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Application of swine fever and porcine pseudorabies live vaccine to preparation of medicament for treating or preventing swine fever and porcine pseudorabies

A technology for swine pseudorabies and swine fever live vaccine is applied in the application field of swine fever and swine pseudorabies live vaccine, which can solve the problems of reducing vaccine immune protection, interfering with vaccine antibodies, reducing vaccine immune effect, etc., and preventing porcine respiratory syndrome. , Guarantee immune effect, break through the effect of interference

Inactive Publication Date: 2015-01-21
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In addition, after swine fever vaccine and porcine pseudorabies vaccine are used to inoculate piglets by intramuscular route, in the presence of maternal antibodies, the virus will neutralize the protective antibodies and reduce the immune protection of the vaccines
When the immunization route is muscle immunization, it will interfere with the production of vaccine antibodies, thereby reducing the immune effect of the vaccine

Method used

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  • Application of swine fever and porcine pseudorabies live vaccine to preparation of medicament for treating or preventing swine fever and porcine pseudorabies
  • Application of swine fever and porcine pseudorabies live vaccine to preparation of medicament for treating or preventing swine fever and porcine pseudorabies
  • Application of swine fever and porcine pseudorabies live vaccine to preparation of medicament for treating or preventing swine fever and porcine pseudorabies

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation of embodiment 1 swine fever, porcine pseudorabies dual live vaccine

[0026] 1. Preparation of classical swine fever and porcine pseudorabies virus antigens

[0027] 1.1 Preparation of poisonous seeds for production

[0028] The preparation of the attenuated strain of classical swine fever rabbitization: the attenuated strain of classical swine fever rabbitization (purchased from the China Veterinary Drug Administration, the preservation number is AV1412) is properly diluted with the virus diluent (DMEM medium without serum), according to the multiplicity of infection ( M.O.I) was 0.1, inoculated on ST cell monolayer (purchased from CCTCC, No. GDC0060) and cultured, adsorbed at 37°C for 30 minutes, added DMEM cell maintenance solution containing 3% (v / v) calf serum, and cultured at 37°C for 3 to 5 days , freeze and thaw 2 to 3 times, harvest virus, virus titer ≥ 10 6.5 TCID 50 / ml.

[0029] Preparation of porcine pseudorabies virus gene deletion stra...

Embodiment 2

[0046] Example 2 The safety test of swine fever and porcine pseudorabies live vaccine in different immunization modes on piglets

[0047] 1. Materials

[0048] Live swine fever vaccine (rabbit attenuated strain) and porcine pseudorabies live vaccine (Bartha-K61 strain) were purchased from Pulaike Bioengineering Co., Ltd. (batch numbers 1207035 and 1202001B respectively). Vaccine (batch number is trial Z001) is prepared for embodiment 1.

[0049] 2. Design of animal experiments

[0050] Select 105 piglets at the age of 3 days after birth and divide them into 5 groups. Groups 1 to 4 are the test groups, 25 piglets per group. The piglets in the first group are inoculated with 2.0ml of live swine fever vaccine (2 piglets / head), the right side of the nasal cavity was inoculated with 2.0ml of live porcine pseudorabies vaccine (2 parts / head); the piglets of the second group were intramuscularly injected with 2.0ml of live swine fever vaccine (2 parts / head) in the left neck, and th...

Embodiment 3

[0058] Example 3 Test of protective effect on piglets after early nasal drip immunization of swine fever and porcine pseudorabies live vaccine

[0059] 1. Materials

[0060] Live swine fever vaccine and porcine pseudorabies live vaccine were purchased from Pulaike Bioengineering Co., Ltd. (batch numbers 1207035 and 1202001B, respectively). The real-time fluorescent RT-PCR detection kit for wild strain of classical swine fever virus and the real-time fluorescent PCR detection kit for porcine pseudorabies virus (gE gene) were purchased from Beijing Century Yuanheng Animal Epidemic Prevention Technology Co., Ltd. The swine fever, porcine pseudorabies dual live vaccine (batch number is trial Z001) is prepared for embodiment 1.

[0061] 2. Design of animal experiments

[0062] Select 100 piglets at the age of 3 days after birth and divide them into 4 groups, 25 piglets per group. The piglets in the first group were inoculated with 1.0ml of live swine fever vaccine (1 dose / head) i...

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Abstract

The invention provides application of swine fever and porcine pseudorabies live vaccines to preparation of medicaments for treating or preventing swine fever and porcine pseudorabies. The swine fever vaccine and porcine pseudorabies vaccine are used for immunization of piglets through nasal inhalation. The swine fever and porcine pseudorabies live vaccines can effectively prevent swine fever virus and porcine pseudorabies of piglets, at the same time significantly reduce the occurrence of porcine respiratory disease, and effectively prevent respiratory syndromes.

Description

technical field [0001] The invention relates to the application of live vaccines of swine fever and porcine pseudorabies. Background technique [0002] Classical Swine fever (CSF for short), also known as hog cholera (Hog cholera), is a viral infectious disease with acute or hyperacute fever in pigs. It is clinically characterized by rapid spread and fever. See typical hemorrhagic lesions. Swine fever can infect pigs of all ages and is prevalent throughout the year with high morbidity and mortality, which is extremely harmful to pigs. The disease is one of the most important infectious diseases that threaten the pig industry. The World Organization for Animal Health (OIE) lists swine fever as one of the 16 legal infectious diseases of Class A, and China defines it as a severe infectious disease. Porcine pseudorabies (Pseudorabies, PR) is an infectious disease common to a variety of animals. It is an infectious disease that can cause acute fatality in livestock and wild ani...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/245A61P31/22A61P31/14A61P11/00A61K39/187
Inventor 张许科孙进忠田克恭
Owner PU LIKE BIO ENG
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