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Measuring method of absorption coefficients of total particulate matter and phytoplankton in the water

An absorption coefficient and phytoplankton technology, which is applied in the preparation of test samples and the measurement of color/spectral characteristics, can solve the problem of inaccurate determination of the absorption coefficient of phytoplankton in non-pigment particulate matter and the inability to extract phycobilin eukaryotic pigments well. And other issues

Inactive Publication Date: 2010-05-05
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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Problems solved by technology

[0004] The purpose of the invention is to solve the problem that phycobilin and some eukaryotic pigments cannot be well extracted when methanol is used to extract and remove phytoplankton when measuring the total particle absorption coefficient at home and abroad; when sodium hypochlorite is used to extract and remove phytoplankton, non-pigmentation The problem of inaccurate determination of particle absorption and phytoplankton absorption coefficient, and provide a method for measuring the total particle absorption coefficient and phytoplankton absorption coefficient in water

Method used

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  • Measuring method of absorption coefficients of total particulate matter and phytoplankton in the water
  • Measuring method of absorption coefficients of total particulate matter and phytoplankton in the water
  • Measuring method of absorption coefficients of total particulate matter and phytoplankton in the water

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specific Embodiment approach 1

[0029] Specific embodiment one: this embodiment method comprises the following steps:

[0030] Step 1. Fix the glass fiber filter membrane with a pore size of 0.7 μm and a diameter of 47 mm on the filter, put the water body to be tested into the filter, and obtain the filter membrane sample after filtering through the glass fiber membrane fixed on the filter. At the same time, use glass fiber membrane to filter pure water to make two blank samples.

[0031] Step 2, put the filter membrane sample and filter membrane blank sample obtained in step 1 into a spectrophotometer, and measure the absorbance OD of the filter membrane sample at different wavelengths i (λ), where λ represents the wavelength,

[0032] The wavelength λ ranges from 380nm to 800nm, with an interval of 1nm,

[0033] Step 3. According to the formula a(λ)=2.303×OD s (λ)×S / V calculation to obtain the total particle absorption coefficient a(λ),

[0034] Where: S is the effective area of ​​particles deposited o...

specific Embodiment approach 2

[0055] Embodiment 2. The difference between this embodiment and the embodiment is that the extraction time in step 4 is 40 minutes to 50 minutes, and other steps and parameters are the same as in the embodiment 1.

specific Embodiment approach 3

[0056] Embodiment 3. The difference between this embodiment and the embodiment is that the extraction time in step 4 is 45 minutes, and other steps and parameters are the same as in the embodiment 1.

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Abstract

A measuring method of absorption coefficients of total particulate matter and phytoplankton in the water relates to a measuring method of total particulate matter and components thereof. The invention aims to solve the problem that when measuring the absorption coefficient of total particulate matter at home and abroad, phycobilin and some pigments of eukaryotic cells can not be well extracted when methanol is used to extract for removing phytoplankton, and the absorption of non-pigment particulate matter and the inaccurate measurement of absorption coefficient are caused by using sodium hypochlorite to extract for removing phytoplankton. The method of the invention comprises the following steps: 1) preparing filtration film samples and a blank sample; 2) placing the samples in a spectrophotometer to measure the absorbance; 3) calculating the absorption coefficient of total particulate matter; 4) extracting filtration film samples with acetone to remove phytoplankton and retaining non-pigment particulate matter; 5) obtaining the absorbance of non-pigment particulate matter; 6) calculating the absorption coefficient of the non-pigment particulate matter; and 7) calculating the absorption coefficient of phytoplankton.

Description

technical field [0001] The invention relates to a method for measuring the absorption coefficient of total particle matter and the absorption coefficient of phytoplankton in a water body. The total particle absorption coefficient includes the absorption coefficient of phytoplankton and the absorption coefficient of non-pigment particles. Background technique [0002] The total particulate matter in the water body includes two parts, pigmented particles and non-pigmented particles. The pigmented particles refer to phytoplankton. The absorption coefficient of the total particulate matter in the water body is one of the most important parameters for water color remote sensing monitoring, directly affecting the transparency of the water body, water color and other optics. The characteristics determine the underwater distribution of sunlight, and ultimately affect the primary productivity of the water body. [0003] At present, the commonly used method for measuring the absorptio...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N1/34
Inventor 刘殿伟姜广甲宋开山王远东徐京萍张柏王宗明
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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