Method for detecting influence of total particulate matters in cigarette smoke on reactive oxygen secretion of cells

Abstract

The invention discloses a method for detecting the influence of total particulate matters in cigarette smoke on reactive oxygen secretion of cells. The method comprises the following steps: (1) pretreatment of to-be-detected total particulate matters in cigarette smoke; (2) preparation of monoplast suspension of human lung fibroblasts; (3) calculation of the cell concentration of the monoplast suspension of human lung fibroblasts; (4) cell inoculation of the monoplast suspension of human lung fibroblasts; (5) grouping of the to-be-detected total particulate matters in cigarette smoke; (6) setting of the detection dosages of the to-be-detected total particulate matters; (7) preparation of cultivated to-be-detected total particulate matters; (8) incubation of the to-be-detected total particulate matters; (9) addition of positive controls; (10) probe loading; (11) sample determination; and (12) calculation of reactive oxygen species. Through effective treatment of samples, optimization of the detection dosages and correct selection of target cells, the method is capable of accurately and effectively detecting the influence of the total particulate matters in cigarette smoke on reactive oxygen species in cells.

Description

technical field [0001] The invention belongs to the technical field of biological effect evaluation of tobacco products, and in particular relates to a method for detecting the influence of total particulate matter in cigarette smoke on the secretion of active oxygen in cells. Background technique [0002] As people pay more and more attention to health, higher requirements are put forward for tobacco products, not only to have a better taste, but also to reduce the adverse feelings of the human body as much as possible. Cigarette product R&D personnel combine different components according to different proportions in the initial stage of e-liquid product formula design to form primary formulas with different styles, and then continuously screen and adjust the formulas in combination with chemical evaluation and sensory evaluation to form the final product formula. However, there are a large number of primary formulations, and sensory evaluation of all of them is time-consum...

AI Technical Summary

Problems solved by technology

However, there are a large number of primary formulas, and sensory evaluation of all of them is time-consuming and labor-intensive, and the sensory evaluation focuses on screening the style and taste of the product. How to use biological test indicators to screen the primary formula of the product to obtain the effect of reducing the adverse feelings of the human body? The product formula is still in the exploratory stage, and there is no uniform standard method

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