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Anti-inflammatory active pharmaceutical composition based on radix bupleuri component and application of anti-inflammatory active medicinal composition

A technology of active drugs and compositions, applied in the field of anti-inflammatory active drug compositions based on components of Bupleurum bupleurum and its preparation, can solve problems such as endangering human health, uncontrollable inflammatory reactions, and abnormal immune regulation capabilities

Active Publication Date: 2019-08-16
WENZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, under certain pathological conditions, due to abnormal immune regulation, a series of uncontrollable inflammatory reactions will be caused, which will seriously endanger human health.

Method used

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  • Anti-inflammatory active pharmaceutical composition based on radix bupleuri component and application of anti-inflammatory active medicinal composition
  • Anti-inflammatory active pharmaceutical composition based on radix bupleuri component and application of anti-inflammatory active medicinal composition
  • Anti-inflammatory active pharmaceutical composition based on radix bupleuri component and application of anti-inflammatory active medicinal composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Preparation of Bei Bupleurum Oligosaccharide Fragment (BCO)

[0023] Take 5 kg of Bupleurum bupleurum medicinal material, bake at 80°C to constant weight, crush and sieve the dried Bupleurum bupleuri (40 mesh), add 8 times the amount of 80% ethanol, adjust pH=8, reflux extraction at 80°C for 3 times, each Combine the ethanol extracts for the preparation of total saponins, extract the residue 4 times in hot water at 80°C for 2 hours each time, filter with suction, combine the filtrates, concentrate the filtrates under reduced pressure at 60°C, and add 5 times the volume Precipitate in absolute ethanol, let stand at low temperature overnight, centrifuge, add distilled water to dissolve the precipitate, repeat the step of ethanol precipitation 3 times, dissolve with distilled water, after repeated freeze-thaw and centrifuge to remove insoluble matter, add streptomyces protease to digest the supernatant In 24 hours, deproteinized five times by Sevag method, conce...

Embodiment 2

[0024] Embodiment 2: the preparation of northern Bupleurum saponins (BCS)

[0025] First filter the ethanol extract to remove insoluble matter, recover ethanol by rotary evaporation, concentrate the volume, extract 3 times with 5 times the amount of water-saturated n-butanol, recover n-butanol, and obtain the saponin extract, and use 1 column volume per hour Load the sample to the AB-8 macroporous resin column at a flow rate of 5 times the column volume, and then use 5 times the column volume of distilled water to elute at a flow rate of 2 times the column volume per hour, and then use 5 times the column volume of 70% ethanol to elute at a flow rate of 2 times the column volume per hour. Flow rate elution, collecting 70% ethanol eluate, recovering and concentrating, and drying in vacuum to obtain a refined sample of total saponins of Bupleurum bupleuri.

Embodiment 3

[0026] Example 3: Inhibitory effect of Bei Bupleurum traditional Chinese medicine composition on acute peritonitis in mice

[0027] Male mice were injected intraperitoneally with 2 mL of 3% sodium thioglycolate medium or sterile saline. After 10 minutes, inject Bupleurum oligosaccharide fragments, total saponins, and compositions of different ratios in the tail vein of mice (oligosaccharide fragments: total saponins mass percentages were 10:90; 25:75; 50:50; 75:25; 90:10). After 2 hours, the mice were sacrificed, lavaged with normal saline, and the peritoneal fluid was collected. After centrifugation, cells were fixed, smeared and stained, and the number of white blood cells per milliliter of lavage fluid was counted, and the inhibition rate was calculated. The results showed that compared with the control group, after the intervention of Bupleurum oligosaccharide fragments, total saponins and compositions with different ratios, they exhibited different degrees of inhibition o...

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Abstract

The invention belongs to the field of traditional Chinese medicines, and particularly relates to an anti-inflammatory active pharmaceutical composition based on radix bupleuri components and a preparation method of the anti-inflammatory active pharmaceutical composition. The components of the composition are from the radix bupleuri, and particularly, the composition consists of the following components in percentages by mass: 10-50% of a radix bupleuri oligosaccharide fragment and 50-90% of radix bupleuri total saponins. According to pharmacological and activity experimental results, the composition can effectively inhibit exudation of white blood cells in the peritoneal fluid of an acute peritonitis mouse model, and shows good anti-inflammatory activity; and the acting mechanism of the composition is closely related to antagonizing leukocyte adhesion mediated by vascular endothelial P-selectin. The results show that the radix bupleuri composition can be used for preparing novel effective medicines for treating acute inflammation, and has wide application prospect.

Description

technical field [0001] The present invention relates to a pharmaceutical composition, in particular to a pharmaceutical composition with anti-inflammatory effect, in particular to an active anti-inflammatory pharmaceutical composition based on Bupleurum bupleuri components and a preparation method thereof. Background technique [0002] Bupleurum chinense is one of the traditional and commonly used bulk Chinese herbal medicines in China. The Pharmacopoeia of the People's Republic of China (2015 edition) contains the perennial herb Bupleurum chinense DC. and Bupleurum chinense DC. and Bupleurum chinense DC. Bupleurum scorzonerifolium Willd.) is the medicinal part of Bupleurum scorzonerifolium Willd.), and Northern Bupleurum is the mainstream variety of Bupleurum scorzonerifolium Willd., which has been used in my country for more than 2,000 years. Bupleurum pungent, bitter, slightly cold in nature, returns to the liver, gallbladder, and lung meridians, and has the effects of rec...

Claims

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Application Information

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IPC IPC(8): A61K36/233A61K31/702A61P29/00
CPCA61K31/702A61K36/233A61K2236/331A61K2236/333A61K2236/39A61K2236/53A61K2236/55A61P29/00A61K2300/00
Inventor 佟海滨吴明江吴思雅
Owner WENZHOU UNIVERSITY
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