Method for preparing tetanus toxin recombinant antigen and its use

A technology of tetanus toxin and tetanus, applied in the field of genetic engineering, can solve the problems of low recovery rate, complicated steps of C fragment, high toxicity, etc., and achieve the effects of good immunogenicity, easy reproduction, and prevention of hydrolysis
CN1641034AInactive Publication Date: 2005-07-20NAT INST FOR THE CONTROL OF PHARMA & BIOLOGICAL PROD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NAT INST FOR THE CONTROL OF PHARMA & BIOLOGICAL PROD
Publication Date
2005-07-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to the recombinant vector of tetanus toxin antigen protein gene, the vector transformed host, tetanus toxin antigen produced with the transformant, and the application of the antigen protein in tetanus vaccine and conjugated vaccine protein vector. The recombinant plasmid vector of the present invention contains tetanus toxin antigen protein gene and is constituted via connecting tetanus toxin antigen protein gene and prokaryotic expression vector pET-22b(+). The transformant is obtained via transforming colibucillus with the recombinant plasmid. The expression system of the present invention has high efficiency expression of tetanus toxin antigen protein with expression amount of 15 %, and the purified expression protein has purity up to 96.5%. The tetanus toxin antigen of the present invention high immunogenicity, and may be used in preparing tetanus vaccine and the vector protein of conjugated vaccine.
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Description

technical field

[0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a recombinant vector containing a tetanus toxin antigen gene, a host transformed with the vector and a transformant used to produce the tetanus toxin antigen, and the antigen in a tetanus vaccine and a combination vaccine protein carrier in the application. technical background

[0002] Tetanus is caused by the infection of Clostridium tetani (Clostridium tetani). Under anaerobic conditions, bacteria produce a large amount of toxin-tetanus neurotoxin (abbreviated as tetanus toxin). Sexual muscle tonic spasm, severe cases finally died of suffocation and systemic failure. It is generally estimated that about 1 million people die from tetanus each year, about 80% of whom are newborns. Due to its sinister symptoms, high morbidity and mortality, tetanus has been highly valued by the medical profession. The most effective way to control tetanus is to vaccinat...

Claims

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