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Serum-12 type haemophilus lus paradis vaccine strain and application thereof

A technology of Haemophilus suis and vaccine strains, applied in bacteria, antibacterial drugs, medical preparations containing active ingredients, etc. long term, reduced morbidity and mortality, and reduced economic losses

Inactive Publication Date: 2015-03-25
扬州优邦生物药品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the bacterial diseases represented by Haemophilus parasuis are increasingly harmful to the pig industry, which leads to the use of a large number of antibiotics for treatment, resulting in the continuous enhancement of bacterial drug resistance and the emergence of drug-resistant strains, especially in parasuis Haemophilus is very prone to drug resistance, resulting in poor treatment effect, so farmers increase the amount of antibiotics, causing a vicious circle

Method used

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  • Serum-12 type haemophilus lus paradis vaccine strain and application thereof
  • Serum-12 type haemophilus lus paradis vaccine strain and application thereof
  • Serum-12 type haemophilus lus paradis vaccine strain and application thereof

Examples

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Embodiment 1

[0019] Example 1: Isolation, identification and biological characteristics of Haemophilus parasuis serotype 12

[0020] 1. Isolation of Haemophilus parasuis

[0021] 1. Preparation of culture medium

[0022] (1) The preparation of TSA, add TSA 40g to 1000mL distilled water, fully dissolve, after 121 ℃ 30min autoclaving, put it at room temperature, when the temperature drops to about 50 ℃, add inactivated calf serum to TSA respectively and NAD (nicotinamide dipurine), so that the final mass concentration percentages of serum and NAD reach 5% and 0.01%, respectively, and then pour it into a disposable plate. After cooling and solidifying, the package is placed at 4°C for use. It is best not to store the TSA plate at 4°C for more than 30 days, so as to prevent the medium in the plate from drying out and affecting the effect.

[0023] (2) The preparation of TSB, add 30g of TSB to 1000mL of distilled water, after fully dissolving, after autoclaving at 121°C for 30min, place it at...

Embodiment 2

[0073] Embodiment 2: the application of isolated strain SHCM10 strain in Haemophilus parasuis inactivated vaccine

[0074] 1. Strain selection

[0075] Select serotype 12 Haemophilus parasuis isolate SHCM10, which has been preserved in China Center for Type Culture Collection, address: Wuhan, China, Wuhan University, preservation number: CCTCC NO: M 2014261, preservation date: June 15, 2014;

[0076] 2. Preparation of production seeds

[0077] Take the freeze-dried SHCM10 strain, add sterile saline to dissolve, inoculate on TSA medium by streaking, culture at 37°C for 24 hours, pick three typical colonies, inoculate them into 3.0mL TSB liquid medium, and culture with shaking at 37°C 20-22 hours, harvest the culture, conduct a purity test (according to the appendix of the current "People's Republic of China Veterinary Pharmacopoeia", the following purity test is also the same), after passing the test, inoculate it into TSB liquid medium at a ratio of 1% (that is, add TSB 1% o...

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Abstract

The invention relates to a serum-12 type haemophilus lus paradis vaccine strain. The classified name of the vaccine strain is haemophilus lus paradis, the strain name is SHCM10 and the vaccine strain is preserved in the China Center for Type Culture Collection on June 15, 2014 with the preservation number of CCTCC NO:M 2014261. The invention further relates to an application of the serum-12 type haemophilus lus paradis vaccine strain in preparation of a haemophilus lus paradis inactivated vaccine. The serum-12 type haemophilus lus paradis SHCM10 strain is stable in biology, has a strong pathogenicity to a piglet, and has a good immunogenicity when being inactivated and vaccinated on the piglet. A univalent vaccine prepared from the vaccine strain serving as a vaccine candidate strain has good safety, can produce a relatively high antibody on the piglet, has long duration and good immune potency, and can be used for resisting attack of homotype wild strains. After a pig group is immunized, the morbidity and death rate are remarkably reduced, the economic loss of a piggery is reduced, and the immunizing effect of the vaccine strain is the same as or superior to that of existing commercial vaccines on the market.

Description

technical field [0001] The invention relates to the field of Haemophilus parasuis inactivated vaccines in veterinary biological products, in particular to a serotype 12 Haemophilus parasuis vaccine strain, and also relates to the application of the vaccine strain in the preparation of inactivated vaccines and the preparation of inactivated vaccines Vaccine method. Background technique [0002] Haemophilus parasuis (Haemophilus parasuis, HPS) is a G-coccus bacillus, an opportunistic pathogenic bacteria in pigs, which can cause Haemophilus parasuis disease. The disease was first reported by German scholar Glasser in 1910, and now it is widely distributed all over the world. At present, Haemophilus parasuis infection is one of the main causes of high mortality of piglets in pig farms. The disease is currently posing a great threat to Chinese pig herds. With the prevalence of porcine reproductive and respiratory syndrome (PRRS, blue ear disease), Haemophilus parasuis has cause...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K39/102A61P31/04C12R1/21
CPCA61K39/102C12N1/205C12R2001/21
Inventor 钱钟李甜甜王郑徐萍商俊
Owner 扬州优邦生物药品有限公司
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