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Method for extracting scutelloside and scutellarin from baikal skullcap root

A technology of baicalin and baicalein, which is applied in the field of extraction of baicalin and baicalein, can solve the problems of difficulty in realizing large-scale industrial production, low product purity, and high equipment requirements, so as to avoid the loss of raw materials and energy consumption, and the products The effect of high purity and fast separation speed

Inactive Publication Date: 2014-05-07
HUNAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods all have the following problems: one is that the product purity is not high, and the purified product is often a mixture of several or even dozens of compounds; Three methods, multiple separations, and complicated steps; third, high equipment requirements, high cost, difficult to scale up, and difficult to achieve large-scale industrial production
In the prior art, there is no process for the simultaneous non-column chromatographic separation and purification of baicalin and baicalein from the Scutellaria baicalensis extract

Method used

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  • Method for extracting scutelloside and scutellarin from baikal skullcap root

Examples

Experimental program
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Effect test

Embodiment 1

[0020] This embodiment includes the following steps:

[0021] (1) Solvent ultrasonic extraction: Weigh 1.0 g of Scutellaria baicalensis root powder, add 100 ml of extraction solvent, add formic acid, adjust the pH to 5, perform ultrasonic extraction for 30 min, and centrifuge at 10,000 rpm for 5 min to obtain the extract;

[0022] The extraction solvent is a mixture of acetonitrile and water, wherein the volume ratio of acetonitrile and water is 4:1;

[0023] (2) Fe 3 o 4 Magnetic nanoparticle adsorption: add 3.0 g Fe to the extract obtained in step (1) 3 o 4 Magnetic nanoparticles, stirred, ultrasonically dispersed for 2 min, shaken on a constant temperature oscillator at 25°C for 20 min, separated by a magnet, so that the solution was divided into upper and lower phases, and baicalin and baicalein were selectively adsorbed on the Fe in the lower layer. 3 o 4 Nanoparticle surface;

[0024] (3) Desorption: blot the supernatant, and remove the Fe 3 o 4 Add 100 ml of bai...

Embodiment 2

[0031] This embodiment includes the following steps:

[0032] (1) Solvent ultrasonic extraction: Weigh 5.0 g of Scutellaria baicalensis root powder, add 800 ml of extraction solvent, add acetic acid, adjust the pH to 4, ultrasonically extract for 30 min, and centrifuge at 10,000 rpm for 5 min to obtain the supernatant; the extraction solvent is The mixture of methanol and water, the volume ratio of methanol and water is 3: 1;

[0033] (2) Fe 3 o 4 Magnetic nanoparticle adsorption: Add 20.0 g Fe to the supernatant obtained in step (1) 3 o 4 Magnetic nanoparticles, stirred, ultrasonically dispersed for 2 min, shaken on a constant temperature oscillator at 25°C for 20 min, separated by a magnet, so that the solution was divided into upper and lower phases, and baicalin and baicalein were selectively adsorbed on the Fe in the lower layer. 3 o 4 Nanoparticle surface;

[0034] (3) Desorption: blot the supernatant, and remove the Fe 3 o 4 Add 800 ml of baicalin eluent to the ...

Embodiment 3

[0038] This embodiment includes the following steps:

[0039] (1) Solvent ultrasonic extraction: Weigh 10.0 g of Scutellaria baicalensis root powder, add 2000 ml of extraction solvent, then add formic acid, adjust the pH to 3, ultrasonically extract for 30 min, and centrifuge at 10000 rpm for 5 min to obtain the supernatant; the extraction solvent It is a mixture of acetone and water, the volume ratio of acetone and water is 4: 1;

[0040] (2) Fe 3 o 4 Magnetic nanoparticles adsorption: add 40 g Fe to the supernatant obtained in step (1) 3 o 4 Magnetic nanoparticles, stirred, ultrasonically dispersed for 2 min, shaken on a constant temperature oscillator at 25°C for 20 min, separated by a magnet, so that the solution was divided into upper and lower phases, and baicalin and baicalein were selectively adsorbed on the Fe in the lower layer. 3 o 4 Nanoparticle surfaces;

[0041] (3) Desorption: blot the supernatant, and remove the Fe 3 o 4 Add 2000 ml of baicalin eluent t...

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Abstract

The invention discloses a method for extracting scutelloside and scutellarin from baikal skullcap root. The method comprises the following steps of: (1) performing ultrasonic extraction on a solvent; (2) adsorbing Fe3O4 magnetic nanoparticles; and (3) performing desorption. The method is simple and rapid; separation of an absorption carrier and a feed liquid can be realized by additionally applying a magnetic field; scutelloside and scutellarin can be eluted selectively through different eluents, so that loss of raw materials and energy consumption is avoided, and cost is reduced effectively; and Fe3O4 magnetic nanoparticles can be used circularly, so that cost can be saved effectively.

Description

technical field [0001] The invention relates to a method for extracting baicalin and baicalein, in particular to a method for separating and purifying baicalin and baicalein from roots of Scutellaria baicalensis by non-column chromatography. Background technique [0002] Scutellaria baicalensis is one of the commonly used traditional Chinese medicines, which has the functions of clearing away heat and dampness, purging fire and detoxification, stopping bleeding and preventing miscarriage. The main components of Scutellaria baicalensis are flavonoids such as baicalin, baicalein, wogonin and wogonin. Among them, baicalin and baicalein are the main quality control index components of Scutellaria baicalensis and its preparations. Modern pharmacological studies have proved that baicalin and baicalin Baicalein not only has obvious antibacterial, anti-inflammatory, anti-allergic, anti-oxidative, anti-cancer and anti-viral effects, but also has certain effects on the treatment of ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08C07D311/30C07D311/40
Inventor 陈波张松张军安许光明周鹏李媛
Owner HUNAN NORMAL UNIVERSITY
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