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Scullcap total-flavonoid aglycone extract, preparation method and use thereof

A total flavonoid aglycone and extract technology, applied in the field of medicine, can solve the problems of hidden danger of clinical drug use, high cost, and it is difficult to tell whether it is a glycoside or aglycone, etc., and achieves the improvement of renal function damage, the process is simple, and the anti-kidney resistance is achieved. The effect of interstitial fibrosis

Active Publication Date: 2009-06-17
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has great disadvantages. Due to the relatively high polarity of absolute ethanol, some glycosides with low polarity will also be extracted at the same time, and it is difficult to tell whether the experimental results of the efficacy of the extract are The role of glycosides or aglycones
It has also been reported that microbial enzymes are used to enzymatically hydrolyze glycosides in Scutellaria baicalensis plants. Compared with using enzymes in plants, this method is more expensive, and because microbial enzymes are used, microbial metabolites may be brought in. In the future, It may bring certain hidden dangers to clinical use

Method used

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  • Scullcap total-flavonoid aglycone extract, preparation method and use thereof
  • Scullcap total-flavonoid aglycone extract, preparation method and use thereof
  • Scullcap total-flavonoid aglycone extract, preparation method and use thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0018] Take the dried Scutellaria baicalensis roots, crush them, pass through a No. 1 sieve, take 1000 grams of powder, add 1 L of water and stir evenly, heat and enzymolyze it at 32°C for 5 hours, dry it in vacuum at 60°C for 16 hours, put the dry powder in In a Soxhlet extractor, 2000ml of ethyl acetate was continuously extracted for 6 hours, and the extract was decompressed to recover the solvent. That is, the extract of total flavonoid aglycone of Scutellaria baicalensis is obtained.

[0019] The characteristics of the extract: the extract is yellow, the weight is about 65g, and the yield of total flavone aglycone is 6.5%. With baicalein as the reference substance, the total flavonoid content of the extract is determined to be >45% by ultraviolet spectrophotometry.

Embodiment 2

[0021] Take the dried Scutellaria baicalensis roots, crush them, pass through a No. 1 sieve, take 1000 grams of powder, add 3 L of water and stir evenly, heat and enzymolyze at 32°C for 9 hours, vacuum dry at 60°C for 16 hours, and place the dry powder in In the Soxhlet extractor, 5000ml of ethyl acetate was continuously extracted for 8 hours, and the extract was recovered under reduced pressure to recover the solvent. That is, the extract of total flavonoid aglycone of Scutellaria baicalensis is obtained.

[0022] The characteristics of the extract: the extract is yellow, the weight is about 115g, and the yield of total flavonoid aglycone is 11.5%. The total flavonoid aglycone content of the extract is determined to be >50% by ultraviolet spectrophotometry.

Embodiment 3

[0024] Take the dried Scutellaria baicalensis roots, crush them, pass through a No. 1 sieve, take 1,000 grams of powder, add 5 L of water, stir evenly, keep warm at 32°C for 7 hours, bake at 60°C for 16 hours, put the dry powder on the cable In the extractor, 8000ml of ethyl acetate was continuously extracted for 10 hours, and the extract was recovered under reduced pressure to recover the solvent. That is, the extract of total flavonoid aglycone of Scutellaria baicalensis is obtained.

[0025] The characteristics of the extract: the extract is yellow, the weight is about 120g, and the yield of total flavonoid aglycone is 12.0%. The total flavonoid aglycone content of the extract is determined to be >60% by ultraviolet spectrophotometry.

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Abstract

The invention discloses a radix scutellariae total flavonoids aglycon extract, also a preparation method of radix scutellariae total flavonoids aglycon extract and functions of treating and resisting renal fibrosis. Content of total flavonoids aglycons in the radix scutellariae total flavonoids aglycon extract is 45-70%. The extract is mainly composed of scutellarein, wogonin, oroxylin A and other aglycons. The preparation method of the extract is as follows: crushing radix scutellariae roots, screening by a No. 1 screen; adding 1-5 times amount of water, uniformly stirring, keeping temperature for enzymolysis at 32-40 DEG C for 5-9 hours, baking under vacuum at 60 DEG C, adding 2-8 times amount of ethyl acetate, continuously extracting for 6-10 hours, depressurizing, recycling solvent, thus obtaining total flavonoids aglycon extract. The extract can obviously relieve renal fibrosis symptoms when irrigated into a mouse stomach with renal fibrosis caused by mercuric chloride moulding and when a group of mice having the extract is compared with group of mice without having the extract. Results suggest that the total flavonoids aglycon extract has a dose dependence in preventing renal fibrosis.

Description

technical field [0001] The invention relates to a total flavonoid aglycone extract of Scutellaria baicalensis, its preparation method and application, specifically the preparation of the main aglycone contained in Scutellaria baicalensis and its application in anti-renal fibrosis, belonging to the technical field of medicine. Background technique [0002] Renal tissue cells are rich in metabolism, and tissue hypoxia and oxidative stress damage are prone to occur after damage. Oxidative stress can not only lead to lipid peroxidation and inflammatory damage in tissues, but also stimulate the expression of pro-fibrotic factors in renal tubular epithelial cell transdifferentiation, thereby leading to renal fibrosis; and further aggravate inflammatory damage by activating the NF-κB pathway with fibrosis. Renal fibrosis is a common pathway for the development of various chronic kidney diseases to chronic renal failure and other end-stage renal diseases, including renal interstiti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/539A61P13/12
Inventor 张永煜刘成海魏世道谢红东杨珂陶艳艳
Owner SHANGHAI UNIV OF T C M
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