Porcine streptococcus disease and haemophilus parasuis disease combined inactivate vaccine and preparation method thereof

A technology of Haemophilus suis and Streptococcus suis, which is applied in the field of preparation of Streptococcus suis, Haemophilus parasuis disease dual inactivated vaccine, and Haemophilus parasuis disease dual inactivated vaccine, can solve waste, antibiotics Problems such as drug residues and ineffective drug treatment

Active Publication Date: 2012-01-25
WUHAN KEQIAN BIOLOGY CO LTD
View PDF6 Cites 22 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the only vaccines used on the market are inactivated vaccines for the prevention of Streptococcus suis and Haemophilus parasuis alone
The inactivated vaccine for simultaneously preventing Streptococcus suis and Haemophilus parasuis has not been r...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Porcine streptococcus disease and haemophilus parasuis disease combined inactivate vaccine and preparation method thereof
  • Porcine streptococcus disease and haemophilus parasuis disease combined inactivate vaccine and preparation method thereof
  • Porcine streptococcus disease and haemophilus parasuis disease combined inactivate vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] A kind of preparation scheme of streptococcosis suis (type 2), Haemophilus parasuis disease (type 4, type 5) double inactivated vaccine, its steps are as follows:

[0067] This product is inoculated in a suitable medium by Streptococcus suis type 2 LT strain, Haemophilus parasuis type 4 MD0322 strain and Haemophilus parasuis type 5 SH0165 strain, and the culture is easily inactivated by formaldehyde to remove residues. Toxin, formulated with white oil adjuvant, is used to prevent various bacterial infections caused by Streptococcus suis type 2 and Haemophilus parasuis types 4 and 5. This includes several of the most important bacterial diseases currently prevalent. The strains used in the manufacture and inspection of this product are Streptococcus suis 2-LT strain (CCTCC NO: M2011282), Haemophilus parasuis 4-MD0322 strain (CCTCC NO: M2011283) and Haemophilus parasuis 5-SH0165 strain ( CCTCC NO: M2011284) are all preserved in the China Center for Type Culture Collectio...

Embodiment 5

[0085] Example 5 Efficacy Research

[0086] 1 Test plan:

[0087] With 30 healthy weaned piglets at the age of 28 to 35 days, 15 of them were intramuscularly injected with 2ml of the vaccine prepared in Example 1, containing 1 dosage, and the second immunization was carried out 21 days after the first immunization; the remaining 15 were used as untreated piglets. Immunization control group. After immunization, the body temperature of the animals was measured, and the clinical manifestations were observed.

[0088] Fourteen days after the second immunization, Streptococcus suis type 2 was challenged intravenously, and Haemophilus parasuis was challenged intraperitoneally. Use 2.0×10 respectively 6 CFU Streptococcus suis 2-LT strain, 7.0×10 9 CFU of Haemophilus parasuis 4-MD03220 strain and 6.0×10 9 The Haemophilus parasuis 5-SH0165 strain of CFU was challenged. After the challenge, the clinical symptoms and death were observed, and the protection rate of each batch of vacc...

Embodiment 2

[0099] Example 2: Isolation and identification of Streptococcus suis 2-LT strain (Streptococcus suis 2-LT).

[0100] The disease material to be separated—the disease material of each tissue was obtained by the applicant's inventor in September 2004 from a pig farm in Luotian, Hubei Province, which had neurological symptoms and acutely died pigs. The specific separation method is as follows: aseptically collect heart blood and inoculate it on a TSA plate, and observe after culturing at 37°C for 12-24 hours. Pick small colonies with a diameter of 0.1mm-1.0mm, off-white, translucent, smooth, round, and with neat edges, and inoculate them in TSB liquid medium for overnight culture on a shaker at 37°C. Purely cultured bacteria were subjected to Gram staining for microscopic examination. Then observed with an optical microscope, Gram staining was positive, doubled or short streptococci, which were subcultured and purified.

[0101] The classification and identification of Streptoc...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a porcine streptococcus disease and haemophilus parasuis disease combined inactivate vaccine and a preparation method thereof. The preparation method comprises the following steps of: a, respectively carrying out enrichment culture on a porcine streptococcus strain, a haemophilus parasuis strain and a haemophilus parasuis strain to obtain a porcine streptococcus strain bacterial solution, a haemophilus parasuis strain bacterial solution and a haemophilus parasuis strain bacterial solution; b, respectively adding a formaldehyde solution into the porcine streptococcus strain bacterial solution, the haemophilus parasuis strain bacterial solution and the haemophilus parasuis strain bacterial solution, and inactivating; c, mixing the collected porcine streptococcus strain bacterial solution, the haemophilus parasuis strain bacterial solution and the haemophilus parasuis strain bacterial solution, adding Tween-80 for preparing a water phase, preparing white oil, Span-80 and aluminium stearate into an oil phase, mixing the water phase with the oil phase to prepare a uniform emulsion, i.e. an oil emulsion inactivating vaccine; and 4, sub-packaging the oil emulsion inactivating vaccine. The porcine streptococcus disease and haemophilus parasuis disease combined inactivate vaccine can effectively prevent the porcine streptococcus disease and haemophilus parasuis disease, does not have hidden danger of scattering viruses and is safe and reliable; and the immunization is realized by one vaccine, thus the cost is reduced.

Description

technical field [0001] The invention discloses a streptococcus suis (type 2) and Haemophilus parasuis disease (type 4, type 5) double inactivated vaccine, which belongs to the field of biotechnology, and more specifically relates to a double streptococcus suis and Haemophilus parasuis disease The inactivated vaccine also relates to a method for preparing a streptococcus suis and Haemophilus parasuis dual inactivated vaccine; the prepared streptococcus suis and Haemophilus parasuis dual inactivated vaccine is mainly suitable for sows, Boars and piglets. technical background [0002] Both Streptococcus suis and Haemophilus parasuis are conditional pathogenic bacteria, which can easily lead to disease when the body's immunity is weakened. [0003] Streptococcus suis is an important pathogenic bacterium that causes streptococcal suis disease. It has 33 serotypes, among which Streptococcus suis type 2 can cause acute sepsis, meningoencephalitis, arthritis and acute death in pigs...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20A61K39/116A61P31/04C12R1/46C12R1/21A61K39/09A61K39/102
Inventor 徐高原康超周明光王杨波陈波陈章表陈关平蔡旭旺金梅林陈焕春
Owner WUHAN KEQIAN BIOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap