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Clostridium butyricum, fermented product of clostridium butyricum, microbial inoculum containing clostridium butyricum and animal feed additive

A technology of Clostridium butyricum and fermented product, which is applied in the field of microorganisms, can solve the problems of complex nutritional composition, growth and reproduction of common Clostridium butyricum, limitation of acid production, complex environment, etc., and achieves reduction of diarrhea rate and good growth adaptability. And the effect of fermentation acid production capacity and improving feed to weight ratio

Active Publication Date: 2022-08-09
江苏三仪生物工程有限公司 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the intestinal tract of animals, the environment is complex and the nutritional composition is complex. The growth, reproduction and acid production of Clostridium butyricum in the intestinal tract of animals are limited to a certain extent.

Method used

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  • Clostridium butyricum, fermented product of clostridium butyricum, microbial inoculum containing clostridium butyricum and animal feed additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1 - Isolated strains

[0068] A 60-day-old healthy piglet was selected and slaughtered in Fengyuan Pig Farm, Pizhou, Jiangsu Province, and the contents of the cecum and intestinal tract were taken, and the intestinal contents were water-bathed at 80 °C for 5 minutes. Put it in the ice box and take it back to the laboratory. In the ultra-clean bench, pick a ring of the above intestinal contents after water bath at 80°C, inoculate it in RCM liquid medium, and culture it at 37°C for 24 hours in an anaerobic environment to enrich and culture Clostridium butyricum. Each of the above enriched cultures was picked, streaked on 20 plates on the RCM medium, put into a sealed box, added to an anaerobic bag, and cultured at 37°C for 24 hours.

[0069] On the RCM plate, pick out the white or milky white or gray-white, round, irregular edge, moist, no bulge in the middle, and the colony edge is slightly thinner than the middle, mark and carry out Gram staining (Gram Positiv...

Embodiment 2

[0073] Example 2 - Ultraviolet mutagenesis and screening of strains

[0074] The purified 1# colony is processed according to the following steps:

[0075] (1) Pick 1# colony, inoculate it into a 100 mL shake flask containing 100 mL of RCM liquid medium, culture it anaerobically at 37° C. for 18 hours, and observe microscopically that the cells are clostridial and do not form spores. Take 1mL of this culture and dilute it to 10 in 9mL of sterile water. -1 Diluted bacterial suspension, repeat the above operation, dilute to 10 -5 concentration of bacterial suspension, and spread the above bacterial suspension on RCM plate.

[0076] (2) Turn on the UV mutagenesis box and preheat for 20 minutes until the light wave is stable. The above RCM plate coated with bacterial solution was placed in a UV mutagenesis box. Lift the lid of the petri dish, irradiate with light wavelength 360nm, power 25W ultraviolet lamp irradiation distance is 30cm, and the irradiation dose is 10s, 15s, 20...

Embodiment 3

[0078] Example 3 - Growth of each strain in jejunal intestinal juice

[0079] 200 mL of jejunal intestinal juice was collected in the Pizhou Zhengda slaughterhouse, frozen, and brought back to the laboratory. After thawing treatment, it was centrifuged at low speed to remove impurities. The above liquid was sterilized by filtration with a 0.5 μm filter, and then divided into 10 mL centrifuge tubes, and the volume of each tube was 5 mL. Pick out one ring each of the germinated strains, L11, L15, L22, L34, L42, DS20 colonies, respectively, inoculate them in the above-mentioned jejunal intestinal juice, put them into a closed anaerobic box, add an anaerobic bag, and place for 24 hours. It was found that the colonies of the starting strain in the jejunal fluid were very small, or could hardly grow. Other strains can grow normally, and the pH values ​​were measured respectively. The results showed that the pH values ​​in the L11, L15, L22, L34, L42, and DS20 tubes were 7.0, 6.8, 6...

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Abstract

The clostridium butyricum is named as a Clostridium butyricum DS20 strain, the preservation unit is China Center for Type Culture Collection, the preservation number is CCTCC NO: M 2022581, the preservation date is May 10, 2022, and the preservation address is Wuhan University, Wuhan, China, on May 10, 2022. The clostridium butyricum is high in acid yield, good in growth adaptive capacity and fermentation acid production capacity in intestinal juice and excellent in acid resistance, bile salt resistance and heat resistance, and when the clostridium butyricum is used as an animal feed additive, the intestinal health of animals can be remarkably improved, and the feed utilization rate can be increased.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to Clostridium butyricum, its fermented product, a bacterial agent containing the same, and an animal feed additive. Background technique [0002] Since the antibacterial drugs for livestock and poultry were put into use, the livestock and poultry breeding industry has developed rapidly. The survival rate of breeding has been continuously improved, the breeding cycle has been gradually shortened, and the breeding income has been increasing. However, the use of additives such as antibiotics brings us convenience, but also creates many new problems. In recent years, the aquaculture industry has received extensive attention from all walks of life due to the misuse and abuse of antibiotics leading to bacterial resistance. To achieve sustainable development of animal husbandry, the restriction and prohibition of antibiotics in feed has become an inevitable trend. Therefore, the r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/01C12N13/00A23K10/18C12R1/145C12R1/25C12R1/125C12R1/07C12R1/01
CPCC12N1/20C12N15/01C12N13/00A23K10/18A23V2400/169Y02E50/10
Inventor 江国托刘秋晨刘星刘艳单春乔李娟王岩于洪敏翟宏旭
Owner 江苏三仪生物工程有限公司
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