Real-time fluorescent quantitative PCR probe primer group and kit for rapid qualitative typing detection of four types of human parainfluenza viruses

A real-time fluorescence quantification, primer set technology, applied in microorganism-based methods, microorganism determination/inspection, microorganisms, etc., to save time and reagent costs, and to achieve the effect of simple interpretation

Inactive Publication Date: 2021-08-31
北京华诺奥美基因生物科技有限公司
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Problems solved by technology

There is no real-time fluorescent quantitative PCR kit for rapid qualitative typing and detection of four types of human parainfluenza viruses in the prior art

Method used

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  • Real-time fluorescent quantitative PCR probe primer group and kit for rapid qualitative typing detection of four types of human parainfluenza viruses
  • Real-time fluorescent quantitative PCR probe primer group and kit for rapid qualitative typing detection of four types of human parainfluenza viruses
  • Real-time fluorescent quantitative PCR probe primer group and kit for rapid qualitative typing detection of four types of human parainfluenza viruses

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Embodiment 1

[0070] A real-time fluorescent quantitative PCR kit for rapid qualitative typing and detection of four types of human parainfluenza viruses (comprising HPIV-1, HPIV-2, HPIV-3 and HPIV-4a / 4b), the PCR kit contains reverse Recording enzyme, high-efficiency DNA polymerase, dNTPs, probe primer set specially designed for four types of parainfluenza virus, qPCR buffer suitable for quantitative analysis, ROX reference fluorescent dye, blank control and positive control. The primer sequences in the probe primer set are shown in Table 1, and the probe sequences in the probe primer set are shown in Table 2.

[0071] Table 1 Primer Sequence

[0072]

[0073]

[0074] Table 2 Probe sequence

[0075]

[0076] The method of detection using the above kit is as follows:

[0077] (1) As shown in Table 3, a fluorescent quantitative PCR reaction system was prepared.

[0078] Table 3 Fluorescent quantitative PCR reaction system

[0079]

[0080]

[0081] (2) Fluorescent quantit...

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Abstract

The invention discloses a real-time fluorescent quantitative PCR probe primer group and a kit for rapid and qualitative typing detection of four types of human parainfluenza viruses. The kit comprises the probe primer group specially designed for the four types of human parainfluenza viruses, i.e., HPIV-1, HPIV-2, HPIV-3 and HPIV-4; primer sequences in the probe primer group are as shown in SEQ ID NO.1-27; and probe sequences in the probe primer group are as shown in SEQ ID NO. 28 to 38. According to the invention, a reaction system of the real-time fluorescent quantitative PCR kit for rapid qualitative typing detection of the four types of human parainfluenza viruses aims at conserved regions of genomes of the four types of human parainfluenza viruses and can be used for rapid detection of parainfluenza viruses in nasopharynx swabs or sputum samples, and detection results can be used for auxiliary diagnosis of symptoms such as cough, fever, pneumonia and the like causedby parainfluenza and rapidly distinguishing parainfluenza patients from other fever and pneumonia patients.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a real-time fluorescent quantitative PCR probe primer set and kit for rapid qualitative typing and detection of four types of human parainfluenza viruses. Background technique [0002] Human parainfluenza virus (HPIV) is a group of viruses that can cause upper and lower respiratory tract infections, and is divided into four categories: HPIV-1, HPIV-2, HPIV-3 and HPIV-4 (type 4 There are two subtypes of parainfluenza, 4a and 4b). HPIVs belong to the Paramyxoviridae family and are enveloped RNA viruses. HPIVs commonly cause upper and lower respiratory illness in infants, young children, older adults, and people with weakened immune systems, but anyone can become infected. After being infected, it takes about 2 to 7 days for symptoms to appear. Both HPIV-1 and HPIV-2 can cause coughing, with HPIV-1 being one of the most common viruses that cause colds in infants and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6851C12N15/11C12R1/93
CPCC12Q1/701C12Q1/6851
Inventor 张可欣王慧魏星
Owner 北京华诺奥美基因生物科技有限公司
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