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Method for measuring bioamine in main stream smoke of cigarettes

A technology for cigarette mainstream smoke and determination methods, which is applied to measuring devices, instruments, scientific instruments, etc., can solve problems such as complex operations, and achieve the effects of high sensitivity, low detection limit, good repeatability and recovery rate

Active Publication Date: 2017-02-22
CHINA TOBACCO HENAN IND
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), thin-layer chromatography (TLC) and gas chromatography (GC) are generally used for the detection of biogenic amines. The above-mentioned detection methods all need to derivatize the sample, and the operation is relatively complicated.

Method used

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  • Method for measuring bioamine in main stream smoke of cigarettes
  • Method for measuring bioamine in main stream smoke of cigarettes
  • Method for measuring bioamine in main stream smoke of cigarettes

Examples

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Embodiment 1

[0032] A method for assaying biogenic amines in tobacco, comprising the following steps,

[0033] (1) Prepare a mixed series of standard working solutions: prepare a mixed series of standard working solutions with a concentration gradient and containing an internal standard;

[0034] (2) Sample pretreatment: place the cigarette sample in an environment with a relative humidity of 60% at 22°C for 48 hours, and then select cigarettes whose average weight is within the range of ±0.02g and average draw resistance within the range of ±49Pa / cigarette As a test cigarette. A rotary smoking machine is used to take a puff every 60 seconds, with a suction volume of 35ml per puff, and a puff duration of 2s. Use a Cambridge filter with a diameter of 92mm to capture the total particulate matter of mainstream cigarette smoke. Each filter piece collects the total particulate matter of 20 cigarettes. Take one filter piece that collects the total particulate matter of 20 cigarettes and place i...

Embodiment 3

[0060] Get cigarette sample 1-5#, carry out sample analysis according to the assay method of the present invention, sample analysis result is shown in Table 5.

[0061] Content of biogenic amines in table 5 tobacco leaf samples

[0062]

[0063]

[0064] Note: "-" means not detected in the sample.

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Abstract

The invention belongs to the method for measuring bioamine in main stream smoke of cigarettes. The method comprises the following steps: (1) preparation of a mixed series standard working : preparing the mixed series standard working solution with concentration gradient and containing an interior standard; (2) pretreatment of a sample: adding a cambridge filter gathered with total particulate matters of the main stream smoke of the cigarettes into 2% of a formic acid aqueous solution, and ultrasonically extracting for 30 minutes; (3) purification with solid-phase extraction column: enabling extract liquor obtained in the step (2) to flow through a mixed type cation exchange solid-phase extraction column, after eluting, blow-drying eluant under nitrogen, and redissolving with 2 mL of an acetonitrile aqueous solution to obtain sample purifying liquid; (4) carrying out HPLC-MS / MS measurement on the mixed series standard working solution containing the internal standard obtained in the step (2) and the sample purifying liquid obtained in the step (3) under the same conditions; and (5) result calculation: quantifying by using an internal standard method according to the ratio of the peak area of a targeted analyzed object to the peak area of the internal standard.

Description

technical field [0001] The invention relates to the technical field of detection of components in cigarette smoke, in particular to a method for measuring biogenic amines in mainstream smoke of cigarettes. Background technique [0002] Biogenic amine is a general term for a class of biologically active nitrogen-containing low-molecular-weight basic organic compounds. They are precursors for the synthesis of hormones, nucleotides, and proteins by organisms themselves. They can be regarded as 1-3 hydrogens in ammonia molecules Low-molecular-weight alkaloids formed after atoms are substituted by alkyl or aryl groups. Biogenic amines can be divided into three categories according to their structures: aliphatic (putrescine, cadaverine, spermine, spermidine, etc.), aromatic amines (tyramine, β-phenylethylamine, etc.) and heterocyclic amines (histamine, tryptamine, etc.). [0003] Biogenic amines are mainly formed by the decarboxylation of amino acids, and a small part is formed ...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88
Inventor 付瑜锋田海英胡少东于建春王宏伟段鹍李耀光王宝林郝辉王鹏飞
Owner CHINA TOBACCO HENAN IND
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