Method for preparing cigarette mainstream smoke total particulate matter sample for biological detection

A technology for cigarette mainstream smoke and biological detection, which is applied in the preparation of cigarette mainstream smoke total particulate matter samples and the field of cigarette mainstream smoke total particulate matter preparation, and can solve the problems of complicated process and unreported operation process, etc. Achieve the effect of simple operation steps

Active Publication Date: 2010-10-27
YUNNAN RES INST OF TOBACCO SCI
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Problems solved by technology

The processes of these existing methods for preparing total particulate matter in flue gas are all very complicated.
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  • Method for preparing cigarette mainstream smoke total particulate matter sample for biological detection

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Embodiment Construction

[0017] 1. Three preparation methods of total particulate matter in flue gas

[0018] The cigarettes were balanced in a constant temperature and humidity chamber for 48 hours, and cigarettes with consistent weight and draw resistance were selected. According to the national standard method, 20 cigarettes were smoked on the smoking machine, and the same sample was smoked three times, and the filters were marked as 1, 2, and 3. The first Cambridge filter is soaked in DMSO at 10mgTPM / ml (Cambridge filter is weighed before smoking),

[0019] 2. Comparative study on the mutagenicity of three different extraction methods in the detection of total particulate matter in flue gas

[0020] The TPM samples obtained by three different extraction methods were mixed with the Ames test plate, and the commercially purchased MOLTOX AMES ASSAY STDisc TA98 was used as the strain, and the TPM concentration was 25, 50, 75, 100, 125, 250, 500ug / dish. See Table 1 for the experimental data.

[0021...

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Abstract

The invention relates to a method for preparing a cigarette mainstream smoke total particulate matter sample for biological detection, which belongs to the technical field of safety assessment of tobaccos and cigarettes. The method for preparing the cigarette mainstream smoke total particulate matter sample for the biological detection is improved based on the prior art, wherein the improved points comprise the following steps of: a, directly soaking a Cambridge filter which catches cigarette mainstream smoke total particulate matters in an organic solvent dimethyl sulfoxide, performing ultrasonic extraction for 20 minutes, filtering a mixture by using filter paper, subpackaging a filtrate to a 2 ml freezing tube, and reserving the freezing tube in an ultra-low temperature freezer at the temperature of 70 DEG C below zero for later use; and b, adding the organic solvent dimethyl sulfoxide at the speed of 10 mg TPM/ml at one time without setting a constant volume. The method for preparing the cigarette mainstream smoke total particulate matter sample for the biological detection has the advantages of simple and convenient operation steps, and capacity of truthfully reflecting the mutagenicity of the cigarette mainstream smoke total particulate matters.

Description

Technical field: [0001] The invention relates to a method for preparing a sample of total particulate matter in mainstream cigarette smoke for biological detection, in particular to a method for preparing total particulate matter in mainstream smoke of cigarettes (also called smoke condensate sample), which belongs to tobacco and cigarettes The technical field of safety evaluation. Background technique: [0002] The preparation of the total particulate matter sample of cigarette smoke (also called smoke condensate sample) is an important link in the detection of biological activity of mainstream cigarette smoke. Biological activity detection such as reverse mutation test, cytotoxicity test and micronucleus test. There are many methods for preparing smoke samples for biological activity detection reported in the literature. Domestic literature [1] reported that ethyl acetate and cell culture fluid were used to absorb and capture all the flue gas condensate. The method of d...

Claims

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Application Information

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IPC IPC(8): C12Q1/24
Inventor 李雪梅黄海涛唐萍徐济仓耿永勤缪恩铭
Owner YUNNAN RES INST OF TOBACCO SCI
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