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Human parainfluenza virus I, II, III, and IV-type quadruple-PCR detection kit, and detection method thereof

A human parainfluenza virus, detection kit technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial determination/inspection, etc. Simple and effective for improving amplification efficiency

Active Publication Date: 2016-03-30
深圳生科原生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a four-fold PCR detection kit for human parainfluenza virus types I, II, III, and IV, which aims to solve the problems of cumbersome operation and low accuracy in the prior art

Method used

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  • Human parainfluenza virus I, II, III, and IV-type quadruple-PCR detection kit, and detection method thereof
  • Human parainfluenza virus I, II, III, and IV-type quadruple-PCR detection kit, and detection method thereof
  • Human parainfluenza virus I, II, III, and IV-type quadruple-PCR detection kit, and detection method thereof

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Embodiment 1

[0096] A quadruple PCR detection kit for human parainfluenza virus types I, II, III, and IV, including a PCR reaction solution, a negative control and a positive control, wherein the specification of the PCR reaction solution is: 8 tubes×6 strips, 20 μl / tube, and the components are shown in Table 1 below; the positive control specification is 1 tube, 40 μl / tube, and the components are RNA transcribed in vitro after the synthetic sequence of the detection region of human parainfluenza virus type I, II, III, and IV, using Tris-EDTA The buffer solution (0.01MpH8.0) was diluted and stored frozen; the specification of the negative control was 1 tube, 40 μl / tube, and the composition was Tris-EDTA buffer solution ( 0.01M pH8.0).

[0097] Table 1

[0098]

[0099]

[0100] A quadruple PCR detection method for human parainfluenza virus types I, II, III, and IV. The method uses the above-mentioned quadruple PCR detection kit for human parainfluenza virus types I, II, III, and IV...

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Abstract

The invention belongs to the technical field of virus detection kit, and provides a human parainfluenza virus I, II, III, and IV-type quadruple-PCR detection kit, and a detection method thereof. The human parainfluenza virus I, II, III, and IV-type quadruple-PCR detection kit comprises human parainfluenza virus specific primers and gene probes; and in the detection method, the human parainfluenza virus I, II, III, and IV-type quadruple-PCR detection kit is used for detection. Compared with the prior art, the human parainfluenza virus I, II, III, and IV-type quadruple-PCR detection kit and the detection method possess following advantages: detection of human parainfluenza virus I, II, III, and IV types can be realized at the same time; sensitivity, specificity, and accuracy are high; and detection period is short.

Description

technical field [0001] The invention belongs to the technical field of virus detection kits, in particular to a quadruple PCR detection kit for human parainfluenza virus types I, II, III and IV and a detection method thereof. Background technique [0002] Real-time fluorescence quantitative PCR is widely used in basic scientific research, clinical diagnosis, disease research and drug development and other fields. The basic principle is: a fluorescent chemical substance is introduced into the PCR reaction system. The product continues to accumulate, and the intensity of the fluorescent signal increases proportionally. After each cycle, a fluorescence signal intensity is collected, so that the change of the product amount can be monitored through the change of the fluorescence intensity, and a fluorescence amplification curve is obtained, such as figure 1 As shown, in the exponential amplification stage of the curve, there is a linear relationship between the logarithmic valu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12N15/11
CPCC12Q1/686C12Q1/701C12Q2600/16C12Q2561/113C12Q2563/107C12Q2531/113
Inventor 王茵茵田仁鹏吴成贡邓春兴
Owner 深圳生科原生物有限公司
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