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Method for separating and purifying luteolin

A luteolin and purpose technology, which is applied in the field of separation and purification of active ingredients of natural products, can solve the problems of large consumption of mobile phase, large amount of solvents, high operating costs, etc., achieve high strength and stability, avoid large consumption, and reduce separation costs Effect

Inactive Publication Date: 2010-05-26
NORTH CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extraction products obtained by the above-mentioned methods are total flavonoids, and it is impossible to obtain luteolin with higher purity.
At present, the methods for further separation and purification of luteolin include high performance liquid chromatography and chromatography. Although these two methods have high separation efficiency, the liquid chromatography equipment required by liquid chromatography is expensive and the extraction amount is limited. Chromatographically pure mobile phase consumes a lot of money and has high operating costs
Chromatographic silica gel cannot be reused and requires a large amount of solvent, so the separation cost is also high

Method used

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  • Method for separating and purifying luteolin
  • Method for separating and purifying luteolin
  • Method for separating and purifying luteolin

Examples

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Effect test

example 1

[0032] 1. Preparation of luteolin molecularly imprinted polymer

[0033] Weigh 0.731g luteolin, 0.706g acrylamide, 10g pentaerythritol triacrylate (PETRA) and 0.03g azobisisoheptanonitrile and dissolve them in 20ml acetone, sonicate in a beaker, then pour the solution into a sealable container (test tube), seal it, pass Ar 2 For deoxidation, put the sealed container into a constant temperature water bath at 60°C for polymerization. The preparation of blank polymers (NMIPs) was the same except that the template molecule luteolin was not added. After the polymerization is completed, the bulk polymer in the container is smashed into small pieces and ground to obtain granular polymer. A solution of methanol: acetic acid (9: 1) was used to elute to remove solvents, initiators and template molecules that did not participate in the polymerization to obtain a molecularly imprinted polymer of luteolin.

[0034] 2. Separation and purification of luteolin concentrate

[0035] Weigh 3...

example 2

[0037] 1. Preparation of luteolin molecularly imprinted polymer

[0038] Weigh 0.713g luteolin, 0.883g 2,6-diacrylamide pyridine, 10g EDMA and 0.03g AIBN and dissolve in 20ml acetone, ultrasonically in a beaker, then pour the solution into a sealable container (round bottom flask) In, to seal, through Ar 2 For deoxidation, put the sealed container into a constant temperature water bath at 60°C for polymerization. The preparation of blank polymers (NMIPs) was the same except that the template molecule luteolin was not added. After the polymerization is completed, the bulk polymer in the container is smashed into small pieces and ground to obtain granular polymer. A solution of methanol: acetic acid (9: 1) was used to elute to remove solvents, initiators and template molecules that did not participate in the polymerization to obtain a molecularly imprinted polymer of luteolin.

[0039] 2. Separation and purification of luteolin concentrate

[0040] Weigh 2 g of the eluted lu...

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Abstract

The invention aims to provide a material for preparing a molecular imprinted polymer by taking luteolin as a template and adopting a molecular imprinting technique method, and a method for separating and purifying the luteolin in a natural product, and belongs to the field of separation and purification of effective components from the natural product. The method comprises the following steps: (1) crude extraction, namely sequentially washing, drying, crushing and sieving peanut shells, extracting the peanut shell powder with 70 percent ethanol time by time under the assistance of ultrasonic waves, and concentrating and drying the extract; (2) preparation of the polymer, namely weighing the pure luteolin, a functional monomer, a cross-linking agent and an initiating agent in proportion, dissolving the materials in a solvent, performing ultrasonic processing, degassing and polymerization on the solution to obtain the block polymer, and sequentially grinding, sieving and eluting the block polymer to obtain the molecular imprinted polymer of the luteolin; and (3) purification, namely loading the prepared molecular imprinted polymer into a solid-phase extraction column, loading the crude extract, washing off the luteolin left on the column after removing impurities by eluting, concentrating and drying the luteolin to obtain the pure product of the luteolin.

Description

Technical field: [0001] The patent of the present invention relates to the separation and purification of luteolin in natural plants, and belongs to the field of separation and purification of active ingredients of natural products. [0002] Luteolin is a very representative natural flavonoid, which belongs to weakly acidic tetrahydroxyflavonoids. It is widely distributed in the plant kingdom and has high biological activity. [0003] Luteolin has a strong antioxidant activity, and has antibacterial, antiviral, and blood lipid and cholesterol-lowering effects in the body; luteolin is also an anti-tumor initiation factor, and studies have confirmed that it has the effect on a variety of solid tumors, ascites cancer, and leukemia cells. It has a significant inhibitory effect; luteolin can also sensitize a variety of apoptotic factors, and has the potential of multi-target anti-tumor. [0004] Inflammation plays an important role in many neurodegenerative diseases. Studies have ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30A61P31/00A61P31/12A61P3/06A61P35/00A61P25/00A61P25/28A61K36/48A61K131/00
Inventor 李红霞肖淑娟于守武
Owner NORTH CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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