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Construction method of wild chrysanthemum flower UPLC (Ultra Performance Liquid Chromatography) fingerprint spectrum

A technology of fingerprint spectrum and construction method, applied in the field of fingerprint spectrum construction, can solve the problems of low detection accuracy and sensitivity, long detection time, uneven quality standard control of wild chrysanthemum, etc. Sensitivity, saving detection time, and the effect of simple detection method

Inactive Publication Date: 2014-07-16
GUANGZHOU XINGQUN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wild chrysanthemum can be widely used to treat boils, carbuncles, sore throat, red eyes due to wind-fire, headache and dizziness, etc. Quality standard control is uneven
Fingerprints are used as a detection method to control the quality uniformity and stability of traditional Chinese medicines, but at present, the time required for traditional HPLC fingerprint detection of wild chrysanthemum medicinal materials is too long, the required sample volume is large, and the detection accuracy and sensitivity are not high.

Method used

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  • Construction method of wild chrysanthemum flower UPLC (Ultra Performance Liquid Chromatography) fingerprint spectrum
  • Construction method of wild chrysanthemum flower UPLC (Ultra Performance Liquid Chromatography) fingerprint spectrum
  • Construction method of wild chrysanthemum flower UPLC (Ultra Performance Liquid Chromatography) fingerprint spectrum

Examples

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Effect test

Embodiment 1

[0039] The present embodiment provides a kind of assay method of Flos Chrysanthemi UPLC fingerprint, comprises the steps:

[0040] (1) Preparation of the test solution: take the wild chrysanthemum medicinal material, crush it through a 60-mesh sieve, take about 0.5g of the sample powder, weigh it accurately, place it in a 100mL conical flask, add 50mL of methanol, weigh it, and use an ultrasonic power of 160W , frequency 59kHz, 30min, cooling and replenishing, filtering through a 0.22μm microporous membrane, taking the subsequent filtrate to obtain the test solution;

[0041] (2) Preparation of the reference solution: Accurately weigh the appropriate amount of chlorogenic acid, luteolin, luteolin and mongoside, dissolve it in 75% methanol solution and set the volume to 100mL, and obtain concentrations of 0.0598, 0.1129, 0.0565 and 0.1965 μg / μL reference substance single solution, respectively accurately draw 10mL of each reference substance solution and put it in a 50mL volume...

Embodiment 2

[0045] The present embodiment provides a kind of assay method of Flos Chrysanthemi UPLC fingerprint, comprises the steps:

[0046] (1) Preparation of the test solution: take the wild chrysanthemum medicinal material, crush it through a 60-mesh sieve, take about 0.5g of the sample powder, weigh it accurately, place it in a 100mL conical flask, add 55mL of methanol, weigh it, and use an ultrasonic power of 170W , frequency 60kHz, 25min, cooling and replenishing, 0.2μm microporous membrane filtration, take the subsequent filtrate to obtain the test solution;

[0047] (2) Preparation of the reference substance solution: Accurately weigh an appropriate amount of chlorogenic acid, luteolin, luteolin and mongoside, dissolve it in 70% methanol solution and set the volume to 100mL to obtain a single solution of the reference substance, and accurately draw each Put 10mL of the reference substance solution in a 50mL volumetric flask, and mix the reference substance solution at constant v...

Embodiment 3

[0051] The present embodiment provides a kind of assay method of Flos Chrysanthemi UPLC fingerprint, comprises the steps:

[0052] (1) Preparation of the test solution: Take the wild chrysanthemum medicinal material, crush it and pass through a 60-mesh sieve, take about 0.5g of the sample powder, weigh it accurately, place it in a 100mL conical flask, add 45mL of methanol, weigh it, and use an ultrasonic power of 150W , frequency 55kHz, 35min, cooling and replenishing weight, 0.25μm microporous membrane filtration, take the subsequent filtrate to obtain the test solution;

[0053] (2) Preparation of reference substance solution: Accurately weigh an appropriate amount of chlorogenic acid, luteolin, luteolin and mongoside, dissolve it in 80% methanol solution and set the volume to 100mL to obtain a single solution of reference substance, and accurately draw each Put 10mL of the reference substance solution in a 50mL volumetric flask, and mix the reference substance solution at c...

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Abstract

The invention discloses a construction method of a wild chrysanthemum flower UPLC fingerprint spectrum. The method comprises the following steps: crushing a wild chrysanthemum flower medicinal material, precisely weighing, adding methanol, performing ultrasonic treatment after weighing, filtering by use of a microfiltration membrane, and taking subsequent filtrate to obtain a test sample solution; respectively taking chlorogenic acid, galuteolin, luteolin and linarin, precisely weighing, and dissolving by use of the methanol to obtain a reference solution; and respectively measuring the test sample solution and the reference solution by use of an ultrahigh-efficiency liquid chromatogram system to generate chromatogram peaks of the test sample solution and the reference solution so as to generate the wild chrysanthemum flower UPLC fingerprint spectrum. The construction method of the wild chrysanthemum flower UPLC fingerprint spectrum related to the invention is simple, easy to operate and greatly improved in spectrum analytic capability, and has the advantages of improving the accuracy of results and greatly improving the stability and sensitivity of the detection of the fingerprint spectrum.

Description

technical field [0001] The invention relates to the field of fingerprint atlas construction, in particular to a method for constructing UPLC fingerprint atlas of wild chrysanthemum. Background technique [0002] At present, Chinese herbal medicine plays a huge role in the field of traditional Chinese medicine treatment, and Chinese herbal medicine, especially some Chinese medicine granules, is convenient in the process of taking. The wild chrysanthemum is the flower head of the perennial herb of the Compositae family, which is similar to the chrysanthemum. It grows wild on hillside grasslands and beside roadsides. It is better to be yellow, sessile, complete, fragrant, and not fully bloomed. Wild chrysanthemum can be widely used to treat boils, carbuncles, sore throat, red eyes due to wind-fire, headache and dizziness, etc. Quality standard control is uneven. Fingerprints are used as a detection method to control the quality uniformity and stability of traditional Chinese...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/86
Inventor 姚江雄许招懂蒋莉娟方铁铮黎志坚林丽美
Owner GUANGZHOU XINGQUN PHARMA
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