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Immunization method of chicken embryos

A chicken embryo and immunization technology, which is applied in application, poultry industry, animal husbandry, etc., can solve problems such as improper operation, chicken pollution, breeding loss, etc.

Inactive Publication Date: 2012-04-11
林继恒
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the existing method, due to improper operation, the chicks that are about to hatch will be polluted during the immunization process of chicken blanks, and then infected, resulting in the death of chicken embryos and causing losses to breeding.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 2

[0020] Specific embodiment 2: adopt 260 chicken embryos of 18-19 days old hatched at 38°C, take eggs in a dark room and draw the edge of the air chamber and the position of the embryo head, stand on the egg rack with the air chamber facing upwards, and sterilize the whole For the air chamber part, use a puncher to punch a pinhole 0.5cm above the edge of the air chamber on the side of the embryo head for immunization, then take a sufficient amount of solid paraffin and place it in a container, put it on the flame of an alcohol lamp and heat until the paraffin is completely In liquid state, use a small dropper to carefully absorb 1-2 drops of liquid paraffin and drop it on the needle hole to seal the hole, and then stand on the egg rack with the air chamber facing up to continue hatching.

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PUM

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Abstract

The invention discloses an immunization method of chicken embryos, comprising the following immunization steps of (1) sealing, steaming and disinfecting the immunization space with potassium permanganate and formalin in each cubic meter; (2) disinfecting the chicken embryo air-chamber with alcohol of which the concentration is 75%; (3) candling the chicken embryos of which the age is 18-19 days to screen the dead eggs or the unfertilized eggs; (4) diluting the immunization vaccine with 200-220 milliliters of diluent in each 4000 dose; (5) immunizing the single strain with the immunization chicken embryo with the diluted vaccine, wherein the immunization part is the air-chamber part of the chicken embryo; and (6) sealing, which is to seal the immunization needle holes of the chicken embryo eggs which are immunized. In the invention, the immunization space and the immunization parts are firstly disinfected, so the infection possibility is reduced, and the temperature and the humidity of the immunization space are stabilized; and because of the immunization sealing, the immunization effect is ensured, the stress death of the chicken embryos is prevented, and the hatch loss is reduced.

Description

Technical field: [0001] The invention relates to chicken embryo immunization technology, in particular to a chicken embryo immunization method. Background technique: [0002] Immunization of 18-19-day-old chicken embryos is one of the important methods of virus immunology. Viruses from poultry can be reproduced in chicken embryos, and mammalian viruses such as bluetongue virus and influenza virus can also be reproduced in chicken embryos. Proliferation in embryos, chicken embryo inoculation technology is considered to be the most classic and applicable method in influenza, especially avian influenza virus immunization methods. [0003] However, in the existing method, often due to improper operation, the chicken chick that is about to hatch will be polluted during the chicken blank immunization process, and then infected, causing the death of the chicken embryo and causing losses to the breeding. Invention content: [0004] The technical problem to be solved by the presen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K45/00
Inventor 林继恒
Owner 林继恒
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