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Methods for detoxifying a lignocellulosic hydrolysate

a technology of lignocellulosic hydrolysate and detoxification method, which is applied in the direction of cellulose treatment using microorganisms/enzymes, application, pulping with inorganic bases, etc., can solve the problems of significant drive up maintenance costs, and achieve the effect of minimising the loss of fermentable sugar

Inactive Publication Date: 2016-01-07
BP CORP NORTH AMERICA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present patent text describes a method for detoxifying hydrolysates obtained from lignocellulosic biomasses using magnesium bases. The detoxification process involves mixing a solution of the starting hydrolysate with a magnesium base and allowing it to react for a certain period of time at a specific temperature. The detoxified hydrolysate contains reduced levels of toxins that are harmful to fermenting microorganisms and minimal loss of fermentable sugars. This method offers advantages over other detoxification bases, such as calcium hydroxide, as it does not produce insoluble byproducts that cause fouling and pipeline clogging. The detoxification process can be carried out at temperatures between 40°C and 90°C and at a pH of between 6.5 and 8. The patent also describes a method for continuously producing a fermentation product by flowing a hydrolysate and a magnesium base through a continuous reactor and then fermenting it with a fermenting microorganism.

Problems solved by technology

Additionally, detoxification with magnesium bases does not result in the production of insoluble byproducts that cause fouling and pipeline clogging which significantly drive up maintenance costs.

Method used

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  • Methods for detoxifying a lignocellulosic hydrolysate
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  • Methods for detoxifying a lignocellulosic hydrolysate

Examples

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example 1

Hydrolysis of Lignocellulosic Biomasses

[0079]A lignocellulosic biomass (e.g., energy cane or sugar cane) was harvested and sized using a forage chopper, inoculated with a preparation of Lactobacillus bacteria and stored in agricultural bags until use. Prior to dilute acid hydrolysis, the lignocellulosic biomass was removed from bags and washed with process water to remove organic acids and then dewatered with a screw press. The biomass was then conveyed to a pressurized reaction chamber (i.e., hydrolyzer) along with water and sulfuric acid (0.2% to 3%). The liquid / solid ratio of the slurry was minimized to maximize the dissolved sugar concentration in the hydrolysate following hydrolysis. The retention time in the hydrolyzer and the temperature of the hydrolyzer was dependent on parameters of the biomass (e.g., moisture and glucan levels). In general, the temperature of the hydrolyzer ranged from 120° C. to 180° C. and the retention time ranged from 3 minutes to 2 hours.

[0080]Follow...

example 2

Detoxification of Hydrolysates with Magnesium Hydroxide—Batch Reactor

5.2.1. Materials and Methods

[0082]5.2.1.1. Sorghum Hydrolysate DP 100309

[0083]Hydrolysate DP 100309 was placed in a 1L three necked round bottom flask equipped with stir bars. The round bottom flask was placed in oil bath and heated to 70° C. While the hydrolysate solution was warming, target amounts of magnesium hydroxide slurry (i.e., supersaturated solution of magnesium hydroxide in water) were weighed. The total quantity of magnesium hydroxide added to the hydrolysate was determined from the titration of the hydrolysate solution with sodium hydroxide (see FIG. 3 and Example 6). Also, see Martinez et al., 2001, Biotechnol. Prog. 17(2):287-293. After the hydrolysate solution was heated to the desired temperature, the magnesium hydroxide slurry was added rapidly to the hydrolysate solution at a dosage of 22.86 g / Kg hydrolysate at 70° C. The solution was stirred vigorously with a magnetic stirrer for 3 hours. The p...

example 3

Detoxification of Hydrolysates—Series of CSTRS

[0097]The detoxification process is carried out using a series of continuously stirred tank reactors (CSTRs). The hydrolysate solution is heated to the target temperature with heating mantles and / or recirculating water bath and delivered by peristaltic pump to the first of a series of stirred reactors (1, 2, or 4L sizes) connected in series to which magnesium hydroxide slurry is previously added. The ratio of hydrolysate solution to magnesium hydroxide slurry added to the reactor is based on titration dosage (e.g., 1 ml / min of base slurry to 20 ml / min of hydrolysate). The resultant mixture is then pumped to one or more additional CSTRs. Additional base slurry is added to each successive CSTR reactor if the pH of the mixture fell below a certain level. Retention time in each reactor is constrained by fixing the target volume and maintaining a target flow rate (where rate multiplied by volume equals the retention time). To prevent build-up...

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Abstract

The present disclosure relates to methods for detoxifying a hydrolysate obtained from a lignocellulosic biomass and methods of producing ethanol from the detoxified hydrolysate. The present methods provide detoxified hydrolysates in which the quantity of compounds that are deleterious to fermenting microorganisms are substantially reduced relative to the starting hydrolysate and in which the amount of fermentable sugars loss is minimal.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is a divisional application of U.S. application Ser. No. 13 / 765,039 filed Feb. 12, 2013, now issued as U.S. Pat. No. 9,133,278; which claims the benefit under 35 USC §119(e) to U.S. Application Ser. No. 61 / 597,973 filed Feb. 13, 2012, now expired. The disclosure of each of the prior applications is considered part of and is incorporated by reference in the disclosure of this application.BACKGROUND OF THE INVENTION[0002]Many industrial products are produced by microorganisms grown in culture. Microorganism growth may be supported by soluble sugar molecules released by lignocellulosic biomasses. Lignocellulosic biomasses consist primarily of cellulose (polymers of glucose linked by β-1,4-glucosidic bonds), hemicellulose (polysaccharide composed of different five (C5)—carbon sugars and six (C6)—carbon sugars linked by variety of different β and α linkages) and lignin (complex polymer consisting of phenyl propane units linked...

Claims

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Application Information

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IPC IPC(8): C08B1/00C12P7/10
CPCC08B1/003C12P2201/00C12P7/10C12N1/22D21C1/06D21C3/02D21C5/005Y02E50/10C08B15/00
Inventor SLUPSKA, MALGORZATASATO, YUKIKOKUSTEDJO, KARENWONG, KELVIN
Owner BP CORP NORTH AMERICA INC
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