Porcine parvovirus L strain and use thereof in preparation of porcine parvovirus inactivated vaccines

A parvovirus, inactivated vaccine technology, used in antiviral agents, viruses/phages, biochemical equipment and methods, etc.

Active Publication Date: 2010-10-06
哈药集团生物疫苗有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the application of porcine parvovirus inactivated by diethyleneimine, and adding oil adjuvant to prepare a two-way oil emulsion inactivated vaccine, it has not been reported yet

Method used

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  • Porcine parvovirus L strain and use thereof in preparation of porcine parvovirus inactivated vaccines
  • Porcine parvovirus L strain and use thereof in preparation of porcine parvovirus inactivated vaccines
  • Porcine parvovirus L strain and use thereof in preparation of porcine parvovirus inactivated vaccines

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Experimental program
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Effect test

Embodiment 1

[0027] The present invention will be further described below in conjunction with specific embodiments, and the advantages and characteristics of the present invention will become clearer along with the description. However, these embodiments are only exemplary and do not constitute any limitation to the scope of the present invention. Those skilled in the art should understand that the details and forms of the technical solutions of the present invention can be modified or replaced without departing from the spirit and scope of the present invention, but these modifications and replacements all fall within the protection scope of the present invention. Example 1 Isolation, cultivation and identification of porcine parvovirus L strain

[0028] 1. Breeding method

[0029] 1. Collection of disease materials: Aseptically collect the liver, mesenteric lymph nodes, kidneys, brain and other tissues of stillborn sows. Add sterilized saline at 1:10, grind to prepare tissue suspension...

Embodiment 2

[0052] Example 2 Preparation of Porcine Parvovirus Inactivated Vaccine

[0053] 1. Preparation of virus liquid for seedling production: insert 0.5% of the virus culture liquid into the ST cell culture that has not yet formed a monolayer for the production of seed virus, put 37 ° C for rotary culture, and when the lesion reaches 70%, harvest the virus containing Toxic cell culture medium, after 3 times of freezing and thawing, the poison was collected.

[0054] 2. Inactivation: 0.02% of the total amount of virus liquid was added to the virus liquid with divinylimine solution, shaken fully, inactivated on a shaker at 30°C and 100r / min for 72 hours, and then added 1% sodium thiosulfate solution , to terminate the inactivation. And for sterility test.

[0055] 3. Concentration: Take the inactivated virus liquid, centrifuge continuously at 13000r / min, and then filter with a 0.2um membrane filter. The virus liquid after microfiltration was concentrated 5 times by ultrafiltration ...

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Abstract

The invention discloses a porcine parvovirus L strain and use thereof in preparation of porcine parvovirus inactivated vaccines. In the invention, microbial preservation number of the separated strain is CGMCC No. 3352. The strain is highly homologous with an NADL-2 strain and a China strain and keeps high reproduction, and the viruses are stable. Oil adjuvant is added into antigen liquid which inactivates the viruses by using binary ethylenimine to prepare the bidirectional oil-emulsion inactivated vaccines; and the prepared inactivated vaccines are applied in a vaccine inoculation experiment of 10,276 pigs (most of which are first farrowing sows), and in the experiment, an immune dosage of 2 millimeters is given to each pig through muscle injection, and each time of inactivated vaccine inoculation can obtain an immune period of over 6 months. Under the conditions that a 10 millimeter overdose vaccine is injected into a pig and that single dose inoculation is repeatedly carried out for three times, no abnormal reaction happens, which proves that the inactivated vaccines have high immunogenicity and safety.

Description

technical field [0001] The present invention relates to a newly isolated virus strain, in particular to a porcine parvovirus L strain. The invention also relates to the use of the virus L strain in preparing porcine parvovirus disease inactivated vaccine, which belongs to the prevention and treatment of porcine parvovirus disease field. Background technique [0002] Porcine parvovirus (PPV) can cause reproductive disorders in pigs, mainly manifested as infection and death of fetuses and embryos, while the mother usually does not show clinical symptoms. Serologically negative sows are primarily infected via the oronasal route during the first half of gestation, with consequent transplacental infection of immunocompromised fetuses leading to disease. The virus is ubiquitous in pig herds around the world and endemic in most farms. The disease is very popular and has been reported in many countries in Europe, America, Asia and Oceania. Porcine parvovirus has been isolated in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A61K39/23A61P31/20
Inventor 吴金任德强柴华戴秀莉张立恒张智明杨万秋藏玉婷胡瑛瑛崔艳丽潘兴广
Owner 哈药集团生物疫苗有限公司
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