Method for preparing cigarette mainstream smoke total particulate matter sample for biological detection

Abstract

The invention relates to a method for preparing a cigarette mainstream smoke total particulate matter sample for biological detection, which belongs to the technical field of safety assessment of tobaccos and cigarettes. The method for preparing the cigarette mainstream smoke total particulate matter sample for the biological detection is improved based on the prior art, wherein the improved points comprise the following steps of: a, directly soaking a Cambridge filter which catches cigarette mainstream smoke total particulate matters in an organic solvent dimethyl sulfoxide, performing ultrasonic extraction for 20 minutes, filtering a mixture by using filter paper, subpackaging a filtrate to a 2 ml freezing tube, and reserving the freezing tube in an ultra-low temperature freezer at the temperature of 70 DEG C below zero for later use; and b, adding the organic solvent dimethyl sulfoxide at the speed of 10 mg TPM / ml at one time without setting a constant volume. The method for preparing the cigarette mainstream smoke total particulate matter sample for the biological detection has the advantages of simple and convenient operation steps, and capacity of truthfully reflecting the mutagenicity of the cigarette mainstream smoke total particulate matters.

Description

Technical field: [0001] The invention relates to a method for preparing a sample of total particulate matter in mainstream cigarette smoke for biological detection, in particular to a method for preparing total particulate matter in mainstream smoke of cigarettes (also called smoke condensate sample), which belongs to tobacco and cigarettes Technical field of safety evaluation. Background technique: [0002] The preparation of the total particulate matter sample of cigarette smoke (also called smoke condensate sample) is an important link in the detection of biological activity of mainstream cigarette smoke. Biological activity detection such as reverse mutation test, cytotoxicity test and micronucleus test. There are many methods for preparing smoke samples for biological activity detection reported in the literature. Domestic literature [1] reported that ethyl acetate and cell culture fluid were used to absorb and capture all the flue gas condensate. The method of domes...

AI Technical Summary

Problems solved by technology

The processes of these existing methods for preparing total particulate matter in flue gas are all very complicated.

Foreign literature [5-6] used dimethyl sulfoxide for the preparation of flue gas condensate, but did not report the specific operation process

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