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Method for determining content of scutelloside in throat-clearing granule by using high-performance liquid chromatography

A high-performance liquid chromatography technology for clearing and relieving throat, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as unfavorable drug quality, achieve good reproducibility, simple method, accurate and reliable results

Inactive Publication Date: 2016-09-07
WUZHOU INST FOR FOOD & DRUG CONTROL
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AI Technical Summary

Problems solved by technology

Its quality standard is recorded in: "Chinese Pharmacopoeia" 2005 edition, only the content determination index of baicalin, which is not conducive to controlling the quality of the drug

Method used

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Experimental program
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Embodiment 1

[0021] A method for HPLC determination of baicalin content in Qinghou Liyan Granules provided by the invention, the method is as follows:

[0022] A method for measuring the content of baicalin in Qinghou Liyan Granules by HPLC, comprising the following steps in turn:

[0023] 1) Take 5g of this product powder, add N, N-dimethylformamide and 30ml of ethanol at a volume ratio of 1:1 to dissolve, centrifuge to take the supernatant, pass it through the D101 resin column that has been treated, and first wash it with 70% ethanol Wash, collect the eluent, evaporate to dryness, then elute with 5ml of 40% ethanol, collect the eluent, evaporate to dryness, dissolve with 5ml of methanol, and filter with 0.22 μm microporous membrane as the test sample;

[0024] 2) Take 5 g of the reference substance of baicalin, dissolve it by ultrasonication for 30 minutes, make up 10 ml, and filter it with a 0.22 μm microporous membrane to obtain a stock solution of the reference substance; draw 10 ml ...

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Abstract

The invention provides a method for determining the content of scutelloside in a throat-clearing granule by using high-performance liquid chromatography. The objective of the invention is to provide the method with the advantage of high analysis efficiency, and the method can be used as a quality control method for the throat-clearing granule. The method comprises the following steps: preparing a test solution; preparing a reference substance; and carrying out specific tests, precisely sucking 2 [mu]L of a reference substance solution, 2 [mu]L of the test solution and 2 [mu]L of a negative control solution, separately injecting the solutions into a liquid chromatograph, and carrying out detection, wherein chromatographic conditions are that a used chromatographic column is an Inertsil ODS-SP liquid chromatographic column, 5 [mu]m, 4.6*250 mm; a mobile phase is acetonitrile-0.5% acetic acid in a volume ratio of 44: 56; flow velocity is 0.8 mL / min; wavelength is 287 nm; and column temperature is 30 DEG C. The method belongs to the technical field of chemical detection.

Description

technical field [0001] The invention provides a method for identifying baicalin, specifically, a method for determining the content of baicalin in Qinghou Liyan granules by HPLC; it belongs to the technical field of chemical detection. Background technique [0002] Qinghou Liyan Granules are composed of 13 traditional Chinese medicines, including Scutellaria baicalensis, Xiqingguo, Platycodon grandiflorum, Zhuru, Fructus aurantii, Panda Hai, and Juhong. Efficacy Clearing heat and throat, widening chest and moistening throat. It is used for dry throat and hoarseness caused by exogenous wind-heat; for acute and chronic pharyngitis and tonsillitis with the above syndromes, it is often used to protect the vocal cords. Its quality standards are recorded in: "Chinese Pharmacopoeia" 2005 edition one, only the content determination index of baicalin, which is not conducive to the quality control of the drug. Contents of the invention [0003] In view of the above deficiencies, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/027
Inventor 陈学松林冬杰李亚梁慧敏李澄
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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