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Method for culturing, planting and mycorrhizal production of dendrobium officinale kimura et migo

A Dendrobium officinale and production method technology, applied in the direction of botany equipment and methods, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of low content of active ingredients, low cultivation survival rate, slow cultivation growth, etc., to achieve The effect of enhanced stress resistance, simple cultivation method, and increased fresh weight and dry weight

Inactive Publication Date: 2011-08-17
梁经军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method can solve the problems of low reproduction and cultivation survival rate of Dendrobium candidum, slow cultivation growth, long cycle, low content of active ingredients, poor drug efficacy, etc.

Method used

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  • Method for culturing, planting and mycorrhizal production of dendrobium officinale kimura et migo
  • Method for culturing, planting and mycorrhizal production of dendrobium officinale kimura et migo

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The operation steps of LP2 group are as follows:

[0028] 1. Culture of mycorrhizal fungi

[0029] In this example, the mycorrhizal fungus LP2 strain was used to activate the slant test tube strains preserved at low temperature, and then placed on a PDA plate, cultured at a constant temperature of 25°C for 12 days, and punched into bacterial slices (Φ=8 mm) at the edge of the colony, and Access liquid medium in small pieces. The mycorrhizal fungus LP2 belongs to the genus Fusarium ( Fusarium sp. ), the bacterial strain is currently preserved in the China Medicinal Microorganism Culture Collection Management Center, and the preservation number is CPCC810233.

[0030] The mycorrhizal fungus uses a liquid medium, and the preparation of the medium: prepared with water, glucose 20 g / L, KH 2PO 4 20 g / L, MgSO 4 1.5 g / L, vitamin B 1 10 mg / L, natural product: wheat bran 30 g / L (boiled juice), medium pH=6.0. After autoclaving, insert the above-mentioned fungi into each...

Embodiment 2

[0049] The present embodiment basic operation condition is the same as embodiment 1, and difference is:

[0050] 1. The mycorrhizal fungi used in this embodiment are mycorrhizal fungi LP2 and mycorrhizal fungi LP3. The mycorrhizal fungus LP2 belongs to the genus Fusarium ( Fusarium sp. ), the mycorrhizal fungus LP3 belongs to the genus Verticillium ( Verticillium sp. ). The two strains are currently preserved in the China Medicinal Microorganism Culture Collection and Management Center, and the preservation numbers are CPCC810233 and CPCC421139 respectively.

[0051] 2. The fungal strains are cultured in solid culture. The solid culture medium for mycorrhizal fungi is composed of: cottonseed hulls and crushed corn cobs are mixed at a weight ratio of 1:1, and then water is added. The amount of water added is 170 wt% of the weight of the raw materials. After autoclaving, the above-mentioned fungi were inoculated respectively, and 2 bacterial slices (Φ=8 mm) were inoculated ...

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Abstract

The invention relates to a method for culturing, planting and mycorrhizal production of dendrobium officinale kimura et migo, comprising the following steps of: (1) culturing mycorrhizal fungi by utilizing a solid or liquid culture medium, wherein the mycorrhizal fungi are mycorrhizal fungi LP2 deuteromycotina Fusariumsp, mycorrhizal fungi LP3, or mixed strain deuteromycotina Verticilliumsp of the mycorrhizal fungi LP2 deuteromycotina Fusariumsp and the mycorrhizal fungi LP3; (2) culturing tissue culture seedlings of the dendrobium officinale kimura et migo; (3) preparing a nutrient solution; (4) carrying out water culture and training the tissue culture seedlings; and (5) carrying out planting and mycorrhizalculturing of the dendrobium officinale kimura et migo. The method for culturing, planting and mycorrhizal production of the dendrobium officinale kimura et migo, provided by the invention, can promote growth of dendrobium officinale kimura et migo seedlings obviously; and compared with a contrast plant, the dendrobium officinale kimura et migo cultured by inoculating pathogen has increased rooting numbers, high survival rate, longer plant length, better growth vigor, obviously expanded internodes, obviously big stems, improved fresh weight and dry weight and obviously enhanced stress resistance of a pathogen-inoculated plant.

Description

technical field [0001] The invention relates to a mycorrhizalization method for the cultivation and planting of dendrobium officinale. Specifically relate to a kind of application method of mycorrhizal fungus in the cultivation of Dendrobium candidum, it comprises the cultivation of the mycorrhizal fungus used in the present invention, the development of Dendrobium candidum from protocorm to seedling and its subculture, mycorrhizal fungus in the cultivation of Dendrobium candidum The application method and the growth-promoting effect of Dendrobium officinale and the effects on metabolic activity, stress resistance, and content of active ingredients. Background technique [0002] Dendrobium officinale ( Dendrobium officinale Kimura et Migo ) is a rare and endangered high-value-added medicinal material, and its health care and therapeutic effects are exact. are recorded in. In the first year of Tang Kaiyuan, the Taoist classic "Tao Zang" included nine Chinese immortal grass...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N1/14C12R1/645C12R1/77
Inventor 赵兴兵张苏锋梁经军高正龙吴维佳黄生云崔晓娟李杰
Owner 梁经军
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