Application of phenformin as skin anti-inflammatory preparation

A technology of phenformin and skin inflammation, which is applied in the field of phenformin as an anti-inflammatory skin preparation, can solve problems such as clinical treatment difficulties, and achieve the effect of reducing the inflammatory response of human skin epithelial cells

Active Publication Date: 2017-11-03
SHANDONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Dermatitis refers to a general term for skin inflammatory diseases caused by various internal and external infections or non-infectious factors. It is not an independent disease. Its etiology and clinical manifestations are complex and diverse, and it recurs repeatedly, making clinical treatment difficult.

Method used

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  • Application of phenformin as skin anti-inflammatory preparation
  • Application of phenformin as skin anti-inflammatory preparation
  • Application of phenformin as skin anti-inflammatory preparation

Examples

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Embodiment

[0064] Materials and Methods

[0065] 1) Isolation and culture of human primary skin cells:

[0066] Pretreat the tissue in 70% ethanol for 30 seconds, put it into PBS containing 2% penicillin and streptomycin for sterilization, then mince the tissue into a homogenate and transfer it to a 50ml centrifuge tube containing enzyme digestion solution at 37° Shake in a water bath for 1 hour, then add 0.25% trypsin to digest for 30 minutes; after digestion, add the same volume of DMED medium containing 10% fetal bovine serum, repeatedly blow and filter, centrifuge at 1000xg for 5 minutes, discard the supernatant, repeat once, the cells The pellet was resuspended in epidermis medium and cultured by adding Rock inhibitor Y27632.

[0067] 2) Treatment of epidermal cells with phenformin:

[0068] Take the cultured epidermal cells (P3) and inoculate them in a six-well plate, use 2ml medium for each well of 300,000 cells, replace the medium 24 hours after inoculation, and then use differ...

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Abstract

The invention discloses application of phenformin as a skin anti-inflammatory preparation. First discovery finds that the phenformin can be used for obviously inhibiting skin epidermic cells from secreting various inflammatory cell factors including 6 / 8(IL-6, IL-8) and tumor necrosis factors (TNF alpha), and similarly inflammatory response of human skin epithelial cells induced by frequently-used 12-oxy-tetradecanoyl phorbol-13-acetate (TPA) and poly-poly(C) polyinosinic-polycytidylic acid (poly I:C) (immunologic stimulant), so that the phenformin can be used for preventing and curing related diseases of skin inflammation. Therefore, novel application is developed for the phenformin, and new prevention and treatment means is provided for related diseases of skin inflammation.

Description

technical field [0001] The invention belongs to the field of medicine and pharmacy, and in particular relates to the use of phenformin as a skin anti-inflammatory preparation. Background technique [0002] Dermatitis refers to a general term for skin inflammatory diseases caused by various internal and external infections or non-infectious factors. It is not an independent disease. Its etiology and clinical manifestations are complex and diverse, and it recurs repeatedly, making clinical treatment difficult. According to modern medical research, the etiology of dermatitis diseases is very complicated, which may be related to the following factors: including internal factors such as chronic infection lesions (such as chronic cholecystitis, tonsillitis, intestinal parasitic diseases, etc.), endocrine and metabolic changes (such as menstrual disorders, pregnancy, etc.), blood circulation disorders (such as calf varicose veins, etc.), neuropsychiatric factors, genetic factors; e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/155A61P29/00A61P17/00A61P17/04A61P17/10A61P17/02
CPCA61K31/155
Inventor 吴训伟李丁洋周倩郭泾
Owner SHANDONG UNIV
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