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Water-in-oil-in-water adjuvant vaccine and preparation method thereof

A water-in-oil-in-water and adjuvant technology, which is applied in the field of vaccines, can solve the problems of adverse effects on animal carcass quality, difficulty in providing protective antibodies, and slow speed of protective antibodies, achieving rapid immune protection and good local absorption by injection. , the effect of maximizing bioavailability

Active Publication Date: 2013-07-31
SOUTH CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the production technology of domestic and foreign vaccine manufacturers has been continuously improved, and the quality of the oil used has also been continuously improved, but because the continuous oil phase of this preparation tends to reside at the injection site, in many cases, this W / O type Oil adjuvant vaccines produce a large number of sterile abscesses and granulomas near the vaccination site, the production of protective antibodies is slow, it is easy to cause adverse effects on animal carcass quality, and may cause severe pain during vaccination
[0004] Oil-in-water (O / W) adjuvanted vaccines have the advantages of small local reactions at the injection site and high stability. However, the antigens contained or exposed in the continuous phase quickly disperse at the injection site and are decomposed by body fluid enzymes, making it difficult to provide Long-term protective antibodies often require special immunostimulators and multiple booster immunizations, which are expensive to prepare and not widely used

Method used

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  • Water-in-oil-in-water adjuvant vaccine and preparation method thereof
  • Water-in-oil-in-water adjuvant vaccine and preparation method thereof
  • Water-in-oil-in-water adjuvant vaccine and preparation method thereof

Examples

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Effect test

Embodiment 1

[0039] A water-in-oil-in-water type adjuvanted vaccine, which is composed of an internal water phase, an intermediate oil phase for coating the internal water phase, and an intermediate oil phase for coating the internal water phase;

[0040] The components in the external water phase, based on the total volume of the water-in-oil-in-water type adjuvant vaccine raw materials, the ratio is: compound surfactant 1%, Tween-80 0.3%, physiological saline 15.5%;

[0041] The components in the middle oil phase, based on the total volume of the water-in-oil-in-water type adjuvant vaccine raw materials, the ratio is: span 80 3%, aluminum hardate 8.5 g / L, imported white oil 47%;

[0042]The components in the internal water phase, based on the total volume of the water-in-oil-in-water type adjuvant vaccine raw materials, the ratio is: 0.15g / mL astragalus polysaccharide injection 2%, Tween-80 1.2%, and the balance is Live antigen solution; the total volume percentage of the water-in-oil-in...

Embodiment 2

[0050] A water-in-oil-in-water type adjuvanted vaccine, which is composed of an internal water phase, an intermediate oil phase for coating the internal water phase, and an intermediate oil phase for coating the internal water phase;

[0051] The components in the external water phase, based on the total volume of the water-in-oil-in-water type adjuvant vaccine raw materials, the ratio is: 0.5% compound surfactant, 0.6% Tween-20, 18% normal saline;

[0052] The components in the middle oil phase, based on the total volume of the water-in-oil-in-water type adjuvant vaccine raw materials, the ratio is: span 60 2%, aluminum hardate 10 g / L, imported white oil 30%;

[0053] The components in the internal water phase, based on the total volume of the water-in-oil-in-water type adjuvant vaccine raw materials, the ratio is: 0.1g / mL astragalus polysaccharide injection 1.5%, Tween-80 0.5%, the balance is Live antigen solution; the total volume percentage of the water-in-oil-in-water typ...

Embodiment 3

[0061] A water-in-oil-in-water type adjuvanted vaccine, which is composed of an internal water phase, an intermediate oil phase for coating the internal water phase, and an intermediate oil phase for coating the internal water phase;

[0062] The components in the external water phase, based on the total volume of the water-in-oil-in-water type adjuvant vaccine raw materials, the ratio is: compound surfactant 1.51%, Tween-60 0.5%, physiological saline 9%;

[0063] The components in the middle oil phase, based on the total volume of the water-in-oil-in-water type adjuvant vaccine raw materials, the ratio is: span 80 4%, aluminum hardate 5 g / L, imported white oil 50%;

[0064] The components in the internal water phase, based on the total volume of the water-in-oil-in-water type adjuvant vaccine raw materials, the ratio is: 0.13g / mL astragalus polysaccharide powder aqueous solution 2.5%, Tween-20 1.5%, the balance is Live antigen solution; the total volume percentage of the wate...

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Abstract

The invention discloses a water-in-oil-in-water adjuvant vaccine and a preparation method thereof. The adjuvant vaccine is composed of an internal water phase, a middle oil phase used for cladding the internal water phase, and an external water phase for cladding the middle oil phase. The external water phase comprises: a composite surfactant, a hydrophilic surfactant and mormal saline. The middle oil phase consists of: a lipophilic surfactant, a stabilizer and mineral oil. The internal water phase comprises: an immunopotentiator solution, the hydrophilic surfactant, and an inactivated antigen solution. The W / O / W (water-in-oil-in-water) type adjuvant vaccine provided in the invention can induce an immunoreaction earlier than traditional W / O (water-in-oil) type adjuvant vaccines, and can make experimental animals obtain earlier protective antibodies. The vaccine has good absorption, and causes a small inflammatory response at an inoculation site. The adjuvant vaccine disclosed in the invention has better stability than existing W / O / W type vaccines, and the antibody generation speed is fast.

Description

technical field [0001] The invention relates to the field of vaccines, in particular to a water-in-oil-in-water adjuvant vaccine and a preparation method thereof. Background technique [0002] When an inactivated antigen is used alone to immunize animals, it is not sufficient to produce immunogenicity, especially for antigens with low immunogenicity and small molecular weight. Therefore, different kinds of adjuvants are needed to enhance the immune response induced by the antigen. The oil adjuvant vaccines promoted and applied by veterinary vaccine manufacturers at home and abroad contain a large amount of mineral oil, which not only protects the poultry industry, but also brings many food safety problems. For example, the problem of vaccine residues in broiler carcasses has caused increasing concern. [0003] Water-in-oil (W / O) oil adjuvant vaccine is prepared by mixing antigen solution, stabilizer and mineral oil under high shear force. The immune mechanism is that it i...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K39/00A61P31/00
Inventor 罗开健张光明叶贺佳梁昭平李敏王斌朱辉
Owner SOUTH CHINA AGRI UNIV
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