Method for extracting luteolin and beta-sitosterol from peanut shells

A technology of luteolin and peanut shells, applied in the directions of steroids, organic chemistry, etc., can solve the problems of difficult impurity removal, complicated process and high cost, and achieves the advantages of reducing the risk of environmental pollution, simple refining method and low production cost. Effect

Active Publication Date: 2015-06-17
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0019] In summary, there are many preparation methods for luteolin and β-sitosterol. The chemical synthesis method is complex and costly. Due to the introduction of catalysts and other components and by-products, the removal of impurities is very difficult, and the product Security becomes an important issue

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The collected peanut shells were dried in the sun, pulverized to 20-40 mesh powder, 100 kg of peanut shell powder was taken, 400 L of 75% ethanol aqueous solution was added, heated to 45 °C for reflux extraction for 3 hours, filtered with a 0.22 μm ceramic membrane once to obtain the filtrate, and the filter residue was filtered with 75 200L of % ethanol aqueous solution was extracted once, and 530L of filtrate was combined; 6kg of granular activated carbon was added to the filtrate for decolorization, and the 0.22 μm ceramic membrane was filtered to obtain a decolorized filtrate; the decolorized filtrate was concentrated under reduced pressure at 40 to 50°C and below -88kPa To dryness, 9.45kg of dry matter was obtained, and then 15L of aqueous sodium hydroxide solution with a mass ratio of 10% was added, mixed evenly, and allowed to stand for 25min; after standing, 40L of ethyl acetate was added to the solution for extraction to obtain a solution containing ethyl acetate...

Embodiment 2

[0048] The collected peanut shells were dried in the sun, pulverized to 20-40 mesh powder, 200 kg of peanut shell powder was taken, 600 L of 70% ethanol aqueous solution was added, heated to 50 °C for reflux extraction for 2.5 h, filtered with a 0.22 μm ceramic membrane once to obtain the filtrate, and the filter residue was used 300L of 70% ethanol aqueous solution was extracted once, and 820L of filtrate was combined; 20kg of granular activated carbon was added to the filtrate for decolorization, and a 0.22 μm ceramic membrane was filtered to obtain a decolorized filtrate; the decolorized filtrate was decompressed at 40 to 50°C and below -88kPa Concentrate to dryness to obtain 21.5kg of dry matter, then add 30L of aqueous sodium hydroxide solution with a mass ratio of 11%, mix evenly, and let stand for 20-30min; after standing, add 40L of ethyl acetate to the solution for extraction, and obtain a solution containing acetic acid. The extract of ethyl ester and the aqueous solu...

Embodiment 3

[0050]The collected peanut shells are sun-dried, pulverized to 20-40 mesh powder, take 300 kg of peanut shell powder, add 1000 L of 80% ethanol aqueous solution, heat to 40 °C for reflux extraction for 2-5 h, filter with a 0.22 μm ceramic membrane once to obtain filtrate, and filter residues Repeat the extraction once with 400 L of 80% ethanol aqueous solution, and combine 1300 L of filtrate; add 30 kg of granular activated carbon to the filtrate for decolorization, and filter with a 0.22 μm ceramic membrane to obtain a decolorized filtrate; The decolorized filtrate is concentrated under reduced pressure at 45 ° C and below -88 kPa To dryness, 31.8 kg of dry matter was obtained, and then 50 L of aqueous sodium hydroxide solution with a mass ratio of 8% was added, and the mixture was uniformly mixed and allowed to stand for 20 min; after standing, 150 L of ethyl acetate was added to the solution for extraction to obtain a solution containing ethyl acetate. The extract and the aq...

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Abstract

The invention relates to a method for extracting luteolin and beta-sitosterol from peanut shells. The method comprises the following steps: taking the peanut shells as materials, cleaning the peanut shells to remove impurities, crushing the cleaned peanut shells, performing extraction on the crushed peanut shells by an ethanol solution, carrying out filtration, decolorization and concentration under reduced pressure to obtain a luteolin and beta-sitosterol mixture, then mixing the luteolin and beta-sitosterol mixture with an alkali liquor, after that, carrying out extraction separation to obtain beta-sitosterol, performing extraction after pH adjustment to obtain a luteolin crude product, and performing alcohol dissolution, crystallization and recrystallization on the crude product to obtain a luteolin product with relatively high purity. The method is simple to operate, demands less on equipment, lowers the manufacturing cost of luteolin and reduces the risk of environmental pollution.

Description

technical field [0001] The invention relates to a method for extracting luteolin and beta-sitosterol by utilizing peanut shells, and belongs to the technical field of natural product chemistry. Background technique [0002] Peanut is the second largest edible vegetable oil source and protein resource in my country. Its waste peanut shell contains a small amount of lipids, mainly luteolin and β-sitosterol, which have been used as raw materials for luteolin extraction, and have been fully obtained. use. Luteolin has the strongest antioxidant activity, and its antioxidant properties can be used to inhibit the failure of oily foods. The results of pharmacology and clinical experiments show that luteolin has antibacterial, antiviral, lowering blood lipid and cholesterol, and preventing cardiovascular diseases in vivo. In recent years, a large number of domestic and foreign literatures have reported the antitumor activity of luteolin. It has a significant inhibitory effect on a v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07D311/40C07J9/00
CPCC07D311/30C07D311/40C07J9/00
Inventor 向华
Owner HUNAN AGRICULTURAL UNIV
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