The invention relates to the technical field of medical instruments, in particular to a blood purification device. The device comprises a group of filtering assemblies, a positioning assembly, a dialysis tube, an upper tube cover and a lower tube cover, each filtering assembly comprises an outer pipe and an inner pipe connected into the outer pipe in a penetrating mode, the positioning assembly comprises a first circular plate and a second circular plate, a liquid inlet tube is arranged on the tube body at one end of the dialysis tube close to the lower tube cover, grooves are formed in the surfaces of the upper tube cover and the lower tube cover, a cavity between the first circular plate and the second circular plate in each groove is a blood chamber, a cavity between the second circularplate and the bottom of each groove in each groove is a liquid chamber, a liquid outlet pipe and a blood outlet pipe are arranged on the lower tube cover, a blood inlet pipe is arranged on the uppertube cover, and a group of flow guide grooves are formed in the upper tube cover and communicate the interior of the dialysis tube with the liquid chamber of the upper tube cover. According to the device, the membrane rupture phenomenon can be effectively avoided, and meanwhile the contact area of blood and dialyzate in a dialyzer can be effectively increased, and the affinity to the body of a patient can be effectively improved.