High temperature resistant Issatchenkia orientalis, application thereof and method for producing ethanol through fermentation

An Isaac yeast and high-temperature fermentation technology, applied in the field of fermentation, can solve the problems of reduced ethanol fermentation capacity, ethanol tolerance, inability to perform the fermentation process normally, and unsuitable for industrial production, and achieves the convenience of industrial production, reduction of fermentation costs, and reduction of energy consumption. Effect

Inactive Publication Date: 2012-11-28
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A further increase in temperature will lead to the aging and death of yeast, which will reduce its ethanol fermentation ability and tolerance to ethanol. The fermentation process cannot be carried out normally, and the production efficiency is low, which is not suitable for industrial production.
Therefore, it is necessary to take a series of cooling measures in production, such as spraying with deep well water, etc., which is very expensive, especially in the high temperature season of summer, it is more difficult to control the temperature

Method used

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  • High temperature resistant Issatchenkia orientalis, application thereof and method for producing ethanol through fermentation
  • High temperature resistant Issatchenkia orientalis, application thereof and method for producing ethanol through fermentation

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1 Screening of heat-resistant Isa orientalis IPE100

[0028] Isolation and screening of IPE100: collect samples from the waste of farmland crops (corn stalks) in Daxing, Beijing, and activate the collected samples, take 1g of them and add 100mL to YPG medium supplemented with ampicillin, 250mL Erlenmeyer shaker Shake culture, 42°C, 150rpm. After 48 hours of enrichment culture, the culture solution was diluted in a certain gradient and spread on the solid plate of the same medium. After culturing at 42°C for 48 hours, a single colony of typical yeast form was picked, and then repeated on the solid plate of the same medium. After line purification three times, store at 4°C. Then the ethanol yield and fermentation cycle were determined by ethanol fermentation experiments at different temperatures, and the inhibitor degradation test was used to detect the ability of the strains to detoxify the inhibitors. Finally, the strains with better effects were selected accordin...

Embodiment 2

[0035] Example 2 High temperature ethanol fermentation of lignocellulosic hydrolysate using the heat-resistant Isa orientalis IPE100 of the present invention

[0036] Using liquid seed culture medium, the thermostable Isa orientalis IPE100 was cultured in a shake flask at 42°C and 150 rpm for 16 hours to obtain liquid seeds. The liquid seeds were inoculated to the (NH) 4 ) 2 SO 4 1g / L, MgSO 4 0.02g / L, K 2 HPO 4 0.03g / L, lignocellulose hydrolysate, pH 6.0 fermentation medium, 42°C, 150rpm culture for 48h, the ethanol yield reached the maximum value, 93.8% of the theoretical value (0.51g / g). The composition of the lignocellulose hydrolysate used is as follows: glucose 95.70g / L, furfural 0.05g / L, 5-hydroxymethylfurfural 0.15g / L.

Embodiment 3

[0037] Example 3 High temperature ethanol fermentation of lignocellulosic hydrolysate using the heat-resistant Isa orientalis IPE100 of the present invention

[0038] Using liquid seed culture medium, the thermostable Isa orientalis IPE100 was cultured in a shake flask at 42°C and 150 rpm for 16 hours to obtain liquid seeds. The liquid seeds were inoculated to the (NH) 4 ) 2 SO 4 1.2g / L, MgSO 4 0.03g / L, K 2 HPO 4 0.04g / L, lignocellulose hydrolysate, pH 7.0 fermentation medium, 38°C, 180rpm culture for 48h, the ethanol yield reached the maximum value, 90.3% of the theoretical value (0.51g / g). The composition of the lignocellulose hydrolysate used was the same as in Example 1.

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Abstract

The invention relates to the field of fermentation, in particular to a strain of high temperature resistant Issatchenkia orientalis, application thereof and a method for producing ethanol through fermentation. The strain of high temperature resistant Issatchenkia orientalis IPE100 is separated and screened from farmland crop waste, has a number of CGMCC (China General Microbiological Culture Collection Center) No.4927, has the capacity of fermenting ethanol at high temperature, and can be applied to high temperature fermentation of ethanol by using lignocelluloses hydrolysis products and hightemperature solid fermentation of ethanol by using sweet sorghum.

Description

Technical field [0001] The present invention relates to the field of fermentation, in particular, to a high-temperature resistant Isa orientalis strain, its application and a method for producing ethanol by fermentation. Background technique [0002] Due to the limited reserves of mineral energy and the increasing awareness of environmental protection, the use of renewable biomass for energy production has attracted more and more attention. Therefore, it is urgent to find a fuel that can guarantee the normal supply of energy and has low pollution. Ethanol is a renewable energy source and the most promising alternative to fossil fuels. The ethanol production method is mainly based on yeast fermentation. The optimum fermentation temperature for industrial yeast is 30℃~33℃, and generally should not exceed 36℃. A further increase in temperature will cause the aging and death of yeast, reduce its ethanol fermentation capacity and ethanol tolerance, the fermentation process cannot be...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12R1/645
CPCY02E50/16Y02E50/10
Inventor 刘春朝权永进王锋
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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