Process of producing gene engineering subunit vaccine of chicken's infectious bursal disease with colibacillus

A subunit vaccine, bursal disease technology, applied in the field of genetic engineering, can solve the problem of high production cost

Inactive Publication Date: 2006-10-18
YANGTZE UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This process requires the slaughter of a large number of chickens and is therefore expensive to produce

Method used

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Embodiment Construction

[0048] The VP2 gene encoding the super-virulent strain of IBDV was connected to the pBV220 plasmid to form the expression plasmid pBV220-VP2, which was transformed into Escherichia coli BL21 to obtain the genetically engineered strain Escherichia coliBL21 / pBV220-VP2. The strain can be used in the production of genetically engineered subunit vaccines, providing a new method for the prevention of IBD. The strain can also be used to produce VP2 genetic engineering antigen, which can be used to detect anti-IBD antibody in chicken serum.

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Abstract

The present invention relates to one kind of bacillus strain for producing gene engineering subunit vaccine of chicken's infection bursal disease and corresponding production process. The bacillus spawn is Escherichia coli BL21 / pBV220-VP2,CCTCC NO:M204037, and is constituted through cloning VP2 gene from the separated virulent strain of chicken's infection bursal disease, inserting into pBV220 plasmid to constitute pronucleus expressing plasmid, transforming colibacillus BL21 spawn with the pronucleus expressing plasmid, hot inducing for the Escherichia coli BL21 / pBV220-VP2 spawn to express VP2 protein, purifying properly, and adding into oil emulsion to prepare oil emulsion vaccine. The vaccine is used for meat chicken, laying hen and breeding cock to prevent chicken's infection bursal disease.

Description

Technical field: [0001] The invention relates to a method for producing chicken infectious bursal disease genetic engineering subunit vaccine by using Escherichia coli, which belongs to the field of genetic engineering. Background technique: [0002] Infectious bursal disease (Infectious bursal disease, IBD) is an immunosuppressive infectious disease of chickens caused by infectious bursal virus (Infectious bursal disease virus, IBDV). spread rapidly in the group. [0003] At present, there are two main ways to prevent this disease: one is to apply a moderately virulent live vaccine that is not fully attenuated, and its main purpose is to provide a strong enough immune stimulus to enable the immunized chickens to produce protection against virulent and super-virulent strains effect. However, these moderately virulent live vaccines may themselves cause immunosuppression and interfere with the immune effects of other vaccines. Another effective method is to use an inactivat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/21A61K39/12A61P31/14G01N33/53
Inventor 荣俊程太平刘晓娜杨待建
Owner YANGTZE UNIVERSITY
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