Pig breeding and respiratory syndrome NVDC-JXA1 strain-porcine parvovirus disease duplex inactivated vaccine and preparation method and application thereof

Abstract

The invention particularly relates to a pig breeding and respiratory syndrome NVDC-JXA1 strain-porcine parvovirus disease duplex inactivated vaccine and a preparation method and application thereof. The pig breeding and respiratory syndrome NVDC-JXA1 strain-porcine parvovirus disease duplex inactivated vaccine is prepared by enabling pig breeding and respiratory syndrome virus NVDC-JXA1 strain to inoculate with a Marc-145 cell, enabling a porcine parvovirus virulent strain to inoculate with an ST cell, respectively obtaining infection cell culture fluid to be inactivated by using formaldehyde solution, and conducting ultrafiltration, concentration and mixing to obtain mixed virus liquid to be mixed and emulsified with oil adjuvant. The inactivated vaccine comprises, by weight, 1-2 parts of the oil adjuvant and 1 part of mixed virus liquid aqueous phase, the inactivated vaccine is prepared by preparing pig breeding and respiratory syndrome virus liquid, porcine parvovirus liquid, an oil phase, the aqueous phase and emulsifying, and NVDC-JXA1 strain pig breeding and respiratory syndrome-porcine parvovirus disease duplex inactivated vaccine quality standards are built.

Description

[0001] technical field [0002] The invention relates to the technical field of veterinary biological products, in particular to a porcine reproductive and respiratory syndrome NVDC-JXA1 strain-porcine parvovirus dual inactivated vaccine and a preparation method and application thereof. [0003] Background technique [0004] Porcine reproductive and respiratory syndrome (PRRS), also known as blue ear disease, is a highly contagious infectious disease of pigs caused by porcine reproductive and respiratory syndrome virus (PRRSV). Infection, but pregnant sows and piglets under 1 month of age are most susceptible. The disease is mainly characterized by miscarriage, stillbirth, weak fetus, mummified fetus, dyspnea, sepsis and high mortality in piglets. Since there is no specific drug for the treatment of the disease, the use of specific vaccines has become the main measure for the prevention and control of PRRS. At present, two types of vaccines, inactivated vaccine and attenu...

AI Technical Summary

Problems solved by technology

Because there is only a single vaccine available on the market at present, to prevent these two diseases at the same time, pigs of the same age group often need to be immunized with 2 injections, which increases the immune density of pigs of each age group, while the cost of vaccines and the cost of injection Manpower and stress on pigs, as well as short-term high-density injections cause mutual interference between vaccines, making pig farm operators miserable

With the development of my country's intensive pig industry, traditional monovalent vaccines can no longer meet the needs of immunity, and there is an urgent need for a vaccine that can prevent multiple diseases with one shot; therefore, in order to meet the epidemic prevention needs of the pig industry, a safe, stable and capable Porcine Reproductive and Respiratory Syndrome-Porcine Parvovirus Dual Inactivated Vaccine with "One Injection to Prevent Two Diseases" is Urgent and Important