Extraction method for phorbol

An extraction method and phorbol technology, applied in the field of phorbol extraction, can solve the problems of low purification purity, complex extraction process, large solvent and the like, and achieve the effects of simple extraction process, reduced solvent consumption and improved yield

Inactive Publication Date: 2018-11-16
湖州展舒生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In order to solve the problems of using traditional silica gel column chromatography in the existing phorbol alcohol extraction process, which requires a large amount of solvent consumption, and the extraction process is complicated and the purification purity is not high, the present invention provides a simple and easy phorbol Alcohol extraction method; including the following:

Method used

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Examples

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Effect test

Embodiment

[0021] The present embodiment provides a kind of extraction method of phorbol alcohol, comprises the steps:

[0022] (1) Get 5 kilograms of maple seeds and carry out physical pulverization;

[0023] (2) Reflux with 8 liters of 95% ethanol solution for 2 hours, extract twice, then concentrate to dryness;

[0024] (3) The product concentrated to dryness in step (2) is extracted with 3 liters of ethyl acetate and then concentrated to dryness;

[0025] (4) Take the concentrated product in step (3), add 2 liters of 5% sodium hydroxide solution, and stir until TLC shows that the alkaline hydrolysis is complete;

[0026] (5) Recrystallize the crystalline product obtained in step (4) with absolute ethanol; white needle crystals are obtained. The purity of the phorbol obtained by this method reaches 98.75%; moreover, the extraction process of the present invention is simple, and 2% sodium hydroxide is used for alkaline hydrolysis, which greatly improves the yield of extraction, and r...

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Abstract

The invention provides an extraction method for phorbol to overcome the problems that conventional silica gel column chromatography is employed for phorbol extraction in the prior art and consumes a large amount of solvents and conventional extraction processes are complicated and have low purification efficiency. The extraction method for phorbol in the invention comprises the following steps: (1) weighing the seeds of Jatropha carcass and carrying out physical pulverization; (2) carrying out refluxing with an ethanol solution, then carrying out extraction, and then concentrating the obtainedextract until the extract is dry; (3) extracting a product concentrated to dryness in the step (2) with ethyl acetate and then carrying out concentration to dryness; (4) weighing a concentrated product obtained in the step (3), adding a sodium hydroxide solution and carrying out stirring until TLC shows that alkaline hydrolysis is completed; and (5) subjecting a crystallization product obtained in the step (4) to recrystallization with absolute ethanol so as to obtain a white needle crystal.

Description

technical field [0001] The invention relates to plant extracts, in particular to a method for extracting phorbol alcohol. Background technique [0002] Phorbol alcohol, English name: phorbol; due to its very complex structure, artificial synthesis is still very difficult, currently it is mainly extracted from croton oil, the existing extraction method uses traditional silica gel column chromatography, which needs to consume a large amount of organic solvents, And the efficiency is extremely low, and the purity of the obtained phorbol is not high. The phorbol alcohol extraction method provided by the invention is simple, consumes less solvent, and has high product purity. Contents of the invention [0003] In order to solve the problems of using traditional silica gel column chromatography in the existing phorbol alcohol extraction process, which requires a large amount of solvent consumption, and the extraction process is complicated and the purification purity is not hig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C49/727C07C45/79C07C45/81
CPCC07C45/79C07C45/81C07C2603/40C07C49/727
Inventor 吴建华
Owner 湖州展舒生物科技有限公司
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