conventional High-purity 3-caffeoylquinic acid, 4-caffeoylquinic acid, 4-feruloylquinic acid, 1,5-dicaffeoylquinic acid and other products were obtained by chromatography and other methods.

Embodiment 3

[0036] Use conventional Aspergillus aculeatus solid koji culture medium, after fermentation, add water to extract Aspergillus aculeatus enzyme solution, add 8% (w / v) chlorogenic acid Inula inula extract to 100ml enzyme solution, and transform in a water bath at 10°C 30h, high-purity 1-caffeoylquinic acid, 3-caffeoylquinic acid, 4-caffeoylquinic acid, 4-feruloylquinic acid, 1,5-dicaffeoylquinic acid can be obtained by recrystallization acid products.

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Abstract

The invention relates to a method for directionally converting and purifying chlorogenic acids by using aspergillus aculeatus CCTCC No.M2011264 and an enzyme liquid of the aspergillus aculeatus. The method comprises the following steps: the aspergillus aculeatus is inoculated to a liquid or solid enzyme generation culture medium, the fungus or enzyme obtained from fermentation is used for converting plant extracting solution or powder containing the chlorogenic acids. According to the invention, 1, 5-dicaffeoylquinic acid in the system of the method is reserved in the solution rather than being converted, and the solution can be purified as partial compositions are consumed during the conversion process (for example, 99% above of 4, 5-dicaffeoylquinic acid with property extremely similar to the that of the 1, 5-dicaffeoylquinic acid is consumed), so that the follow-up separation and purification steps are eliminated, and the method can be taken as a novel method for purifying the 1, 5-dicaffeoylquinic acid; besides, through controlling the conversion condition, the limitation that only caffeic acid, ferulic acid and quinic acid can be obtained by adopting the conventional conversion method is overcome.

Description

technical field [0001] The invention provides a new source for preparing 1-caffeoylquinic acid, 3-caffeoylquinic acid, 4-caffeoylquinic acid, 3-feruloylquinic acid, 4-feruloylquinic acid and a A new method for the purification of 1,5-dicaffeoylquinic acid. In particular, the method for converting chlorogenic acid with the enzyme solution of Aspergillus aculeatus belongs to the field of biotransformation of natural products. Background technique [0002] Chlorogenic acids are ester compounds formed by condensation of trans-cinnamic acids (including caffeic acid, ferulic acid, coumaric acid, sinapic acid, etc.) and quinic acid and its derivatives (M.N.Clifford, Chlorogenic acids and other cinnamates–nature, occurrence, dietary burden, absorption and metabolism, Journal of the Science of Food and Agriculture, 2000, 80:1033-1043.). Modern pharmacological studies have shown that chlorogenic acid, as a type of effective active ingredient in traditional Chinese medicine derived f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/62C12R1/66
Inventor 陈育如张伟娜马迎迎钱媛媛孙欢
Owner 巴州正达绿源生物科技有限公司
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