Treatment of mosaic viruses and bacterial infections of plants
A technology of bacteria and plants, applied in botany equipment and methods, plant growth regulators, chemicals for biological control, etc., can solve problems such as the impact of food crop supply
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[0120] Preparation of Microbial Based Products
[0121] A microorganism-based product of the present invention is simply a fermentation broth comprising the microorganisms and / or microbial metabolites and / or any residual nutrients produced by the microorganisms. Fermentation products can be used directly without extraction or purification. Extraction and purification can be readily achieved, if desired, using standard extraction and / or purification methods or techniques described in the literature.
[0122] The microorganisms in the microorganism-based product can be in active or inactive form. Microbial-based products may contain a combination of viable and non-viable microorganisms.
[0123] Microorganisms, microbial growth by-products, and / or broth produced from microbial growth can be removed from the growth vessel and transferred, for example, by piping for immediate use.
[0124] In other embodiments, the composition (microorganisms, metabolites, and / or culture broth)...
Embodiment 1
[0171] Embodiment 1: Sophorolipid fermentation
[0172] For the present invention, a natural mixture of sophorolipids was synthesized by fermenting Candida globosa in a fermentation medium containing 100 grams of glucose, 5 grams of yeast extract, 1 gram of urea and 100 grams of canola oil in 1000 ml of water. After 5-7 days of fermentation, the sophorolipids were collected by precipitation. After adding an additional amount of the same nutrient medium, the fermentation was continued for 3 days, after which another portion of SLP was collected.
[0173] Two different products were produced from this fermentation: one containing pure SLP and one containing a culture of Candida sphaeroides containing SLP.
Embodiment 2
[0174] Example 2: Preparation of SLP solution for foliar treatment
[0175] Precipitated SLP obtained by fermentation of Candida globosa can be used to produce products for the treatment of various types of versicolor virus in plants. Precipitated SLP typically contains no more than 50% water; however, such therapeutic products can be prepared without further concentration. The therapeutic product contains 0.1 to 0.5% (v / v) unpurified SLP.
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