Method for simultaneously separating wogonin and baicalein monomers from scutellaria baicalensis

A technology for simultaneous separation of wogonin and wogonin is applied in the field of simultaneous separation of wogonin and baicalein monomer, which can solve the problems of increased difficulty, low content, limited acid hydrolysis transfer rate, etc. The effect of good clinical application value

Inactive Publication Date: 2014-02-05
TIANJIN SHILAN TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low content of baicalein and wogonin in plants (generally 0.05-0.5%), it is difficult to separate directly; acid hydrolysis method can convert part of baicalin into its aglycone, but because flavonoid glycosides are 7- 0-Glucuronic acid-binding glycoside, the glycosidic bond is relatively strong and difficult to break, so the transfer rate of acid hydrolysis is limited to a certain extent; the degradation conditions of endogenous enzyme method are relatively mild, but it takes a long time, and silica gel column

Method used

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  • Method for simultaneously separating wogonin and baicalein monomers from scutellaria baicalensis
  • Method for simultaneously separating wogonin and baicalein monomers from scutellaria baicalensis
  • Method for simultaneously separating wogonin and baicalein monomers from scutellaria baicalensis

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Experimental program
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Effect test

Embodiment 1

[0028] A method for simultaneously separating wogonin and baicalein monomer from scutellaria includes the following steps:

[0029] (1) Crush 500g of Scutellaria baicalensis Georgi, add distilled water 6 times the weight of the medicinal material and 5g β-D-glucuronidase (edible enzyme), control the temperature at 50℃, and enzymatically hydrolyze for 3 hours;

[0030] (2) Filter the distilled water, put the scutellaria baicalensis material into the supercritical extraction kettle, set the extraction pressure to 20Mpa, temperature to 50°C, extraction time to 3.5 hours, entrainer ethanol, and 200ml dosage after preheating;

[0031] (3) Collected in sections, concentrated the ethanol solution and recrystallized with ethyl acetate to obtain 25.18g of baicalein and 13.64g of wogonin. The purity of baicalein was 98.23% and the purity of wogonin was 98.35 by HPLC. %.

[0032] Identification and content determination of wogonin and baicalein obtained above:

[0033] 1. Detection method

[0034]...

Embodiment 2

[0055] A method for simultaneously separating wogonin and baicalein monomer from scutellaria includes the following steps:

[0056] (1) Crush 500g of Scutellaria baicalensis Georgi, add distilled water 3 times the weight of the medicinal material and 0.05g snail enzyme (edible enzyme), control the temperature at 60℃, and enzymolyse for 10 hours;

[0057] (2) The distilled water was filtered, and the Scutellaria baicalensis was placed in a supercritical extraction kettle. After preheating, the extraction pressure was set to 35Mpa, the temperature was 40°C, the extraction time was 5 hours, the entrainer was acetone, and the amount was 25ml;

[0058] (3) Collected in sections, concentrated the acetone solution and recrystallized with petroleum ether to obtain 10g baicalein and 1.5g wogonin, respectively. The purity of baicalein was 98.27% and the purity of wogonin was 98.65% by HPLC.

Embodiment 3

[0060] A method for simultaneously separating wogonin and baicalein monomer from scutellaria includes the following steps:

[0061] (1) Crush 500g of Scutellaria baicalensis Georgi, add distilled water 15 times the weight of the medicinal material and 50g cellulase (edible enzyme), control the temperature at 35℃, and enzymatically hydrolyze for 1 hour;

[0062] (2) The distilled water was filtered, and the Scutellaria baicalensis material was placed in a supercritical extraction kettle. After preheating, the extraction pressure was set to 10Mpa, the temperature was 60°C, the extraction time was 1 hour, the entrainer was ethyl acetate, and the amount was 500ml;

[0063] (3) Collected in sections, concentrated ethyl acetate and recrystallized with acetone to obtain 10.5g of baicalein and 1.3g of wogonin, respectively. The purity of baicalein was 98.45% and the purity of wogonin was 98.29% by HPLC. .

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Abstract

The invention provides a method for simultaneously separating wogonin and baicalein monomers from scutellaria baicalensis. The method comprises the steps of crushing a scutellaria baicalensis medicinal material, adding a proper amount of water and edible enzyme to carry out enzymolysis, supercritically extracting the medicinal material subjected to the enzymolysis, fractionally collecting the medicinal material by using an entrainer, and re-crystallizing to respectively obtain baicalein and wogonin. By using the method, baicalein compound monomers can be simultaneously extracted from the scutellaria baicalensis medicinal material, the yield is increased by over 3 times than the yield of wogonin and baicalein monomers obtained by using a direct separation method, and the purities of the prepared wogonin and baicalein monomers are both over 98%; moreover, the method is simple and convenient in flow, environment-friendly, very important in production practical significance, and suitable for large-scale industrial production.

Description

Technical field [0001] The invention relates to the field of separation of natural active substances, in particular to a method for simultaneously separating wogonin and baicalein monomers from scutellaria. Background technique [0002] Scutellaria baicalensis is the dried root of Scutellaria baicalensis Georgi, a plant in the Lamiaceae family. It has the functions of clearing away heat and dampness, purging fire and detoxification, hemostasis, and anti-fetus. The main components of scutellaria are flavonoids such as Baicalin, Wogonside, Oroxylin and a small amount of Chrysin. Drug metabolism studies have found that the main components absorbed into the blood by the extract of Scutellaria baicalensis are baicalein and wogonin and a small amount of lamellarin. It is difficult for baicalin and wogonin to be absorbed by the intestine, and the bioavailability is extremely low. In recent years, it has been confirmed that wogonin has a clear anti-tumor effect. For example, wogonin can...

Claims

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Application Information

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IPC IPC(8): C12P17/06
CPCY02P20/54
Inventor 颜世利
Owner TIANJIN SHILAN TECH CO LTD
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