Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Use of phenformin in the preparation of anti-inflammatory preparations for skin

A skin inflammation and phenformin technology, which is applied in the field of phenformin in the preparation of anti-skin inflammation preparations, can solve problems such as difficulties in clinical treatment, achieve the effects of preventing and treating skin inflammation-related diseases and reducing the inflammatory response of human skin epithelial cells

Active Publication Date: 2021-03-16
SHANDONG UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Dermatitis refers to a general term for skin inflammatory diseases caused by various internal and external infections or non-infectious factors. It is not an independent disease. Its etiology and clinical manifestations are complex and diverse, and it recurs repeatedly, making clinical treatment difficult.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Use of phenformin in the preparation of anti-inflammatory preparations for skin
  • Use of phenformin in the preparation of anti-inflammatory preparations for skin
  • Use of phenformin in the preparation of anti-inflammatory preparations for skin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0063] Materials and Methods

[0064] 1) Isolation and culture of human primary skin cells:

[0065] Pretreat the tissue in 70% ethanol for 30 seconds, put it into PBS containing 2% penicillin and streptomycin for sterilization, then mince the tissue into a homogenate and transfer it to a 50ml centrifuge tube containing enzyme digestion solution at 37° Shake in a water bath for 1 hour, then add 0.25% trypsin to digest for 30 minutes; after digestion, add the same volume of DMED medium containing 10% fetal bovine serum, repeatedly blow and filter, centrifuge at 1000xg for 5 minutes, discard the supernatant, repeat once, the cells The pellet was resuspended in epidermis medium and cultured by adding Rock inhibitor Y27632.

[0066] 2) Treatment of epidermal cells with phenformin:

[0067] Take the cultured epidermal cells (P3) and inoculate them in a six-well plate, use 2ml medium for each well of 300,000 cells, replace the medium 24 hours after inoculation, and then use differ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses application of phenformin as a skin anti-inflammatory preparation. First discovery finds that the phenformin can be used for obviously inhibiting skin epidermic cells from secreting various inflammatory cell factors including 6 / 8(IL-6, IL-8) and tumor necrosis factors (TNF alpha), and similarly inflammatory response of human skin epithelial cells induced by frequently-used 12-oxy-tetradecanoyl phorbol-13-acetate (TPA) and poly-poly(C) polyinosinic-polycytidylic acid (poly I:C) (immunologic stimulant), so that the phenformin can be used for preventing and curing related diseases of skin inflammation. Therefore, novel application is developed for the phenformin, and new prevention and treatment means is provided for related diseases of skin inflammation.

Description

technical field [0001] The invention belongs to the field of medicine and pharmacy, and in particular relates to the use of phenformin in preparing anti-inflammatory preparations for skin. Background technique [0002] Dermatitis refers to a general term for skin inflammatory diseases caused by various internal and external infections or non-infectious factors. It is not an independent disease. Its etiology and clinical manifestations are complex and diverse, and it recurs repeatedly, making clinical treatment difficult. According to modern medical research, the etiology of dermatitis diseases is very complicated, which may be related to the following factors: including internal factors such as chronic infection lesions (such as chronic cholecystitis, tonsillitis, intestinal parasitic diseases, etc.), endocrine and metabolic changes (such as menstrual disorders, pregnancy, etc.), blood circulation disorders (such as calf varicose veins, etc.), neuropsychiatric factors, genet...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/155A61P29/00A61P17/00A61P17/04A61P17/10A61P17/02
CPCA61K31/155
Inventor 吴训伟李丁洋周倩郭泾
Owner SHANDONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com