Method for bioconversion of chlorogenic acids in plants by adopting aspergillus aculeatus
A technology of Aspergillus aculeatus, biotransformation, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve problems such as other uses of strains not mentioned, and achieve short transformation time, strong specificity, and high value. Effect
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Embodiment 1
[0032] Use conventional Aspergillus aculeatus solid koji culture medium, add water to extract after fermentation to obtain Aspergillus aculeatus enzyme solution, add Artemisia annua extract containing 1.0% (w / v) chlorogenic acid to 100ml enzyme solution, and analyze the extract by HPLC see results figure 1 As shown, transform in a water bath at 40°C for 1 h, take the supernatant for HPLC analysis, the results are shown in figure 2 As shown, 1-caffeoylquinic acid, 3-caffeoylquinic acid, 4-caffeoylquinic acid, 4-feruloylquinic acid, 1,5-dicaffeoylquinic acid were obtained by conventional extraction methods, 1-caffeoyl-5-feruloylquinic acid, 1-feruloyl-5-caffeoylquinic acid, 3,5-dicaffeoylquinic acid and other products (see Figure 4 shown).
Embodiment 2
[0034] Aspergillus aculeatus solid koji culture medium was fermented and extracted with water to obtain Aspergillus aculeatus enzyme solution, 100ml of enzyme solution was added with 15% (w / v) chlorogenic acid wild mugwort extract, transformed in a water bath at 70°C for 60 hours, and then used conventional High-purity 3-caffeoylquinic acid, 4-caffeoylquinic acid, 4-feruloylquinic acid, 1,5-dicaffeoylquinic acid and other products were obtained by chromatography and other methods.
Embodiment 3
[0036] Use conventional Aspergillus aculeatus solid koji culture medium, after fermentation, add water to extract Aspergillus aculeatus enzyme solution, add 8% (w / v) chlorogenic acid Inula inula extract to 100ml enzyme solution, and transform in a water bath at 10°C 30h, high-purity 1-caffeoylquinic acid, 3-caffeoylquinic acid, 4-caffeoylquinic acid, 4-feruloylquinic acid, 1,5-dicaffeoylquinic acid can be obtained by recrystallization acid products.
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