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Lanqin oral solution fingerprint spectrum establishment method, fingerprint spectrum of Lanqin oral solution and application of fingerprint spectrum

A technology of Lanqin oral liquid and fingerprints, which is applied in the field of drug analysis, can solve the problems of not being able to reflect the overall curative effect of traditional Chinese medicine as a whole, and achieve the effect of ensuring safety and effectiveness

Active Publication Date: 2019-08-09
YANGTZE RIVER PHARMA GRP JIA NGSU LONGFENGTANG TRADITIONAL CHINESE MEDICINE CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The quality of drugs is the premise to ensure the safety and effectiveness of clinical medication. Currently, the method commonly used to evaluate the quality of Lanqin oral liquid preparations is to control the content of geniposide, but this method cannot reflect the overall efficacy of traditional Chinese medicine as a whole.

Method used

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  • Lanqin oral solution fingerprint spectrum establishment method, fingerprint spectrum of Lanqin oral solution and application of fingerprint spectrum
  • Lanqin oral solution fingerprint spectrum establishment method, fingerprint spectrum of Lanqin oral solution and application of fingerprint spectrum
  • Lanqin oral solution fingerprint spectrum establishment method, fingerprint spectrum of Lanqin oral solution and application of fingerprint spectrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] Example 1 The establishment method and methodological investigation of the fingerprint of Lanqin Oral Liquid

[0089] 1. Method for establishing the fingerprint of Lanqin Oral Liquid

[0090] The method for establishing the fingerprint of Lanqin Oral Liquid comprises the following steps:

[0091] (1) Preparation of the test solution: take 19 different batches of Lanqin oral liquid in the above table 1, accurately draw 200 μl into a 10mL centrifuge tube, add 4.8mL of methanol to dilute, mix well and pass through a 0.45 μm micro Pore ​​filter membrane, as need testing solution;

[0092] (2) Preparation of reference substance solution: Take an appropriate amount of baicalin reference substance, weigh it accurately, add 70% methanol to dissolve, and prepare the content to be 208 μg·mL -1 The reference substance solution, respectively take adenosine reference substance, Yichun reference substance, cortex base hydrochloride reference substance, chlorogenic acid reference su...

Embodiment 2

[0138] Example 2 Detection and Methodological Investigation of the Multi-wavelength Quantitative Components of the Lanqin Oral Liquid Fingerprint

[0139] 1. Detection of multi-wavelength quantitative components

[0140] Adenosine, Yichun, phellodine hydrochloride, chlorogenic acid, magnolialine, geniposide, benzoic acid, berberine hydrochloride, wogonin, baicalein, wogonin 12 kinds of drug efficacy index ingredients, retained The times are: 2.58, 12.56, 15.18, 16.68, 17.23, 21.53, 40.13, 41.45, 47.43, 55.69, 60.88, 64.01min. Turn on the DAD detector for full-wavelength scanning, compare the peak areas of each drug efficacy index component under different wavelength conditions, and obtain the optimal detection wavelength of each index component. Acid, magnolialine, geniposide, benzoic acid and berberine hydrochloride have greater absorption at 230nm; baicalin, wogonoside, baicalein, wogonin have greater absorption at 276nm. Finally, select and record the retention time and p...

Embodiment 3

[0215] Example 3 Establishment of Fingerprints of Lanqin Oral Liquid Key Process Intermediates

[0216] 1. Fingerprint of Concentration Process Intermediate I

[0217] Take an appropriate amount of 9 different batches of Lanqin concentrated process intermediate I in the above table 2, add 60% methanol aqueous solution to dilute, mix well, and pass through a 0.45 μm microporous membrane as the test solution. Sample injection by chromatographic conditions in Example 1, obtain the liquid chromatography of need testing solution. Import the liquid chromatogram into the "Chromatographic Fingerprint Similarity Evaluation System 2004A Edition" compiled by the National Pharmacopoeia Committee to process the chromatogram data. Obtain the overlay chromatogram of concentrated process intermediate I (see Figure 20 ) and the control map (see Figure 21 ), the fingerprints of 9 batches of Lanqin concentrated process intermediate I were analyzed for similarity with the generated control f...

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Abstract

The invention discloses a Lanqin oral solution fingerprint spectrum establishment method, a fingerprint spectrum of a Lanqin oral solution and application of the fingerprint spectrum. A test solutionis prepared from the Lanqin oral solution, a reference substance solution is a solution which dissolves adenosine, epigoitrin, phellodendrine chloride, chlorogenic acid, magnoflorine, geniposide, benzoic acid, berberine hydrochloride, baicalin, wogonoside, baicalein and wogonin, and the liquid chromatography is measured by using a high-performance liquid chromatograph; the obtained liquid chromatography is introduced into a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system for analysis, and the Lanqin oral solution fingerprint spectrum is obtained after multi-point correction, data matching and analysis. According to the Lanqin oral solution fingerprint spectrum establishment method and the fingerprint spectrum of the Lanqin oral solution, the quality information of the Lanqin oral solution can be comprehensively reflected, and uniform and stable product quality is ensured.

Description

technical field [0001] The invention relates to the field of drug analysis, in particular to a method for establishing fingerprints of Lanqin oral liquid, fingerprints and applications thereof. Background technique [0002] Lanqin Oral Liquid is a pure Chinese medicine preparation independently developed by Yangzijiang Pharmaceutical Group based on clinically proven prescriptions. Heat, analgesia, anti-inflammatory, antibacterial, antiviral five-in-one effect, mainly used for acute pharyngitis, sore throat, dry throat, burning throat caused by lung and stomach excess heat syndrome, upper respiratory tract infection, etc. It has obvious antiviral, antibacterial and anti-inflammatory effects, especially in reducing fever, eliminating sore throat, cough and other symptoms, with definite curative effect. Because of its wide curative effect, few adverse reactions, and a wide range of users, Lanqin Oral Liquid has attracted more and more attention from the society, and its develo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8809
Inventor 陈钟任晓亮姚仲青曹雪晓游慧邱峰
Owner YANGTZE RIVER PHARMA GRP JIA NGSU LONGFENGTANG TRADITIONAL CHINESE MEDICINE CO LTD
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