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Systems and methods for improving fermentation

A liquid and nanofiltration technology, applied in the field of systems and methods for improving fermentation processes, can solve the problems of reducing the level of pentose alcohol inhibitors, scaling of evaporation equipment and distillation equipment, and high cost performance

Inactive Publication Date: 2014-01-08
POET RES INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The combination of nanofiltration and addition of calcium hydroxide has not been used as calcium hydroxide is known to foul membranes and scale evaporation and distillation equipment
A system combining various inhibitor removal technologies may cost-effectively reduce inhibitor levels in pentose alcohols

Method used

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  • Systems and methods for improving fermentation
  • Systems and methods for improving fermentation
  • Systems and methods for improving fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] According to the experimental design shown in table 4A and as shown in the present embodiment Figure 9The experimental procedure shown in A was used for the acid removal experiment of the liquid component. The three different filters tested were: Dow Filmtec NF-4040, Dow Filmtec NF-270 (both available from Dow Chemical Company, Midland MI) and Koch SeIRO MPS-34 (available from Koch Membrane Systems, Inc., Wilmington, MA). All three filters utilize spiral-wound membranes with a diameter of 4 inches and a length of 40 inches. The filter operates at 25 degrees Celsius and the Dow Filmtec NF-270 operates at 32 degrees Celsius. A model of a multi-stage nanofiltration system such as Figure 9 As shown in the experimental flow for A, where the retentate 902 from the filter 904 can be recycled back into the storage / supply tank 906 and filtered again to simulate a second or consecutive filtration stage. The principle of concentration and diafiltration is as Figure 9 Shown...

Embodiment 2

[0083] According to the experimental design shown in table 5A and the present embodiment Figure 9 The experimental procedure shown in A was used for the acid removal experiment of the liquid component. Dow Filmtec NF filters (available from Dow Chemical Company, Midland MI) were used in this experiment. Dow Filmtec NF filters utilize a spiral-wound membrane that is 4 inches in diameter and 40 inches in length. The operating temperature of the filter is room temperature (approximately 22 degrees Celsius). A model of a multi-stage nanofiltration system such as Figure 9 Shown in the test flow for A, where the permeate from the filter is recycled into the storage / feed tank and filtered again to simulate a second or continuous filtration stage.

[0084] Liquid components are pre-filtered with a filter with a pore size of 1 micron. Put 30L of the pretreated biomass liquid component into the container and add about 1ml of antifoaming agent (KFO-119, purchased from Kabo Chemical...

Embodiment 3

[0086] In this example, the retentate samples from the dialysis and filtration in Example 2 were collected and fermented, so as to analyze the impact of the treatment process on fermentation anxiety. The collected samples contained varying concentrations of acetic acid and were fermented with 10 g / L (dry weight) of the genetically modified yeast strain Saccharomyces cerevisiae as described in US Patent 7,622,284 to Royal Nedalco B.V. 5 mg / L lactose (purchased from Lallemand Ethanol Technology, Milwaukee, WI), 62.5 g / L urea, and 1 g / L yeast extract were added to each fermenter, and the pH was adjusted to 5.5 with KOH. Such as Figure 16 Shown is the result after 24 hours of fermentation, ie, the graph of ethanol concentration (1602) versus fermentation time (1604). Similarly, Figure 17 Shown is a plot of ethanol production (1702) at the completion of the fermentation versus the initial acetic acid concentration (1704). The sample with an initial acetic acid concentration of...

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Abstract

Systems and methods for increasing fermentation efficiency of a lignocellulosic hydrolysate are disclosed. The system comprises a filter configurable to remove matter having a particle size of larger than about 25 to 100 microns from the liquid component, and at least one nanofilter configurable to remove acids from the liquid component. An apparatus is used to adjust the pH of the nanofiltered liquid component using a calcium hydroxide composition to a pH of about 5.5 to 6.0. The calcium hydroxide composition includes calcium hydroxide alone or in combination with either ammonium hydroxide and / or potassium hydroxide. The biomass comprises lignocellulosic material including at least one of corncob, corn plant husk, corn plant leaves, and corn plant stalks.

Description

[0001] cross reference [0002] This application claims priority to US Provisional Application No. 61 / 435,149, filed January 21, 2011, entitled "SYSTEMS AND METHODS FOR IMPROVING FERMENTATION," the disclosure of which is incorporated herein by reference. technical field [0003] Aspects disclosed herein relate to systems and methods for increasing the fermentation efficiency of lignocellulosic hydrolyzates using nanofiltration and a calcium hydroxide composition. Background technique [0004] Dilute acid treatment, as a preliminary step in lignocellulosic processing, is an effective way to hydrolyze a considerable portion of structural polysaccharides into monosaccharides and more digestible polysaccharide chains. During this process, the raw material is ground to a suitable fineness and undergoes a pretreatment process with acid and high temperature. This pretreatment process breaks down the raw material into a slurry. In order to fully separate the slurry compone...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/06B01D61/02C13B20/16
CPCB01D2317/022Y02E50/16C12M21/12C12M45/04B01D2311/04B01D61/027B01D2311/12C12M43/02C12P7/10Y02E50/17C13K1/02B01D61/022C07H1/08C13K1/06B01D2311/2649C13K13/002B01D2315/16B01D2311/18C12P2201/00C12M45/06Y02E50/10B01D61/0271
Inventor 尼拉康塔姆·V.·纳雷德拉纳斯史蒂文·T.·布莱
Owner POET RES INC
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