Hsa-miR-489 detection kit based on AllGlo probe fluorescent quantitative PCR (Polymerase Chain Reaction) and detection method thereof

A technology of hsa-mir-489 and cel-mir-39, applied in the field of MicroRNA, can solve the problems of unfavorable promotion and expensive synthesis, and achieve the effects of low background signal, fast and accurate detection, and simple synthesis procedure
CN103757121AInactive Publication Date: 2014-04-30ZHONGSHAN HOSPITAL XIAMEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHONGSHAN HOSPITAL XIAMEN UNIV
Publication Date
2014-04-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

Ahsa-miR-489detection kit based on AllGlo probe fluorescent quantitative PCR (Polymerase Chain Reaction) and a detection method of the hsa-miR-489detection kit relate to MicroRNA. The detection kit is provided with a kit body, a clapboard, anexogenous reference bottle, a stem-loop reverse transcription reagent bottle, and a real-time fluorescent quantitative PCR reagent bottle. The miRNA in a sample is extracted, and if the sample is a serum / plasma sample or other liquid samples, then 5 mu L exogenous reference cel-miR-39 with the concentration of 5nmol, provided by the reagent bottle, is added after the sample is fully split, and the mixture is oscillated in vortex for 15s; but if the sample is a cell or tissue sample, then exogenous reference cel-miR-39 is not added; a stem-loop reverse transcription reagent provided by the reagent bottle is used to reversely transcribemiRNA into cDNA; a real-time fluorescent quantitative PCR reagent provided by the reagent bottle is used to conduct real-time PCR amplification; various data provided by an analytical instrument are integrated together, and reasonable thresholds and reference lines are set for result analysis.
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Description

technical field

[0001] The invention relates to MicroRNA, in particular to a hsa-miR-489 detection kit and detection method based on AllGlo probe fluorescence quantitative PCR. Background technique

[0002] MicroRNA (miRNA) is a kind of non-coding RNA molecule of about 22 nucleotides widely present in eukaryotic cells, which participates in many physiological and pathological processes in organisms, by regulating gene expression, in cell proliferation, apoptosis , growth and development, cell differentiation, metabolism and other processes play an important role. Specifically, miRNA forms the initial primary transcript pri-miRNA to pre-miRNA in the nucleus, and then transports out of the nucleus to form mature miRNA through cleavage. Form RISC (RNA-induced silencing complex) with Ago protein, partially inhibit or degrade target mRNA sequence, and participate in gene expression regulation (Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function [J]. Cell, 2004, 11...

Claims

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