Method for simultaneously extracting baicalin, baicalein and wogonin from scutellaria baicalensis

A technology for wogonin and baicalein, which is applied in the field of traditional Chinese medicine extraction, can solve the problems of resource waste, component loss, loss and the like, and achieves the effects of less waste liquid, reduced loss, and efficient extraction.

Active Publication Date: 2015-05-13
SHANDONG ACAD OF CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] At present, there are many studies on the extraction process of the effective components of Scutellaria baicalensis, but most of them are only for one component in Scutellaria baicalensis, such as the extraction of baicalin by acid precipitation and alkali dissolution, and the extraction of baicalein by enzymatic hydrolysis. , the other components are lost
At the same time, because the extraction methods of each active ingredient are different, it is impossible to simply combine several methods for extracting different active ingredients of Scutellaria baicalensis to achieve the purpose of extracting multiple active ingredients from Scutellaria baicalensis at the same time
[0004] In addition, in the purification method of Scutellaria baicalensis extract included in the Pharmacopoeia, the method of water extraction and acid precipitation is used for purification, the purpose is to increase the content of baicalin. After purification, the content of baicalin in the final extract obtained is not less than 85%, but the purified In the process, baicalein, wogonin, wogonin and other components are all lost, resulting in a waste of resources

Method used

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  • Method for simultaneously extracting baicalin, baicalein and wogonin from scutellaria baicalensis
  • Method for simultaneously extracting baicalin, baicalein and wogonin from scutellaria baicalensis
  • Method for simultaneously extracting baicalin, baicalein and wogonin from scutellaria baicalensis

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Experimental program
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Embodiment 1

[0038] Step 1. Simultaneous extraction: take Scutellaria baicalensis pieces, add 12 times the mass of water to heat and reflux for extraction for 2 hours, filter the extract, add 10 times the amount of water to the filter residue and extract twice, each time for 1.5 hours, and combine the filtrates to obtain baicalin and baicalein and the mixed crude extract of wogonin.

[0039] Step 2. Separation: Concentrate the crude extract from the previous step under reduced pressure to 0.2 g crude drug per 1 mL, load the sample on a D101 macroporous adsorption resin column at a flow rate of 4 BV / h, the sample volume is 1 BV, and elute with water first Until the effluent is colorless, it is eluted with 10BV 60% ethanol and 6BV 80% ethanol in turn, and the 60% ethanol eluate is collected. The main component is baicalin (see figure 2 ), collect 80% ethanol eluate, the main components are baicalein and wogonin (see image 3 ). Concentrate the baicalin eluate to contain 0.5 g crude drug p...

Embodiment 2

[0041] Step 1. Simultaneous extraction: Take Scutellaria baicalensis slices, add 15 times the amount of mass to heat and reflux for extraction for 1.8 h, filter the extract, add 15 times the amount of water to the filter residue and extract once more, combine the filtrates to obtain baicalin, baicalein and wogonin Combine the crude extracts.

[0042]Step 2. Separation: Concentrate the crude extract from the previous step to 0.5 g crude drug per 1 mL, load the sample on the AB-8 macroporous adsorption resin column at a flow rate of 4 BV / h, and the sample volume is 0.5 BV. Eluted with water until the effluent was colorless, then eluted with 8BV 70% ethanol and 4BV 80% ethanol respectively, collected 70% ethanol eluate, the main component was baicalin, collected 80% ethanol eluate, the main component was Baicalein and Wogonin. Concentrate the baicalin eluate to contain 1 g crude drug per mL, adjust the pH value to 2.1 with 1 mol / L sulfuric acid, incubate at 70 °C for 1 h, let st...

Embodiment 3

[0044] Step 1. Simultaneous extraction: take Scutellaria baicalensis slices, add 20 times the mass of water, heat and reflux for extraction for 1.5 h, filter the extract, add 15 times the amount of water to the filter residue and extract once more, combine the filtrates, and obtain baicalin, baicalein and wogonin Combine the crude extracts.

[0045] Step 2. Separation: Concentrate the crude extract from the previous step under reduced pressure to 0.3 g crude drug per 1 mL, and load the HPD-100 macroporous adsorption resin column at a flow rate of 4 BV / h. The sample volume is 1 BV. Eluted with water until the effluent was colorless, then eluted with 9BV 60% ethanol and 6BV 75% ethanol respectively, collected 60% ethanol eluate, the main component was baicalin, collected 75% ethanol eluate, the main component was Baicalein and Wogonin. The crude extract of baicalin was concentrated under reduced pressure to contain 1.5 g crude drug per mL, the pH value was adjusted to 1.8 with ...

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Abstract

The invention discloses a method for simultaneously extracting baicalin, baicalein and wogonin from scutellaria baicalensis. The method comprises the following steps: extracting scutellaria baicalensis with water and concentrating to obtain a concentrated solution; rinsing the concentrated solution on a macroporous adsorption resin column till the solution is colorless, and then respectively eluting by using 60-70vol% ethyl alcohol and 75-80vol% ethyl alcohol and collecting eluants respectively; concentrating the eluant containing baicalin under reduced pressure, regulating pH, preserving the heat, standing, filtering to obtain pure baicalin; and evaporating the eluant containing baicalein and wogonin to dryness, eluting with methyl alcohol on a gel column, collecting baicalein fraction and wogonin fraction, and removing methyl alcohol out of the fractions to obtain pure baicalein and pure wogonin. The method has the advantages that the extraction rate is high, the loss of active ingredients is reduced and the resource utilization rate of scutellaria baicalensis is improved.

Description

technical field [0001] The invention relates to a method for extracting various active ingredients in traditional Chinese medicine, in particular to a method for simultaneously extracting baicalin, baicalein and wogonin from scutellaria baicalensis, belonging to the technical field of traditional Chinese medicine extraction. Background technique [0002] Scutellaria baicalensis (Latin name: Scutellaria baicalensis Georgi), also known as camellia root and earth gold tea root, is a perennial herb of the Lamiaceae Scutellaria genus. It has the functions of clearing away heat and dampness, purging fire and detoxification, stopping bleeding, and preventing miscarriage. Scutellaria baicalensis contains baicalin, baicalein, wogonin, wogonoside, neobaicalein, and other flavonoids. [0003] At present, there are many studies on the extraction process of the effective components of Scutellaria baicalensis, but most of them are only for one component in Scutellaria baicalensis, such ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08C07D311/30
Inventor 于蓓蓓于宗渊闫雪生
Owner SHANDONG ACAD OF CHINESE MEDICINE
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