Quality control method for erigeron breviscapus (Vant.) hand-mazz.

Abstract

The invention relates to the field of the quality control on traditional Chinese medicine, specifically to a quality control method for erigeron breviscapus(Vant.) hand-mazz. The method comprises a fingerprint detection method and a multiple-indicative content detection method for erigeron breviscapus (Vant.) hand-mazz, wherein the fingerprint detection method employs UPLC detection method, through which 24 characteristic peaks are found and 8 characteristic peaks are ascribed. By using content detection method for erigeron breviscapus(Vant.) hand-mazz., the UPLC-PDA method, contents of following constituents are determined: erigeroside, chlorogenic acid, breviscpini glycoside, Scutellarin, 3,4-0-dicaffeoylquinic acid, 3,5-0-dicaffeoylquinic acid and 4,5-0-dicaffeoylquinic acid. Compared with the prior art, the fingerprint detection method and the multiple-indicative content detection method for erigeron breviscapus (Vant.) hand-mazz are improved in the invention, so that the quality of erigeron breviscapus (Vant.) hand-mazz. can be controlled in a convenient, rapid and accurate manner, thereby ensuring the quality of erigeron breviscapus (Vant.) hand-mazz. stable, uniform and controllable.

Description

technical field [0001] The invention relates to the field of quality control of traditional Chinese medicines, in particular to a method for quality control of breviscapine medicinal material. Background technique [0002] The quality control and guarantee of traditional Chinese medicine is the core issue in the modernization, industrialization and internationalization of traditional Chinese medicine. The diversity and complexity of the chemical components of traditional Chinese medicine is the material basis for exerting its curative effect, and it is also the difficulty and focus of quality evaluation. The traditional method of quality control using a single component as an indicator is increasingly unable to meet the requirements of the current quality control of traditional Chinese medicine. In the process of modernization of traditional Chinese medicine, the fingerprint of traditional Chinese medicine, which has two basic characteristics of integrity and fuzziness, has...

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Problems solved by technology

However, HPLC is often used to determine the content of its main active ingredient, scutellarin, in the existing medicinal materials of Erigeron breviscapus (Liu Peng, Zhou Jun, Qin Xiangyang, Journal of Fourth Military Medical University, 2006, 27 (9): 781; Lin Yanzhi, Chen Xiaohui, Tang Xing, Journal of Pharmaceutical Analysis, 2005, 25(5): 594; Yang Wenwen, Zhang Yi, Duan Junguo, Chengdu University of Traditional Chinese Medicine, 2001, 24(3): 37), there is only one document reporting the The determination of 3,5-biscaffeoyloxyquinic acid in Asarum medicinal materials (Yang Lihui, Zhang Weidong, Li Tingzhao, Chinese Journal of Preventive Medicine, 2005, 6 (3): 216), failed to control its contained The components of flavonoids, caffeic acid esters and phenolic acids cannot fully reflect their quality; the HPLC fingerprint of Erigeron breviscapus established in the research is not only poor in separation effect but also takes too long to analyze because of its complex chemical components, which often takes 60min~ 90min (Gao Zhan, Huang Luosheng, Strait Pharmacy, 2005, 17(3): 86; Zhang Ping, Clinician of Chinese and Foreign Health Abstracts, 2008, 5(7): 80; Zhang Xu, Geng Boxian, Chen Lingling, China Journal of Pharmaceutical Industry, 2003 , 34(6):270)

At present, in the field of analysis, there is no relevant literature report on the use of UPLC to establish the fingerprint of Erigeron breviscapus or the determination of multi-index components.

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