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Preparation process and application of genetic engineering subunit vaccine of infectious bursal disease

A technology for bursal disease and genetic engineering, applied in the field of genetic engineering, can solve the problems that the expression of IBDVVP2 protein in Escherichia coli has no immunogenicity and insoluble inclusion bodies cannot be used

Active Publication Date: 2006-04-05
YEBIO BIOENG OF QINGDAO
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AI Technical Summary

Problems solved by technology

Research on the development of subunit vaccines and live bacterial vector vaccines using E. coli expression systems has also been carried out for many years, but the known research results are that E. coli expressing IBDV VP2 protein has no immunogenicity or forms insoluble inclusion bodies and cannot be used as a vaccine

Method used

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Embodiment Construction

[0136] The VP2 gene encoding the super-virulent strain of IBDV was connected to the pET-28a plasmid to form the expression plasmid pET-28a-VP2, which was transformed into Escherichia coli BL21 to obtain the genetically engineered strain Escherichia coliBL21 / pET-28-VP2. The strain can be used in the production of genetically engineered subunit vaccines, providing a new method for the prevention of IBD. The strain can also be used for the production of VP2 genetic engineering antigen and for the detection of chicken serum anti-IBD antibody.

[0137] gene sequence

[0138] Organization Applicant

[0139] City: Jingzhou, Hubei

[0140] Country: China

[0141] Postal Code: 434025

[0142] PhoneNumber: 07168496979

[0143] EmailAddress: rongjunl@public.js.hb.cn

[0144] OrganizationName: Yangtze University

[0145] Title: Preparation method and application of a genetically engineered subunit vaccine for infectious bursal disease

[0146] AppFileReferenc...

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PUM

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Abstract

The invention relates to a bacterium for the production of genetically-engineered subunit oil-adjuvant vaccines against infectious bursal disease, i.e. Escherichia coli BL21 / pET-28a-VP2, CCTCC No: M204038. The process for constructing the bacterium consists of inserting VP2 protein gene of the infectious bursal disease virus onto expression plasmid pET-28a to construct prokaryotic expression plasmid, then using the prokaryotic expression plasmid to transform the escherichia coli BL21, using lactose as inducer to induce Escherichia coli BL21 / pET-28a-VP2 expression VP2 protein, purifying and charging oil-adjuvant to obtain the vaccines.

Description

technical field [0001] The invention relates to a preparation method and application of a genetic engineering subunit vaccine for chicken infectious bursal disease, belonging to the field of genetic engineering. Background technique [0002] Infectious bursal disease (IBD) is an acute, highly contagious viral disease. It is an immunosuppressive infectious disease of chickens caused by infectious bursal disease virus (IBDV). IBD is highly contagious and can spread rapidly within a flock. [0003] The causative agent of the disease, IBDV, is highly resistant to inactivation. Therefore, the use of vaccines is necessary and mandatory in addition to strict hygienic measures in situations of high infectious pressure. The main purpose of this is to protect the chicks from IBDV infection in the first few weeks after hatch. There are two important measures to achieve this goal. First, high-titer maternal antibodies should be induced by inactivated oil emulsion vaccines throughout...

Claims

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Application Information

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IPC IPC(8): C12N1/21C12N15/33C07K14/005C07K14/18A61K39/12G01N33/53
Inventor 荣俊程太平刘晓娜杨待建
Owner YEBIO BIOENG OF QINGDAO
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