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High temperature resistant Issatchenkia orientalis, application thereof and method for producing ethanol through fermentation

An Isaac yeast and high-temperature fermentation technology, applied in the field of fermentation, can solve the problems of reduced ethanol fermentation capacity, ethanol tolerance, inability to perform the fermentation process normally, and unsuitable for industrial production, and achieves the convenience of industrial production, reduction of fermentation costs, and reduction of energy consumption. Effect

Inactive Publication Date: 2011-12-07
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A further increase in temperature will lead to the aging and death of yeast, which will reduce its ethanol fermentation ability and tolerance to ethanol. The fermentation process cannot be carried out normally, and the production efficiency is low, which is not suitable for industrial production.
Therefore, it is necessary to take a series of cooling measures in production, such as spraying with deep well water, etc., which is very expensive, especially in the high temperature season of summer, it is more difficult to control the temperature

Method used

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  • High temperature resistant Issatchenkia orientalis, application thereof and method for producing ethanol through fermentation
  • High temperature resistant Issatchenkia orientalis, application thereof and method for producing ethanol through fermentation

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1 Screening of high temperature resistant Issahia orientalis IPE100

[0028] Separation and screening of IPE100: collect samples from crop (corn stalk) waste in Daxing, Beijing, and activate the collected samples, take 1g of them and add 100mL to YPG medium supplemented with ampicillin, 250mL Erlenmeyer flask shaker Shaking culture, 42°C, 150rpm. After 48 hours of enrichment culture, the culture solution was diluted in a certain gradient, and spread on the solid plate of the same medium. After culturing at 42°C for 48 hours, a single colony of typical yeast form was picked, and then repeatedly streaked on the solid plate of the same medium. After three line purifications, they were stored at 4°C. Then, the ethanol yield and fermentation cycle were measured through ethanol fermentation experiments at different temperatures, and the detoxification ability of the strains to inhibitors was detected through inhibitor degradation experiments, and finally the strains ...

Embodiment 2

[0035] Example 2 Carry out high-temperature ethanol fermentation of lignocellulose hydrolyzate with the high-temperature-resistant Issahia orientalis IPE100 of the present invention

[0036] Using the liquid seed medium, the thermostable I. orientalis IPE100 was shaken at 42° C. and 150 rpm for 16 hours to obtain liquid seeds. The liquid seeds were inoculated to containing (NH 4 ) 2 SO 4 1g / L, MgSO 4 0.02g / L, K 2 HPO 4 0.03g / L, lignocellulose hydrolyzate, in the fermentation medium of pH 6.0, 42°C, 150rpm culture for 48h, the ethanol yield reached the maximum, which was 93.8% of the theoretical value (0.51g / g). The composition of lignocellulose hydrolyzate used is as follows: glucose 95.70g / L, furfural 0.05g / L, 5-hydroxymethylfurfural 0.15g / L.

Embodiment 3

[0037] Example 3 Carry out high-temperature ethanol fermentation of lignocellulose hydrolyzate with the high-temperature-resistant Issahia orientalis IPE100 of the present invention

[0038] Using the liquid seed medium, the thermostable I. orientalis IPE100 was shaken at 42° C. and 150 rpm for 16 hours to obtain liquid seeds. The liquid seeds were inoculated to containing (NH 4 ) 2 SO 4 1.2g / L, MgSO 4 0.03g / L, K 2 HPO 4 0.04g / L, lignocellulose hydrolyzate, in the fermentation medium of pH 7.0, 38°C, 180rpm for 48h, the ethanol yield reached the maximum, which was 90.3% of the theoretical value (0.51g / g). The composition of the lignocellulose hydrolyzate used is the same as in Example 1.

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Abstract

The invention relates to the field of fermentation, in particular to a strain of high temperature resistant Issatchenkia orientalis, application thereof and a method for producing ethanol through fermentation. The strain of high temperature resistant Issatchenkia orientalis IPE100 is separated and screened from farmland crop waste, has a number of CGMCC (China General Microbiological Culture Collection Center) No.4927, has the capacity of fermenting ethanol at high temperature, and can be applied to high temperature fermentation of ethanol by using lignocelluloses hydrolysis products and high temperature solid fermentation of ethanol by using sweet sorghum.

Description

technical field [0001] The invention relates to the field of fermentation, in particular to a strain of high-temperature resistant Issa orientalis, its application and a method for producing ethanol by fermentation. Background technique [0002] Due to the limited reserves of mineral energy and the enhancement of people's awareness of environmental protection, the use of renewable biomass for energy production has attracted more and more attention. Therefore, it is extremely urgent to find a fuel that can guarantee the normal supply of energy and has little pollution. Ethanol is a renewable energy source and the most promising alternative to fossil fuels. Yeast fermentation is the main method for ethanol production, and the optimum fermentation temperature for industrial yeast is between 30°C and 33°C, and generally should not exceed 36°C. A further increase in temperature will lead to the aging and death of yeast, which will reduce its ethanol fermentation ability and tol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P7/10C12R1/645
CPCY02E50/16Y02E50/10
Inventor 刘春朝权永进王锋
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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