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Method for collecting earthworms by utilizing sasangua cake solution

A technology of tea and solution, applied in the fields of botanical equipment and methods, chemicals for biological control, applications, etc., can solve the problems of difficult standardization, difficult to determine the exact dose, soil biological toxicity, etc., and achieve easy degradation and sampling. Good effect and environmentally friendly effect

Inactive Publication Date: 2009-12-09
GUANGDONG ENTOMOLOGICAL INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Formalin is the most commonly used chemical agent for earthworm sampling. The sampling efficiency is high and the method is easy to standardize. However, recent studies have shown that formalin has persistent toxicity to soil organisms and surface plants. Therefore, it should not be used in ecological farms, conservation areas, or areas of soil that may infiltrate into groundwater
There are also many research applications on the use of mustard for earthworm sampling. There have been suggestions to replace the formalin method, but the sampling efficiency of the mustard method is not stable enough, and the commercial mustard components and proportions are not clear, so it is difficult to standardize
In recent years, there have been some reports on the use of allyl isothiocyanate (AITC) for earthworm sampling. Allyl isothiocyanate is a glucosinolate that can be extracted from many cruciferous plants. It has a pungent mustard smell and is slightly soluble in water. , easy to decompose when exposed to light, and its half-life in soil is 80-120h. Its sampling efficiency is similar to that of formalin method, but even if allyl isothiocyanate is stored in acetone (acetone) solution protected from light, it will Evaporates rapidly from the solution interface, and its exact dosage is difficult to determine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] In May 2007, samples were selected in the grass in the Institute of Entomology of Guangdong Province. Chaku powder was added to water at a mass ratio of 1:3, heated to reflux for 1 hour, and then filtered to obtain Chaku filtrate. According to the mass ratio of 1:8, the tea dry filter solution is added into water to dilute to prepare a tea dry solution, and the tea dry solution is continuously sprayed on the selected soil sample square. A total of 3 sample plots were selected, each with 6 small sample plots, a total of 18 1 / 4m 2 The sampling area of ​​each plot is 1.5m 2 . 1 / 4m each 2 Spray 5000ml of tea dry solution on the sample quadrat. During the spraying process, earthworms crawled out. Collect the crawled earthworms until no earthworms crawled out. Wait for about 1 hour for the earthworms to crawl out. After 1 hour, dig the sample quadrat by hand. Count the number of earthworms that have not been driven out, and calculate the driving and collection efficiency o...

Embodiment 2

[0020] In May 2007, samples were selected in the grass in the Institute of Entomology of Guangdong Province. Chaku powder was added to water at a mass ratio of 1:3, heated to reflux for 0.5 h, and then filtered to obtain Chaku filtrate. Add the tea-cured filtrate to water to dilute according to the mass ratio of 1:5 to prepare a tea-cured solution, and continuously spray the tea-cured solution on the selected soil sample squares. A total of 3 plots were selected, each with 6 small plots, and 18 plots of 1 / 4m 2 The sampling area of ​​each plot is 1.5m 2 . 1 / 4m each 2 Spray 7000ml of diluted tea dead solution on the sample quadrat. During the spraying process, earthworms crawled out. Collect the crawled earthworms until no earthworms crawled out. Wait for about 1 hour for the earthworms to crawl out. After 1 hour, dig the sample quadrat by hand. , count the number of earthworms that have not been driven out, and calculate the driving and collection efficiency of the tea dead ...

Embodiment 3

[0024] In May 2007, samples were selected in the grass of the Guangdong Provincial Institute of Entomology. The selected tea dry powder was added to the water at a mass ratio of 1:3, heated to reflux for 2 hours, and then filtered to obtain the tea dry filter solution. Add the tea Ku filtrate to water to dilute according to the mass ratio of 1:9, prepare the Cha Ku solution, and continuously spray the Cha Ku solution on the selected soil sample squares. A total of 3 plots were selected, each with 6 small plots, and 18 plots of 1 / 4m 2 The sampling area of ​​each plot is 1.5m 2 . 1 / 4m each 2 Spray 9000ml of diluted tea dead solution on the sample quadrat. Earthworms crawl out during the spraying process. Collect the crawled earthworms until no earthworms crawl out. Wait for about 1 hour for the earthworms to crawl out. After 1 hour, dig the sample quadrat by hand. , count the number of earthworms that have not been driven out, and calculate the driving and collection efficien...

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Abstract

The invention discloses a method for collecting earthworms by utilizing sasangua cake solution, aiming to provide a method for collecting earthworms with the characteristics of no residual contamination, environmental friendliness, good sampling results, and stable sampling efficiency. The method comprises the following steps: (a): the preparation of the sasangua cake solution is as follows: sasangua cake powder is added into water in ratio of 1:3 by weight, the mixture is boiled by boiling water for 0.5-2 hours, and then is filtered to obtain sasangua cake filtered fluid, the obtained sasangua cake filtered fluid is added into water in ratio of 1:5-9 by weight to prepare the sasangua cake solution; (b): the sasangua cake solution is evenly sprayed on soil samples, with 5-9 litres of the sasangua cake solution on 0.25 square meters, to collect earthworms climbing out. The method is particularly suitable for collecting earthworms in ecological farms, conservation district, or soil region into which ground water may permeate.

Description

Technical field: [0001] The invention relates to a method for collecting earthworms, in particular to a method for collecting earthworms in the field by using tea dead solution. Background technique: [0002] Earthworms are the most important large soil animals in terrestrial ecosystems, which have a very positive impact on the transformation of soil organic matter and the establishment of soil structure. At the same time, earthworms are extremely sensitive to the soil environment, and their community distribution, species composition and density can be used as important indicators of soil conditions in a region. Therefore, the community survey of earthworms has been widely used in the evaluation of soil ecosystem effects. However, the field sampling methods of earthworms have not yet established a unified and perfect standard, and various sampling methods also have certain shortcomings. At present, the methods used in earthworm sampling include physical methods such as ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01M5/00A01N65/08A01P17/00
Inventor 龚鹏博李建雄张卫信廖崇惠
Owner GUANGDONG ENTOMOLOGICAL INST
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