Technique of preparing high-purity wogonin by extraction and separation method

A high-purity technology for wogonin, which is applied in the field of extraction, separation and preparation of wogonin, the active ingredient in Scutellaria baicalensis, can solve the problems of low wogonin content and difficulty in meeting market requirements

Inactive Publication Date: 2008-12-31
孙蓉
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Problems solved by technology

It solves the problem that the content of wogonin in commercially ava

Method used

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Examples

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Effect test

Embodiment 1

[0007] Example 1: take Scutellaria baicalensis medicinal material, crush it through a 10-mesh sieve, add water to soak for 4 hours, filter, add 15 times the amount of ethanol to the medicinal residue, extract twice at 70°C, for 2 hours each time, filter, combine the filtrates, and depressurize Concentrate to a concentrated solution with a relative density of 1.05 to 1.15 (measured at 40°C), put the concentrated solution into a macroporous adsorption resin separation column, use a wet method to pack the column, wash the resin column with distilled water until the washing liquid is colorless, discard the washing liquid Next, use 95% ethanol to elute, collect the eluate of the second yellow band, and concentrate under reduced pressure to a concentrated solution with a relative density of 1.05-1.15 (measured at 40°C). Then high-speed countercurrent chromatography was used to separate the concentrated solution. At room temperature, the system 1 (n-hexane-acetone-ethanol-water=6:4:4:...

Embodiment 2

[0008] Example 2: Take Scutellaria baicalensis, crush through a 10-mesh sieve, add 1% hydrochloric acid aqueous solution to soak for 6 hours, filter, add 12 times the amount of 85% ethanol to extract three times at 60 ° C for 1.5 hours each time, filter, and combine The filtrate is concentrated under reduced pressure into a concentrated solution with a relative density of 1.05 to 1.15 (measured at 40°C). The concentrated solution is placed in a macroporous adsorption resin separation column, and the column is packed by a wet method. The resin column is washed with distilled water until the washing solution is colorless. , discarded the water wash, and eluted with 95% ethanol, collected the eluate of the second yellow band, and concentrated under reduced pressure to a concentrated solution with a relative density of 1.05-1.15 (measured at 40°C). Then use high-speed countercurrent chromatography to separate the concentrated solution. At room temperature, the system cyclohexane-ch...

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Abstract

The invention relates to a preparation technology for extracting and separating high-purity wogonin; the scutellaria material is crushed and soaked with water; then the material is joined with a proper amount of solvent and then extracted, filtered and concentrated; the concentrated liquid is separated through macroporous resin column chromatography and high-speed countercurrent chromatography successively to prepare the high-concentration wogonin. The invention has stable process and high wogonin content in the finished product; the content of wogonin reaches more than 96%, based on the measurement through high-performance liquid phase method.

Description

Technical field: [0001] The invention belongs to a method for extracting and separating components or parts of traditional Chinese medicines. Specifically, the invention relates to a method for extracting, separating and preparing wogonin, an active ingredient in Scutellaria baicalensis, a traditional Chinese medicine. Background technique: [0002] Scutellaria baicalensis is a commonly used traditional Chinese medicine, which has the functions of clearing heat and purging fire, detoxifying, hemostasis, and preventing miscarriage. Its active ingredients are flavonoids. At present, there are more than a dozen kinds of flavonoids extracted and identified from Scutellaria baicalensis, mainly including baicalin, wogonoside, baicalein, wogonin, and scutellarin. According to literature reports, wogonin has anticancer properties. , antibacterial, diuretic, antispasmodic, heat-clearing, analgesic and other effects. However, due to the backwardness of the existing technology and the...

Claims

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Application Information

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IPC IPC(8): C07D311/30A61K36/539
Inventor 孙蓉于晓张作平任海勇衣银萍韩艳
Owner 孙蓉
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