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Method for detecting specific N-nitrosamines in tobacco in total particulate matters of mainstream smoke

A technology for mainstream smoke and particulate matter, which is applied in the field of tobacco analysis, can solve the problems of inability to detect and maximize the performance of the instrument, and achieve the effect of high accuracy and high detection sensitivity

Active Publication Date: 2014-04-16
JILIN TOBACCO IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the analysis conditions recommended in GB / T23228-2008, in the face of the sensitivity of the TEA800 thermal energy analyzer that is commonly used now, and the current situation that the content of TSNAs in Chinese cigarettes is relatively small, cannot give full play to the maximum performance of the instrument during the detection process, and even cause failure Detected situation

Method used

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  • Method for detecting specific N-nitrosamines in tobacco in total particulate matters of mainstream smoke
  • Method for detecting specific N-nitrosamines in tobacco in total particulate matters of mainstream smoke
  • Method for detecting specific N-nitrosamines in tobacco in total particulate matters of mainstream smoke

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0097] Accurately weigh 10 mg of N-pentyl-(3-methylpyridyl) nitrosamine, add about 50 mL of dichloromethane to completely dissolve it, transfer it to a 100 mL brown volumetric flask, and dilute to the mark with dichloromethane to obtain internal standard stock solution;

[0098] Accurately pipette 2mL internal standard stock solution into a 50mL brown volumetric flask, and dilute to the mark with dichloromethane to obtain a primary internal standard solution.

[0099] Accurately pipette 10mL of the primary internal standard solution into a 100mL brown volumetric flask, and dilute to the mark with dichloromethane to obtain the secondary internal standard solution.

[0100] Accurately weigh 10 mg of NNN, NAT, NAB and NNK respectively, add about 5 mL of dichloromethane to dissolve completely, transfer to a 10 mL brown volumetric flask, and dilute to the mark with dichloromethane to obtain a standard stock solution.

[0101] Accurately pipette 1mL standard stock solution into a 1...

Embodiment 2

[0116] The technical scheme described in Example 1 was used to detect the standard solutions with a series of concentrations. The difference was that in this example, the sample volume was 5 μL, the temperature of the injection port was 255° C., and the flow rate was 7 mL / min.

[0117] Detect and obtain the chromatogram of series concentration standard solution, the result is as follows image 3 as shown, image 3 It is the chromatogram of the standard solution obtained in Example 2 of the present invention. The present invention will image 3 The obtained chromatograms were integrated, and the results are shown in Table 2. Table 2 is an integral table of the standard solution chromatograms obtained in Example 2 of the present invention.

[0118] The integral table of the standard solution chromatogram that the embodiment of the present invention 2 of table 2 obtains

[0119]

[0120]

[0121] The present invention obtains the peak area according to integration and th...

Embodiment 3

[0123] The technical scheme described in Example 1 is used to detect the standard solutions of series concentrations. The difference is that in this example, the injection volume is 5 μL, the pulse is splitless, the temperature of the injection port is 250 ° C, and the flow rate is 8 mL. / min.

[0124] Detect and obtain the chromatogram of series concentration standard solution, the result is as follows Figure 5 as shown, Figure 5 It is the chromatogram of the standard solution that the embodiment 3 of the present invention obtains. The present invention will Figure 5 The obtained chromatograms were integrated, and the results are shown in Table 3, which is an integral table of the standard solution chromatograms obtained in Example 3 of the present invention.

[0125] The integral table of the standard solution chromatogram that the embodiment of the present invention 3 of table 3 obtains

[0126]

[0127] The present invention obtains the peak area according to int...

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Abstract

The invention provides a method for detecting specific N-nitrosamines in tobacco in total particulate matters of mainstream smoke. The method comprises the following steps: providing a to-be-detected solution containing mainstream smoke particulate matters and an internal standard substance, wherein the internal standard substance is N-amyl-(3-methyl pyridine) nitrosamines; carrying out gas chromatography-thermal energy analysis on the to-be-detected solution to obtain a to-be-detected solution chromatographic curve, wherein the sample feeding mode of the gas chromatography-thermal energy analysis is pulse without shunting; calculating the content of specific N-nitrosamines in tobacco in the to-be-detected solution according to the to-be-detected solution chromatographic curve and a preset standard curve; and calculating the content of N-nitrosamines in tobacco in total particulate matters of mainstream smoke according to the content of specific N-nitrosamines in tobacco in the to-be-detected solution. Therefore, the method provided by the invention has high sensitivity and accuracy on the detection of the specific N-nitrosamines in tobacco in total particulate matters of mainstream smoke.

Description

technical field [0001] The invention relates to the technical field of tobacco analysis, in particular to a method for detecting tobacco-specific N-nitrosamines in the total particulate matter of mainstream smoke. Background technique [0002] Tobacco-specific N-nitrosamines (TSNAs) include N-nitrosonornicotine (NNN), 4-(methylnitrosamine)-1-(3-pyridyl)-1-butanone (NNK ), 4-(N-methyl-N-nitrosoamino)-4-(3-pyridyl)butyraldehyde (NNA), N-nitrosoneonicotinoid (NAB) and N-nitrosoneonicotinoid Nicotine (NAT); the first three are derived from nicotine and nornicotine, and the latter two are formed from anatabine and anatabine, respectively. TSNA is the most important class of nitrosamines in tobacco. Studies have shown that such substances have strong carcinogenicity. Therefore, it is very important to establish an accurate method for the determination of TSNA in tobacco leaves, cigarettes, and smoke. ways to reduce its content in order to improve the safety of cigarette smoking....

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 史中华崔龙吉周萍郭晓玉李锋姜滢
Owner JILIN TOBACCO IND
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